Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder
Background: Post-traumatic stress disorder (PTSD) is a stress-related psychosomatic disorder caused by occurrence of a traumatic event and the hippocampus volume of the patients with Post-traumatic stress disorder decreased. However, the mechanisms that cause such damage are not well-understood. The...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=42;epage=42;aulast=Alani |
| _version_ | 1811194665034055680 |
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| author | Behrang Alani Nader Maghsoudi Ali Khatibi Mahdi Noureddini Farzad Asefifar Jamal Shams |
| author_facet | Behrang Alani Nader Maghsoudi Ali Khatibi Mahdi Noureddini Farzad Asefifar Jamal Shams |
| author_sort | Behrang Alani |
| collection | DOAJ |
| description | Background: Post-traumatic stress disorder (PTSD) is a stress-related psychosomatic disorder caused by occurrence of a traumatic event and the hippocampus volume of the patients with Post-traumatic stress disorder decreased. However, the mechanisms that cause such damage are not well-understood. The aim of this study is to detect the expression of apoptosis-related Bax, Bcl-2, Caspase-3 and Insulin-like growth Factor-I proteins in the hippocampus region in the Predatory stress rats.
Materials and Methods: A total of 70 male wistar rats were divided into Predatory stress groups of 1d, 2d, 3d, 7d, 14d, 30d and a normal control group (N = 10). Rats were subjected to 5 min of predatory stress and then exposed to the elevated plus-maze (EPM). Serum corticosterone and Insulin-like growth factor-1 level of Hippocampus were measured by ELISA technique. The expression of Bax, Bcl-2, and Caspase-3 were detected by western blotting.
Results: Rats spent significantly more time in closed arms of the elevated plus maze (EPM) than control group after exposure to stress. Serum levels of corticosterone significantly increased at 2d-3d. The expression of hippocampal IGF-1 was significantly up-regulated at 1d-2d after stress. Both Bax and the ratio of Bax/Bcl-2 significantly peaked at Predatory stress 2d-14d. Caspase3 was significantly active among 2d-30 compared to the normal control.
Conclusion: The activation of caspase-3 in the stress groups indicates that apoptosis may be one of the reasons inducing hippocampus atrophy and play roles in the pathogenesis of PTSD. Increase in hippocampus levels of IGF-1 during early PTSD might be involved in the early molecular inhibitory mechanism of apoptosis in PTSD. |
| first_indexed | 2024-04-12T00:30:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-04-12T00:30:34Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-05edccad79fe4cc49bd11a7cccb37bbc2022-12-22T03:55:20ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752013-01-0121424210.4103/2277-9175.109757Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorderBehrang AlaniNader MaghsoudiAli KhatibiMahdi NoureddiniFarzad AsefifarJamal ShamsBackground: Post-traumatic stress disorder (PTSD) is a stress-related psychosomatic disorder caused by occurrence of a traumatic event and the hippocampus volume of the patients with Post-traumatic stress disorder decreased. However, the mechanisms that cause such damage are not well-understood. The aim of this study is to detect the expression of apoptosis-related Bax, Bcl-2, Caspase-3 and Insulin-like growth Factor-I proteins in the hippocampus region in the Predatory stress rats. Materials and Methods: A total of 70 male wistar rats were divided into Predatory stress groups of 1d, 2d, 3d, 7d, 14d, 30d and a normal control group (N = 10). Rats were subjected to 5 min of predatory stress and then exposed to the elevated plus-maze (EPM). Serum corticosterone and Insulin-like growth factor-1 level of Hippocampus were measured by ELISA technique. The expression of Bax, Bcl-2, and Caspase-3 were detected by western blotting. Results: Rats spent significantly more time in closed arms of the elevated plus maze (EPM) than control group after exposure to stress. Serum levels of corticosterone significantly increased at 2d-3d. The expression of hippocampal IGF-1 was significantly up-regulated at 1d-2d after stress. Both Bax and the ratio of Bax/Bcl-2 significantly peaked at Predatory stress 2d-14d. Caspase3 was significantly active among 2d-30 compared to the normal control. Conclusion: The activation of caspase-3 in the stress groups indicates that apoptosis may be one of the reasons inducing hippocampus atrophy and play roles in the pathogenesis of PTSD. Increase in hippocampus levels of IGF-1 during early PTSD might be involved in the early molecular inhibitory mechanism of apoptosis in PTSD.http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=42;epage=42;aulast=AlaniApoptosisBaxBcl2caspase-3corticosteroneinsulin-like growth factor-1post-traumatic stress disorderpredator stress |
| spellingShingle | Behrang Alani Nader Maghsoudi Ali Khatibi Mahdi Noureddini Farzad Asefifar Jamal Shams Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder Advanced Biomedical Research Apoptosis Bax Bcl2 caspase-3 corticosterone insulin-like growth factor-1 post-traumatic stress disorder predator stress |
| title | Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder |
| title_full | Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder |
| title_fullStr | Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder |
| title_full_unstemmed | Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder |
| title_short | Study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder |
| title_sort | study of the variations in apoptotic factors in hippocampus of male rats with posttraumatic stress disorder |
| topic | Apoptosis Bax Bcl2 caspase-3 corticosterone insulin-like growth factor-1 post-traumatic stress disorder predator stress |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=42;epage=42;aulast=Alani |
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