Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves
The present study was aimed at the detection and describing the lesions of akabane virus in foetal and new-born calves tissues using immunohistochemical and immunofluorescence techniques. Akabane virus lesions were evaluated in 12 foetuses and three new-born calves using serological and pathological...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2014-05-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201405-0002_immunohistochemical-evaluation-of-akabane-virus-infection-in-aborted-and-new-born-calves.php |
| _version_ | 1797897560566792192 |
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| author | M. Haligur S. Hasircioglu O. Ozmen M. Kale A. Aydogan |
| author_facet | M. Haligur S. Hasircioglu O. Ozmen M. Kale A. Aydogan |
| author_sort | M. Haligur |
| collection | DOAJ |
| description | The present study was aimed at the detection and describing the lesions of akabane virus in foetal and new-born calves tissues using immunohistochemical and immunofluorescence techniques. Akabane virus lesions were evaluated in 12 foetuses and three new-born calves using serological and pathological methods and immunohistochemistry and immunofluorescence. Macroscopically, prominent arthrogryposis and hydranencephaly (A-H syndrome) were the main symptoms. At the histopathological examination, lesions were especially localised in the midbrain, pons, and medulla oblongata in the central nervous system (CNS) in calves naturally infected with akabane virus. In these areas, degenerative and necrotic neurons were observed. There was prominent mononuclear infiltration in perivascular areas. While akabane virus antigen was only detected in brain using immunofluorescence, immunohistochemistry against akabane virus yielded positive antigenic reactions in the cerebrum, cerebellum, liver, spleen and kidneys. In addition to these findings, there was a relationship between akabane virus infection and neurofilament (NF), glial fibrillary acidic protein (GFAP), and N-methyl-D-aspartate receptor (NMDAR) immunoreaction in astrocytes and neurons. |
| first_indexed | 2024-04-10T07:59:15Z |
| format | Article |
| id | doaj.art-05ea10d94e8e4da88746be81c7394268 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:15Z |
| publishDate | 2014-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-05ea10d94e8e4da88746be81c73942682023-02-23T03:50:16ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922014-05-0159523023810.17221/7516-VETMEDvet-201405-0002Immunohistochemical evaluation of akabane virus infection in aborted and new-born calvesM. Haligur0S. Hasircioglu1O. Ozmen2M. Kale3A. Aydogan4Faculty of Ceyhan Veterinary Medicine, University of Cukurova, Saricam, Adana, TurkeyFaculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Istiklal Yerleskesi, Burdur, TurkeyFaculty of Ceyhan Veterinary Medicine, University of Cukurova, Saricam, Adana, TurkeyFaculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Istiklal Yerleskesi, Burdur, TurkeyFaculty of Ceyhan Veterinary Medicine, University of Cukurova, Saricam, Adana, TurkeyThe present study was aimed at the detection and describing the lesions of akabane virus in foetal and new-born calves tissues using immunohistochemical and immunofluorescence techniques. Akabane virus lesions were evaluated in 12 foetuses and three new-born calves using serological and pathological methods and immunohistochemistry and immunofluorescence. Macroscopically, prominent arthrogryposis and hydranencephaly (A-H syndrome) were the main symptoms. At the histopathological examination, lesions were especially localised in the midbrain, pons, and medulla oblongata in the central nervous system (CNS) in calves naturally infected with akabane virus. In these areas, degenerative and necrotic neurons were observed. There was prominent mononuclear infiltration in perivascular areas. While akabane virus antigen was only detected in brain using immunofluorescence, immunohistochemistry against akabane virus yielded positive antigenic reactions in the cerebrum, cerebellum, liver, spleen and kidneys. In addition to these findings, there was a relationship between akabane virus infection and neurofilament (NF), glial fibrillary acidic protein (GFAP), and N-methyl-D-aspartate receptor (NMDAR) immunoreaction in astrocytes and neurons.https://vetmed.agriculturejournals.cz/artkey/vet-201405-0002_immunohistochemical-evaluation-of-akabane-virus-infection-in-aborted-and-new-born-calves.phpakabane virusfoetuscalvesimmunofluorescenceimmunoperoxidasepathologyvirology |
| spellingShingle | M. Haligur S. Hasircioglu O. Ozmen M. Kale A. Aydogan Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves Veterinární Medicína akabane virus foetus calves immunofluorescence immunoperoxidase pathology virology |
| title | Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves |
| title_full | Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves |
| title_fullStr | Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves |
| title_full_unstemmed | Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves |
| title_short | Immunohistochemical evaluation of akabane virus infection in aborted and new-born calves |
| title_sort | immunohistochemical evaluation of akabane virus infection in aborted and new born calves |
| topic | akabane virus foetus calves immunofluorescence immunoperoxidase pathology virology |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201405-0002_immunohistochemical-evaluation-of-akabane-virus-infection-in-aborted-and-new-born-calves.php |
| work_keys_str_mv | AT mhaligur immunohistochemicalevaluationofakabanevirusinfectioninabortedandnewborncalves AT shasircioglu immunohistochemicalevaluationofakabanevirusinfectioninabortedandnewborncalves AT oozmen immunohistochemicalevaluationofakabanevirusinfectioninabortedandnewborncalves AT mkale immunohistochemicalevaluationofakabanevirusinfectioninabortedandnewborncalves AT aaydogan immunohistochemicalevaluationofakabanevirusinfectioninabortedandnewborncalves |