Applications of Virus-Induced Gene Silencing in Cotton

Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that has become an effective tool to investigate gene function in plants. Cotton is one of the most important economic crops globally. In the past decade, VIGS has been successfully applied in cotton functional genomic...

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Main Authors: Yue Tian, Yao Fang, Kaixin Zhang, Zeyang Zhai, Yujie Yang, Meiyu He, Xu Cao
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Plants
Subjects:
Online Access:https://www.mdpi.com/2223-7747/13/2/272
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author Yue Tian
Yao Fang
Kaixin Zhang
Zeyang Zhai
Yujie Yang
Meiyu He
Xu Cao
author_facet Yue Tian
Yao Fang
Kaixin Zhang
Zeyang Zhai
Yujie Yang
Meiyu He
Xu Cao
author_sort Yue Tian
collection DOAJ
description Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that has become an effective tool to investigate gene function in plants. Cotton is one of the most important economic crops globally. In the past decade, VIGS has been successfully applied in cotton functional genomic studies, including those examining abiotic and biotic stress responses and vegetative and reproductive development. This article summarizes the traditional vectors used in the cotton VIGS system, the visible markers used for endogenous gene silencing, the applications of VIGS in cotton functional genomics, and the limitations of VIGS and how they can be addressed in cotton.
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spelling doaj.art-05dfed510f5b419c864bfc083f8c2db42024-01-29T14:11:42ZengMDPI AGPlants2223-77472024-01-0113227210.3390/plants13020272Applications of Virus-Induced Gene Silencing in CottonYue Tian0Yao Fang1Kaixin Zhang2Zeyang Zhai3Yujie Yang4Meiyu He5Xu Cao6College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaVirus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that has become an effective tool to investigate gene function in plants. Cotton is one of the most important economic crops globally. In the past decade, VIGS has been successfully applied in cotton functional genomic studies, including those examining abiotic and biotic stress responses and vegetative and reproductive development. This article summarizes the traditional vectors used in the cotton VIGS system, the visible markers used for endogenous gene silencing, the applications of VIGS in cotton functional genomics, and the limitations of VIGS and how they can be addressed in cotton.https://www.mdpi.com/2223-7747/13/2/272cottonvirus-induced gene silencingfunctional genomics
spellingShingle Yue Tian
Yao Fang
Kaixin Zhang
Zeyang Zhai
Yujie Yang
Meiyu He
Xu Cao
Applications of Virus-Induced Gene Silencing in Cotton
Plants
cotton
virus-induced gene silencing
functional genomics
title Applications of Virus-Induced Gene Silencing in Cotton
title_full Applications of Virus-Induced Gene Silencing in Cotton
title_fullStr Applications of Virus-Induced Gene Silencing in Cotton
title_full_unstemmed Applications of Virus-Induced Gene Silencing in Cotton
title_short Applications of Virus-Induced Gene Silencing in Cotton
title_sort applications of virus induced gene silencing in cotton
topic cotton
virus-induced gene silencing
functional genomics
url https://www.mdpi.com/2223-7747/13/2/272
work_keys_str_mv AT yuetian applicationsofvirusinducedgenesilencingincotton
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AT zeyangzhai applicationsofvirusinducedgenesilencingincotton
AT yujieyang applicationsofvirusinducedgenesilencingincotton
AT meiyuhe applicationsofvirusinducedgenesilencingincotton
AT xucao applicationsofvirusinducedgenesilencingincotton