Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage
Tip-burn has seriously affected the yield, quality and commodity value of mini Chinese cabbage. Calcium (Ca2+) deficiency is the main cause of tip-burn. In order to investigate whether exogenous brassinosteroids (BRs) can alleviate tip-burn induced by calcium (Ca2+) deficiency and its mechanism, in...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.999051/full |
| _version_ | 1811205268111884288 |
|---|---|
| author | Yutong Li Yutong Li Jizhong Ma Xueqin Gao Jianzhong Tie Yue Wu Zhongqi Tang Linli Hu Linli Hu Jihua Yu Jihua Yu |
| author_facet | Yutong Li Yutong Li Jizhong Ma Xueqin Gao Jianzhong Tie Yue Wu Zhongqi Tang Linli Hu Linli Hu Jihua Yu Jihua Yu |
| author_sort | Yutong Li |
| collection | DOAJ |
| description | Tip-burn has seriously affected the yield, quality and commodity value of mini Chinese cabbage. Calcium (Ca2+) deficiency is the main cause of tip-burn. In order to investigate whether exogenous brassinosteroids (BRs) can alleviate tip-burn induced by calcium (Ca2+) deficiency and its mechanism, in this study, Ca2+ deficiency in nutrient solution was used to induced tip-burn, and then distilled water and BRs were sprayed on leaves to observe the tip-burn incidence of mini Chinese cabbage. The tip-burn incidence and disease index, leaf area, fluorescence parameters (Fv/Fm, NPQ, qP andφPSII) and gas exchange parameters (Tr, Pn, Gs and Ci), pigment contents, cell wall components, mesophyll cell ultrastructure and the expression of genes related to chlorophyll degradation were measured. The results showed that exogenous BRs reduced the tip-burn incidence rate and disease index of mini Chinese cabbage, and the tip-burn incidence rate reached the highest on the ninth day after treatment. Exogenous BRs increased the contents of cellulose, hemifiber, water-soluble pectin in Ca2+ deficiency treated leaves, maintaining the stability of cell wall structure. In addition, BRs increased photosynthetic rate by increasing the activities of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and fructose 1,6-bisphosphatase (FBPase) related to Calvin cycle, maintaining relatively complete chloroplast structure and higher chlorophyll content via down-regulating the expression of BrPPH1 and BrPAO1 genes related to chlorophyll degradation. In conclusion, exogenous BRs alleviated calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis. |
| first_indexed | 2024-04-12T03:27:55Z |
| format | Article |
| id | doaj.art-05e599c640e64df09279ac2613916211 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T03:27:55Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-05e599c640e64df09279ac26139162112022-12-22T03:49:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.999051999051Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese CabbageYutong Li0Yutong Li1Jizhong Ma2Xueqin Gao3Jianzhong Tie4Yue Wu5Zhongqi Tang6Linli Hu7Linli Hu8Jihua Yu9Jihua Yu10Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaTip-burn has seriously affected the yield, quality and commodity value of mini Chinese cabbage. Calcium (Ca2+) deficiency is the main cause of tip-burn. In order to investigate whether exogenous brassinosteroids (BRs) can alleviate tip-burn induced by calcium (Ca2+) deficiency and its mechanism, in this study, Ca2+ deficiency in nutrient solution was used to induced tip-burn, and then distilled water and BRs were sprayed on leaves to observe the tip-burn incidence of mini Chinese cabbage. The tip-burn incidence and disease index, leaf area, fluorescence parameters (Fv/Fm, NPQ, qP andφPSII) and gas exchange parameters (Tr, Pn, Gs and Ci), pigment contents, cell wall components, mesophyll cell ultrastructure and the expression of genes related to chlorophyll degradation were measured. The results showed that exogenous BRs reduced the tip-burn incidence rate and disease index of mini Chinese cabbage, and the tip-burn incidence rate reached the highest on the ninth day after treatment. Exogenous BRs increased the contents of cellulose, hemifiber, water-soluble pectin in Ca2+ deficiency treated leaves, maintaining the stability of cell wall structure. In addition, BRs increased photosynthetic rate by increasing the activities of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and fructose 1,6-bisphosphatase (FBPase) related to Calvin cycle, maintaining relatively complete chloroplast structure and higher chlorophyll content via down-regulating the expression of BrPPH1 and BrPAO1 genes related to chlorophyll degradation. In conclusion, exogenous BRs alleviated calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis.https://www.frontiersin.org/articles/10.3389/fpls.2022.999051/fullTip-burndeficiency calciumbrassinosteroidscell wall structuralphotosynthesis |
| spellingShingle | Yutong Li Yutong Li Jizhong Ma Xueqin Gao Jianzhong Tie Yue Wu Zhongqi Tang Linli Hu Linli Hu Jihua Yu Jihua Yu Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage Frontiers in Plant Science Tip-burn deficiency calcium brassinosteroids cell wall structural photosynthesis |
| title | Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage |
| title_full | Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage |
| title_fullStr | Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage |
| title_full_unstemmed | Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage |
| title_short | Exogenous brassinosteroids alleviate calcium deficiency-induced tip-burn by maintaining cell wall structural stability and higher photosynthesis in mini Chinese Cabbage |
| title_sort | exogenous brassinosteroids alleviate calcium deficiency induced tip burn by maintaining cell wall structural stability and higher photosynthesis in mini chinese cabbage |
| topic | Tip-burn deficiency calcium brassinosteroids cell wall structural photosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.999051/full |
| work_keys_str_mv | AT yutongli exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT yutongli exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT jizhongma exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT xueqingao exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT jianzhongtie exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT yuewu exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT zhongqitang exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT linlihu exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT linlihu exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT jihuayu exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage AT jihuayu exogenousbrassinosteroidsalleviatecalciumdeficiencyinducedtipburnbymaintainingcellwallstructuralstabilityandhigherphotosynthesisinminichinesecabbage |