Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Abstract Background Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities. Recent developments in synthetic biology have extended the toolbox for genetic engineering of P. past...

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Bibliographic Details
Main Authors: Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos Luna-Flores, Xiaojing Chen, Juhani von Hellens, Robert Speight
Format: Article
Language:English
Published: BMC 2021-01-01
Series:Microbial Cell Factories
Subjects:
Phytase
Strain engineering
Disulfide bond
Folding
Secretion
Online Access:https://doi.org/10.1186/s12934-020-01499-7

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