| Summary: | In spite of ongoing eradication programs, helminth infections are still a medical issue in Ghana. For follow-up assessments on the decline of regional helminth infections, historic baseline prevalence values obtained with standardized diagnostic procedures can be helpful. In this retrospective cross-sectional study, real-time PCR targeting the nematodes <i>Ancylostoma</i> spp. (ITS2), <i>Ascaris lumbricoides</i> (ITS1), <i>Enterobius vermicularis</i> (ITS1), <i>Necator americanus</i> (ITS2), <i>Strongyloides stercoralis</i> (18S rRNA) and <i>Trichuris trichiura</i> (18S rRNA), the trematodes <i>Schistosoma</i> spp. (ITS2) as well as the cestodes <i>Hymenolepis nana</i> (ITS1), <i>Taenia saginata</i> (ITS1) and <i>Taenia solium</i> (ITS1) was applied with 2046 DNA eluates from stool samples of Ghanaian children from the Ashanti region collected between 2007 and 2008 in order to retrospectively define prevalence values. The overall prevalence was low with 3.8% (<i>n</i> = 77) and only 0.1% (<i>n</i> = 2) double infections with helminths were recorded. The three most frequently detected enteric helminth species comprised 2% <i>S. stercoralis</i> (<i>n</i> = 41), 0.8% <i>H. nana</i> (<i>n</i> = 16), and 0.7% <i>N. americanus</i> (<i>n</i> = 14), while only sporadic infection events were recorded for other helminth species comprising 0.1% <i>E. vermicularis</i> (<i>n</i> = 2), 0.1% <i>Schistosoma</i> spp. (<i>n</i> = 2), 0.1% <i>T. saginata</i> (<i>n</i> = 1) and 0.1% <i>T. trichiura</i> (<i>n</i> = 1). <i>A. lumbricoides</i>, <i>Ancylostoma</i> spp. and <i>T. solium</i> were not detected at all. In conclusion, the retrospective assessment suggests a low prevalence of enteric helminth infections in Ghanaian children from the Ashanti Region within the assessment period between 2007 and 2008.
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