Development of Immunoassay Based on Monoclonal Antibody Reacted with the Neonicotinoid Insecticides Clothianidin and Dinotefuran

Development of Immunoassay Based on Monoclonal Antibody Reacted with the Neonicotinoid Insecticides Clothianidin and Dinotefuran

Enzyme-linked immunosorbent assay (ELISA) based on a monoclonal antibody (MoAb) was developed for the neonicotinoid insecticide clothianidin. A new clothianidin hapten (3-[5-(3-methyl-2-nitroguanidinomethyl)-1,3-thiazol-2-ylthio] propionic acid) was synthesized and conjugated to keyhole limpet hemoc...

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Bibliographic Details
Main Authors:	Seiji Iwasa, Mikiko Uchigashima, Shigekazu Ito, Eiki Watanabe, Shiro Miyake
Format:	Article
Language:	English
Published:	MDPI AG 2012-11-01
Series:	Sensors
Subjects:	enzyme-linked immunosorbent assay ELISA hapten
Online Access:	http://www.mdpi.com/1424-8220/12/11/15858

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