Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in Spain
<i>Chlamydia trachomatis</i> infection is an important public health problem. Our objective was to assess the dynamics of the transmission of this infection, analysing the distribution of circulating <i>ompA</i> genotypes and multilocus sequence types of <i>C. trachomat...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/8/6941 |
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| author | Luis Piñeiro Laura Villa Paula Salmerón Maria Dolores Maciá Luis Otero Martí Vall-Mayans Ana Milagro Samuel Bernal Ayla Manzanal Iñigo Ansa Gustavo Cilla |
| author_facet | Luis Piñeiro Laura Villa Paula Salmerón Maria Dolores Maciá Luis Otero Martí Vall-Mayans Ana Milagro Samuel Bernal Ayla Manzanal Iñigo Ansa Gustavo Cilla |
| author_sort | Luis Piñeiro |
| collection | DOAJ |
| description | <i>Chlamydia trachomatis</i> infection is an important public health problem. Our objective was to assess the dynamics of the transmission of this infection, analysing the distribution of circulating <i>ompA</i> genotypes and multilocus sequence types of <i>C. trachomatis</i> in Spain as a function of clinical and epidemiological variables. During 2018 and 2019, we genetically characterized <i>C. trachomatis</i> in tertiary hospitals in six areas in Spain (Asturias, Barcelona, Gipuzkoa, Mallorca, Seville and Zaragoza), with a catchment population of 3.050 million people. Genotypes and sequence types were obtained using polymerase chain reaction techniques that amplify a fragment of the <i>ompA</i> gene, and five highly variable genes (<i>hctB</i>, CT058, CT144, CT172 and <i>pbpB</i>), respectively. Amplicons were sequenced and phylogenetic analysis was conducted. We obtained genotypes in 636/698 cases (91.1%). Overall and by area, genotype E was the most common (35%). Stratifying by sex, genotypes D and G were more common among men, and genotypes F and I among women (<i>p</i> < 0.05). Genotypes D, G and J were more common in men who have sex with men (MSM) than in men who have sex with women (MSW), in whom the most common genotypes were E and F. The diversity index was higher in sequence typing (0.981) than in genotyping (0.791), and the most common sequence types were ST52 and ST108 in MSM, and ST30, ST148, ST276 and ST327 in MSW. Differences in genotype distribution between geographical areas were attributable to differences in population characteristics. The transmission dynamics varied with sexual behaviour: the predominant genotypes and most frequent sequence types found in MSM were different to those detected in MSW and women. |
| first_indexed | 2024-03-11T04:56:43Z |
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| id | doaj.art-05e2c428f10b43af83bd92ecf12ee4e0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:56:43Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-05e2c428f10b43af83bd92ecf12ee4e02023-11-17T19:32:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248694110.3390/ijms24086941Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in SpainLuis Piñeiro0Laura Villa1Paula Salmerón2Maria Dolores Maciá3Luis Otero4Martí Vall-Mayans5Ana Milagro6Samuel Bernal7Ayla Manzanal8Iñigo Ansa9Gustavo Cilla10Microbiology Department, Donostia University Hospital-Biodonostia Health Research Institute, 20014 San Sebastian, SpainSexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), 28003 Madrid, SpainSexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), 28003 Madrid, SpainSexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), 28003 Madrid, SpainSexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), 28003 Madrid, SpainSexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), 28003 Madrid, SpainSexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), 28003 Madrid, SpainSexually Transmitted Infections Study Group of the Infectious Diseases and Clinical Microbiology Spanish Society (GEITS-SEIMC), 28003 Madrid, SpainMicrobiology Department, Donostia University Hospital-Biodonostia Health Research Institute, 20014 San Sebastian, SpainMicrobiology Department, Donostia University Hospital-Biodonostia Health Research Institute, 20014 San Sebastian, SpainMicrobiology Department, Donostia University Hospital-Biodonostia Health Research Institute, 20014 San Sebastian, Spain<i>Chlamydia trachomatis</i> infection is an important public health problem. Our objective was to assess the dynamics of the transmission of this infection, analysing the distribution of circulating <i>ompA</i> genotypes and multilocus sequence types of <i>C. trachomatis</i> in Spain as a function of clinical and epidemiological variables. During 2018 and 2019, we genetically characterized <i>C. trachomatis</i> in tertiary hospitals in six areas in Spain (Asturias, Barcelona, Gipuzkoa, Mallorca, Seville and Zaragoza), with a catchment population of 3.050 million people. Genotypes and sequence types were obtained using polymerase chain reaction techniques that amplify a fragment of the <i>ompA</i> gene, and five highly variable genes (<i>hctB</i>, CT058, CT144, CT172 and <i>pbpB</i>), respectively. Amplicons were sequenced and phylogenetic analysis was conducted. We obtained genotypes in 636/698 cases (91.1%). Overall and by area, genotype E was the most common (35%). Stratifying by sex, genotypes D and G were more common among men, and genotypes F and I among women (<i>p</i> < 0.05). Genotypes D, G and J were more common in men who have sex with men (MSM) than in men who have sex with women (MSW), in whom the most common genotypes were E and F. The diversity index was higher in sequence typing (0.981) than in genotyping (0.791), and the most common sequence types were ST52 and ST108 in MSM, and ST30, ST148, ST276 and ST327 in MSW. Differences in genotype distribution between geographical areas were attributable to differences in population characteristics. The transmission dynamics varied with sexual behaviour: the predominant genotypes and most frequent sequence types found in MSM were different to those detected in MSW and women.https://www.mdpi.com/1422-0067/24/8/6941<i>Chlamydia trachomatis</i><i>ompA</i> genotypingmultilocus sequence typingsexual behaviour |
| spellingShingle | Luis Piñeiro Laura Villa Paula Salmerón Maria Dolores Maciá Luis Otero Martí Vall-Mayans Ana Milagro Samuel Bernal Ayla Manzanal Iñigo Ansa Gustavo Cilla Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in Spain International Journal of Molecular Sciences <i>Chlamydia trachomatis</i> <i>ompA</i> genotyping multilocus sequence typing sexual behaviour |
| title | Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in Spain |
| title_full | Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in Spain |
| title_fullStr | Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in Spain |
| title_full_unstemmed | Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in Spain |
| title_short | Genetic Characterization of Non-<i>Lymphogranuloma venereum Chlamydia trachomatis</i> Indicates Distinct Infection Transmission Networks in Spain |
| title_sort | genetic characterization of non i lymphogranuloma venereum chlamydia trachomatis i indicates distinct infection transmission networks in spain |
| topic | <i>Chlamydia trachomatis</i> <i>ompA</i> genotyping multilocus sequence typing sexual behaviour |
| url | https://www.mdpi.com/1422-0067/24/8/6941 |
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