Identification of cortactin molecular forms in human urine and their possible diagnostic value
The protein composition of human urine reflects changes in the biochemical and physiological status of an individual and has an essential diagnostic value. Using precipitation/extraction methods we isolated a protein with Mr ~100 kDa in a human urine. MALDI TOF/TOF mass spectrometry identified this...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2021-08-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2021/09/Starykovych_4_21.pdf |
| _version_ | 1827609264772874240 |
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| author | M. Starykovych S. Souchelnytskyi O. Fayura O. Abrahamovych M. Abrahamovych N. Lukavetskyy R. Stoika Y. Kit |
| author_facet | M. Starykovych S. Souchelnytskyi O. Fayura O. Abrahamovych M. Abrahamovych N. Lukavetskyy R. Stoika Y. Kit |
| author_sort | M. Starykovych |
| collection | DOAJ |
| description | The protein composition of human urine reflects changes in the biochemical and physiological status of an individual and has an essential diagnostic value. Using precipitation/extraction methods we isolated a protein with Mr ~100 kDa in a human urine. MALDI TOF/TOF mass spectrometry identified this protein as human Src protein kinase substrate cortactin (UniProtKB/Swiss-Prot: Q14247). Screening of urine samples using Western blotting with specific anti-human cortactin antibodies revealed different proteins immunologically related to cortactin in healthy humans and patients with liver cirrhosis and lung cancer diseases. These data suggest that the level of cortacins isoform in urine might serve as a potential marker for testing acute and systemic diseases. |
| first_indexed | 2024-03-09T07:29:49Z |
| format | Article |
| id | doaj.art-05ee9bb350ed42eaaa06d4e869c3fb94 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T07:29:49Z |
| publishDate | 2021-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-05ee9bb350ed42eaaa06d4e869c3fb942023-12-03T06:27:14ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032021-08-0193410.15407/ubj93.04.103Identification of cortactin molecular forms in human urine and their possible diagnostic valueM. Starykovych0https://orcid.org/0000-0003-0200-7639S. Souchelnytskyi1https://orcid.org/0000-0001-8243-9276O. Fayura2https://orcid.org/0000-0003-1122-9030O. Abrahamovych3https://orcid.org/0000-0001-6862-6809M. Abrahamovych 4https://orcid.org/0000-0002-2827-7170N. Lukavetskyy5R. Stoika 6https://orcid.org/0000-0001-5719-2187Y. Kit7https://orcid.org/0000-0002-5995-4381Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Scoences of UkraineCollege of Medicine, QU Health, Qatar University, Doha, QatarDepartment of Internal Medicine № 1, Danylo Halytsky Lviv National Medical University, UkraineDepartment of Internal Medicine № 1, Danylo Halytsky Lviv National Medical University, UkraineDepartment of Internal Medicine № 1, Danylo Halytsky Lviv National Medical University, UkraineLviv State Oncology Regional Treatment and Diagnostic Center, Lviv, UkraineDepartment of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Scoences of UkraineDepartment of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Scoences of UkraineThe protein composition of human urine reflects changes in the biochemical and physiological status of an individual and has an essential diagnostic value. Using precipitation/extraction methods we isolated a protein with Mr ~100 kDa in a human urine. MALDI TOF/TOF mass spectrometry identified this protein as human Src protein kinase substrate cortactin (UniProtKB/Swiss-Prot: Q14247). Screening of urine samples using Western blotting with specific anti-human cortactin antibodies revealed different proteins immunologically related to cortactin in healthy humans and patients with liver cirrhosis and lung cancer diseases. These data suggest that the level of cortacins isoform in urine might serve as a potential marker for testing acute and systemic diseases.http://ukrbiochemjournal.org/wp-content/uploads/2021/09/Starykovych_4_21.pdfcortactinshuman urineliver cirrhosislung cancer |
| spellingShingle | M. Starykovych S. Souchelnytskyi O. Fayura O. Abrahamovych M. Abrahamovych N. Lukavetskyy R. Stoika Y. Kit Identification of cortactin molecular forms in human urine and their possible diagnostic value The Ukrainian Biochemical Journal cortactins human urine liver cirrhosis lung cancer |
| title | Identification of cortactin molecular forms in human urine and their possible diagnostic value |
| title_full | Identification of cortactin molecular forms in human urine and their possible diagnostic value |
| title_fullStr | Identification of cortactin molecular forms in human urine and their possible diagnostic value |
| title_full_unstemmed | Identification of cortactin molecular forms in human urine and their possible diagnostic value |
| title_short | Identification of cortactin molecular forms in human urine and their possible diagnostic value |
| title_sort | identification of cortactin molecular forms in human urine and their possible diagnostic value |
| topic | cortactins human urine liver cirrhosis lung cancer |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2021/09/Starykovych_4_21.pdf |
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