Electron microscopic study of the role of lipid micelles in intestinal fat absorption
In vitro micellar solutions of oleic acid, monoolein, and sodium taurocholate were studied electron microscopically. They contained osmiophilic particles 30-200A in diameter. Osmium staining alone was sufficient to demonstrate the particles; lead staining had little effect on their appearance.The in...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1966-07-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520392555 |
| _version_ | 1818941162513235968 |
|---|---|
| author | C.T. Ashworth J.F. Lawrence |
| author_facet | C.T. Ashworth J.F. Lawrence |
| author_sort | C.T. Ashworth |
| collection | DOAJ |
| description | In vitro micellar solutions of oleic acid, monoolein, and sodium taurocholate were studied electron microscopically. They contained osmiophilic particles 30-200A in diameter. Osmium staining alone was sufficient to demonstrate the particles; lead staining had little effect on their appearance.The intestinal intraluminal contents from rats during the absorption of unsaturated fat also contained osmiophilic particles, 40-200A, and numerous similar particles were found between the microvilli and engaged in the fine filamentous coating of microvilli. In the lumen only, larger emulsion-type droplets were also seen. The small particles were demonstrable in osmium-fixed material, with or without lead stains, and staining with lead only increased contrast of the particles.Spherules measuring about 1000A in diameter with walls about 100A thick were observed in the terminal web during fat absorption, at which time they were slightly larger and more numerous than in fasting rats.It is proposed that during fat absorption micellar particles are engaged in the filamentous material covering the microvilli and then enter the absorptive cell either by molecular diffusion across the plasma membrane or by being incorporated into the walls of thick-coated spherules which then pass into the subapical cytoplasm. |
| first_indexed | 2024-12-20T06:51:09Z |
| format | Article |
| id | doaj.art-05e65f50a4014f7ca6eb3e262bd2f4af |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T06:51:09Z |
| publishDate | 1966-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-05e65f50a4014f7ca6eb3e262bd2f4af2022-12-21T19:49:31ZengElsevierJournal of Lipid Research0022-22751966-07-0174465472Electron microscopic study of the role of lipid micelles in intestinal fat absorptionC.T. Ashworth0J.F. Lawrence1Department of Pathology, The University of Texas Southwestern Medical School, Dallas, TexasDepartment of Pathology, The University of Texas Southwestern Medical School, Dallas, TexasIn vitro micellar solutions of oleic acid, monoolein, and sodium taurocholate were studied electron microscopically. They contained osmiophilic particles 30-200A in diameter. Osmium staining alone was sufficient to demonstrate the particles; lead staining had little effect on their appearance.The intestinal intraluminal contents from rats during the absorption of unsaturated fat also contained osmiophilic particles, 40-200A, and numerous similar particles were found between the microvilli and engaged in the fine filamentous coating of microvilli. In the lumen only, larger emulsion-type droplets were also seen. The small particles were demonstrable in osmium-fixed material, with or without lead stains, and staining with lead only increased contrast of the particles.Spherules measuring about 1000A in diameter with walls about 100A thick were observed in the terminal web during fat absorption, at which time they were slightly larger and more numerous than in fasting rats.It is proposed that during fat absorption micellar particles are engaged in the filamentous material covering the microvilli and then enter the absorptive cell either by molecular diffusion across the plasma membrane or by being incorporated into the walls of thick-coated spherules which then pass into the subapical cytoplasm.http://www.sciencedirect.com/science/article/pii/S0022227520392555lipid absorptionelectron microscopymicellesin vitroin vivorat |
| spellingShingle | C.T. Ashworth J.F. Lawrence Electron microscopic study of the role of lipid micelles in intestinal fat absorption Journal of Lipid Research lipid absorption electron microscopy micelles in vitro in vivo rat |
| title | Electron microscopic study of the role of lipid micelles in intestinal fat absorption |
| title_full | Electron microscopic study of the role of lipid micelles in intestinal fat absorption |
| title_fullStr | Electron microscopic study of the role of lipid micelles in intestinal fat absorption |
| title_full_unstemmed | Electron microscopic study of the role of lipid micelles in intestinal fat absorption |
| title_short | Electron microscopic study of the role of lipid micelles in intestinal fat absorption |
| title_sort | electron microscopic study of the role of lipid micelles in intestinal fat absorption |
| topic | lipid absorption electron microscopy micelles in vitro in vivo rat |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520392555 |
| work_keys_str_mv | AT ctashworth electronmicroscopicstudyoftheroleoflipidmicellesinintestinalfatabsorption AT jflawrence electronmicroscopicstudyoftheroleoflipidmicellesinintestinalfatabsorption |