Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats
Abstract Background Researches have used intra-compartmental infusion and ballon tourniquest to create high intra-compartmental pressure in animal models of Acute Compartment Syndrome (ACS). However, due to the large differences in the modeling methods and the evaluation criteria of ACS, further res...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12891-024-07187-6 |
| _version_ | 1797350175526617088 |
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| author | Qi Dong Yubin Long Lin Jin Guanlin Hou Guoqiang Li Tao Wang Huiyang Jia Yingchao Yin Junfei Guo Huijie Ma Sujuan Xu Yingze Zhang Zhiyong Hou |
| author_facet | Qi Dong Yubin Long Lin Jin Guanlin Hou Guoqiang Li Tao Wang Huiyang Jia Yingchao Yin Junfei Guo Huijie Ma Sujuan Xu Yingze Zhang Zhiyong Hou |
| author_sort | Qi Dong |
| collection | DOAJ |
| description | Abstract Background Researches have used intra-compartmental infusion and ballon tourniquest to create high intra-compartmental pressure in animal models of Acute Compartment Syndrome (ACS). However, due to the large differences in the modeling methods and the evaluation criteria of ACS, further researches of its pathophysiology and pathogenesis are hindered. Currently, there is no ideal animal model for ACS and this study aimed to establish a reproducible, clinically relevant animal model. Methods Blunt trauma and fracture were caused by the free falling of weights (0.5 kg, 1 kg, 2 kg) from a height of 40 cm onto the lower legs of rats, and the application of pressures of 100 mmHg, 200 mmHg, 300 mmHg and 400 mmHg to the lower limbs of rats using a modified pressurizing device for 6 h. The intra-compartmental pressure (ICP) and the pressure change (ΔP) of rats with single and combined injury were continuously recorded, and the pathophysiology of the rats was assessed based on serum biochemistry, histological and hemodynamic changes. Results The ΔP caused by single injury method of different weights falling onto the lower leg did not meet the diagnosis criteria for ACS (< 30 mmHg). On the other hand, a combined injury method of a falling weight of 1.0 kg and the use of a pressurizing device with pressure of 300 mmHg or 400 mmHg for 6 h resulted in the desired ACS diagnosis criteria with a ΔP value of less than 30 mmHg. The serum analytes, histological damage score, and fibrosis level of the combined injury group were significantly increased compared with control group, while the blood flow was significantly decreased compared with control group. Conclusion We successfully established a new preclinical ACS-like rat model, by the compression of the lower leg of rats with 300 mmHg pressure for 6 h and blunt trauma by 1.0 kg weight falling. |
| first_indexed | 2024-03-08T12:40:58Z |
| format | Article |
| id | doaj.art-05e843e325cd439297d52a54ba291bf8 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-03-08T12:40:58Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-05e843e325cd439297d52a54ba291bf82024-01-21T12:07:14ZengBMCBMC Musculoskeletal Disorders1471-24742024-01-0125111210.1186/s12891-024-07187-6Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in ratsQi Dong0Yubin Long1Lin Jin2Guanlin Hou3Guoqiang Li4Tao Wang5Huiyang Jia6Yingchao Yin7Junfei Guo8Huijie Ma9Sujuan Xu10Yingze Zhang11Zhiyong Hou12Department of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityWLSA Shanghai AcademyDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Joint Surgery, Honghui Hospital, Xi’an Jiaotong UniversityHebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityDepartment of Orthopaedics Surgery, Third Hospital of Hebei Medical UniversityAbstract Background Researches have used intra-compartmental infusion and ballon tourniquest to create high intra-compartmental pressure in animal models of Acute Compartment Syndrome (ACS). However, due to the large differences in the modeling methods and the evaluation criteria of ACS, further researches of its pathophysiology and pathogenesis are hindered. Currently, there is no ideal animal model for ACS and this study aimed to establish a reproducible, clinically relevant animal model. Methods Blunt trauma and fracture were caused by the free falling of weights (0.5 kg, 1 kg, 2 kg) from a height of 40 cm onto the lower legs of rats, and the application of pressures of 100 mmHg, 200 mmHg, 300 mmHg and 400 mmHg to the lower limbs of rats using a modified pressurizing device for 6 h. The intra-compartmental pressure (ICP) and the pressure change (ΔP) of rats with single and combined injury were continuously recorded, and the pathophysiology of the rats was assessed based on serum biochemistry, histological and hemodynamic changes. Results The ΔP caused by single injury method of different weights falling onto the lower leg did not meet the diagnosis criteria for ACS (< 30 mmHg). On the other hand, a combined injury method of a falling weight of 1.0 kg and the use of a pressurizing device with pressure of 300 mmHg or 400 mmHg for 6 h resulted in the desired ACS diagnosis criteria with a ΔP value of less than 30 mmHg. The serum analytes, histological damage score, and fibrosis level of the combined injury group were significantly increased compared with control group, while the blood flow was significantly decreased compared with control group. Conclusion We successfully established a new preclinical ACS-like rat model, by the compression of the lower leg of rats with 300 mmHg pressure for 6 h and blunt trauma by 1.0 kg weight falling.https://doi.org/10.1186/s12891-024-07187-6Acute compartment sundromeAnimal modelsICP |
| spellingShingle | Qi Dong Yubin Long Lin Jin Guanlin Hou Guoqiang Li Tao Wang Huiyang Jia Yingchao Yin Junfei Guo Huijie Ma Sujuan Xu Yingze Zhang Zhiyong Hou Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats BMC Musculoskeletal Disorders Acute compartment sundrome Animal models ICP |
| title | Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats |
| title_full | Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats |
| title_fullStr | Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats |
| title_full_unstemmed | Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats |
| title_short | Establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats |
| title_sort | establishment and pathophysiological evaluation of a novel model of acute compartment syndrome in rats |
| topic | Acute compartment sundrome Animal models ICP |
| url | https://doi.org/10.1186/s12891-024-07187-6 |
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