XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima.
We show that the geophilomorph centipede Strigamia maritima possesses an XX/XY system of sex chromosomes, with males being the heterogametic sex. This is, to our knowledge, the first report of sex chromosomes in any geophilomorph centipede. Using the recently assembled Strigamia genome sequence, we...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4769173?pdf=render |
| _version_ | 1818144826793656320 |
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| author | Jack E Green Martina Dalíková Ken Sahara František Marec Michael Akam |
| author_facet | Jack E Green Martina Dalíková Ken Sahara František Marec Michael Akam |
| author_sort | Jack E Green |
| collection | DOAJ |
| description | We show that the geophilomorph centipede Strigamia maritima possesses an XX/XY system of sex chromosomes, with males being the heterogametic sex. This is, to our knowledge, the first report of sex chromosomes in any geophilomorph centipede. Using the recently assembled Strigamia genome sequence, we identified a set of scaffolds differentially represented in male and female DNA sequence. Using quantitative real-time PCR, we confirmed that three candidate X chromosome-derived scaffolds are present at approximately twice the copy number in females as in males. Furthermore, we confirmed that six candidate Y chromosome-derived scaffolds contain male-specific sequences. Finally, using this molecular information, we designed an X chromosome-specific DNA probe and performed fluorescent in situ hybridization against mitotic and meiotic chromosome spreads to identify the Strigamia XY sex-chromosome pair cytologically. We found that the X and Y chromosomes are recognizably different in size during the early pachytene stage of meiosis, and exhibit incomplete and delayed pairing. |
| first_indexed | 2024-12-11T11:53:44Z |
| format | Article |
| id | doaj.art-05eb939bf2014735b2469280499fd3b3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T11:53:44Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-05eb939bf2014735b2469280499fd3b32022-12-22T01:08:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01112e015029210.1371/journal.pone.0150292XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima.Jack E GreenMartina DalíkováKen SaharaFrantišek MarecMichael AkamWe show that the geophilomorph centipede Strigamia maritima possesses an XX/XY system of sex chromosomes, with males being the heterogametic sex. This is, to our knowledge, the first report of sex chromosomes in any geophilomorph centipede. Using the recently assembled Strigamia genome sequence, we identified a set of scaffolds differentially represented in male and female DNA sequence. Using quantitative real-time PCR, we confirmed that three candidate X chromosome-derived scaffolds are present at approximately twice the copy number in females as in males. Furthermore, we confirmed that six candidate Y chromosome-derived scaffolds contain male-specific sequences. Finally, using this molecular information, we designed an X chromosome-specific DNA probe and performed fluorescent in situ hybridization against mitotic and meiotic chromosome spreads to identify the Strigamia XY sex-chromosome pair cytologically. We found that the X and Y chromosomes are recognizably different in size during the early pachytene stage of meiosis, and exhibit incomplete and delayed pairing.http://europepmc.org/articles/PMC4769173?pdf=render |
| spellingShingle | Jack E Green Martina Dalíková Ken Sahara František Marec Michael Akam XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima. PLoS ONE |
| title | XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima. |
| title_full | XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima. |
| title_fullStr | XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima. |
| title_full_unstemmed | XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima. |
| title_short | XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima. |
| title_sort | xx xy system of sex determination in the geophilomorph centipede strigamia maritima |
| url | http://europepmc.org/articles/PMC4769173?pdf=render |
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