The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE
Reactive astrocytes play a complex role in multiple sclerosis, and the astrocytes reactivity is an important factor in the pathogenesis of pain. It is of great significance to explore the genesis and development mechanism of pain in the early stage of multiple sclerosis (MS) for early intervention o...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996122003060 |
| _version_ | 1811177787257520128 |
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| author | Yumeng Ding Liye Hu Xiaotong Wang Qingyu Sun Tingting Hu Jing Liu Danmin Shen Yurui Zhang Wen Chen Chao Wei Meng Liu Dianxin Liu Peipei Wang Chenguang Zhang Jiannan Zhang Qian Li Fei Yang |
| author_facet | Yumeng Ding Liye Hu Xiaotong Wang Qingyu Sun Tingting Hu Jing Liu Danmin Shen Yurui Zhang Wen Chen Chao Wei Meng Liu Dianxin Liu Peipei Wang Chenguang Zhang Jiannan Zhang Qian Li Fei Yang |
| author_sort | Yumeng Ding |
| collection | DOAJ |
| description | Reactive astrocytes play a complex role in multiple sclerosis, and the astrocytes reactivity is an important factor in the pathogenesis of pain. It is of great significance to explore the genesis and development mechanism of pain in the early stage of multiple sclerosis (MS) for early intervention of the disease. This study aims to explore astrocyte reactivity at different stages of the experimental autoimmune encephalomyelitis (EAE) model, a mouse model of MS, and the role of astrocytes in the pain in the early stage of the EAE. In this study, we demonstrated that spinal dorsal horn astrocytes were activated in the pre-clinical stage of EAE mice, and the inhibition of spinal cord astrocyte reactivity effectively alleviates pain symptoms in EAE mice. On the other hand, spinal cord microglia were not directly participated in the early EAE pain. Moreover, the ion channel LRRC8A mediated the reactivity of spinal dorsal horn astrocytes by regulating the STAT3 pathway, therefore playing a role in the early pain of EAE. |
| first_indexed | 2024-04-11T06:07:05Z |
| format | Article |
| id | doaj.art-05df10f77dbf4cb6a89557078504d956 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-04-11T06:07:05Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-05df10f77dbf4cb6a89557078504d9562022-12-22T04:41:27ZengElsevierNeurobiology of Disease1095-953X2022-12-01175105914The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAEYumeng Ding0Liye Hu1Xiaotong Wang2Qingyu Sun3Tingting Hu4Jing Liu5Danmin Shen6Yurui Zhang7Wen Chen8Chao Wei9Meng Liu10Dianxin Liu11Peipei Wang12Chenguang Zhang13Jiannan Zhang14Qian Li15Fei Yang16Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China; Corresponding authors at: School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China; Corresponding authors at: School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.Reactive astrocytes play a complex role in multiple sclerosis, and the astrocytes reactivity is an important factor in the pathogenesis of pain. It is of great significance to explore the genesis and development mechanism of pain in the early stage of multiple sclerosis (MS) for early intervention of the disease. This study aims to explore astrocyte reactivity at different stages of the experimental autoimmune encephalomyelitis (EAE) model, a mouse model of MS, and the role of astrocytes in the pain in the early stage of the EAE. In this study, we demonstrated that spinal dorsal horn astrocytes were activated in the pre-clinical stage of EAE mice, and the inhibition of spinal cord astrocyte reactivity effectively alleviates pain symptoms in EAE mice. On the other hand, spinal cord microglia were not directly participated in the early EAE pain. Moreover, the ion channel LRRC8A mediated the reactivity of spinal dorsal horn astrocytes by regulating the STAT3 pathway, therefore playing a role in the early pain of EAE.http://www.sciencedirect.com/science/article/pii/S0969996122003060Multiple sclerosisPainExperimental autoimmune encephalomyelitisAstrocyteLRRC8A |
| spellingShingle | Yumeng Ding Liye Hu Xiaotong Wang Qingyu Sun Tingting Hu Jing Liu Danmin Shen Yurui Zhang Wen Chen Chao Wei Meng Liu Dianxin Liu Peipei Wang Chenguang Zhang Jiannan Zhang Qian Li Fei Yang The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE Neurobiology of Disease Multiple sclerosis Pain Experimental autoimmune encephalomyelitis Astrocyte LRRC8A |
| title | The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE |
| title_full | The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE |
| title_fullStr | The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE |
| title_full_unstemmed | The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE |
| title_short | The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE |
| title_sort | contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of eae |
| topic | Multiple sclerosis Pain Experimental autoimmune encephalomyelitis Astrocyte LRRC8A |
| url | http://www.sciencedirect.com/science/article/pii/S0969996122003060 |
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