The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit
Background: Articular cartilage defect can lead to degradation of subchondral bone and osteoarthritis (OA). Objective: To determine the healing effect of transplantation of adipose-derived mesenchymal stem cells (Ad-MSCs) in full-thickness femoral articular cartilage defects in rabbit. Methods: 12...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2015-10-01
|
| Series: | International Journal of Organ Transplantation Medicine |
| Subjects: | |
| Online Access: | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/214 |
| _version_ | 1819059154037243904 |
|---|---|
| author | D Mehrabani M Babazadeh N Tanideh S Zare S Hoseinzadeh S Torabinejad O Koohi-Hosseinabadi |
| author_facet | D Mehrabani M Babazadeh N Tanideh S Zare S Hoseinzadeh S Torabinejad O Koohi-Hosseinabadi |
| author_sort | D Mehrabani |
| collection | DOAJ |
| description | Background: Articular cartilage defect can lead to degradation of subchondral bone and osteoarthritis (OA).
Objective: To determine the healing effect of transplantation of adipose-derived mesenchymal stem cells (Ad-MSCs) in full-thickness femoral articular cartilage defects in rabbit.
Methods: 12 rabbits were equally divided into cell-treated and control groups. In cell-treated group, 2×106 cells of third passage suspended in 1 mL of DMEM was injected into articular defect. The control group just received 1 mL of DMEM. Dulbecco’s modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin and streptomycin and 2 mM L-glutamine were used for cell culture. To induce cartilage defect, 4 mm articular cartilage full-thickness defect was created in the knee. For histological evaluation in each group (H&E, safranin-O and toluidine blue), 3 rabbits were sacrificed 4 weeks and 3 animals, 8 weeks after cell transplantation.
Results: In cell therapy group post-transplantation, no abnormal gross findings were noticed. Neo-formed tissues in cell-treated groups were translucent with a smooth and intact surface and less irregularity. In cell-treated group after 8 weeks post-transplantation, the overall healing score of experimental knees were superior when compared to other groups.
Conclusion: We showed that Ad-MSCs, as an available and non-invasive produced source of cells, could be safely administered in knee osteochondral defects. |
| first_indexed | 2024-12-21T14:06:35Z |
| format | Article |
| id | doaj.art-05efd9ff94594cecb34d520c1e4ade58 |
| institution | Directory Open Access Journal |
| issn | 2008-6482 2008-6490 |
| language | English |
| last_indexed | 2024-12-21T14:06:35Z |
| publishDate | 2015-10-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | International Journal of Organ Transplantation Medicine |
| spelling | doaj.art-05efd9ff94594cecb34d520c1e4ade582022-12-21T19:01:12ZengShiraz University of Medical SciencesInternational Journal of Organ Transplantation Medicine2008-64822008-64902015-10-0164197The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of RabbitD Mehrabani0M Babazadeh1N Tanideh2S Zare3S Hoseinzadeh4S Torabinejad5O Koohi-Hosseinabadi6Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranSchool of Veterinary Medicine, Shiraz University, Shiraz, IranPathology Department, Shiraz University of Medical Sciences, Shiraz, IranLaboratory Animals Center, Shiraz University of Medical Sciences, Shiraz, IranBackground: Articular cartilage defect can lead to degradation of subchondral bone and osteoarthritis (OA). Objective: To determine the healing effect of transplantation of adipose-derived mesenchymal stem cells (Ad-MSCs) in full-thickness femoral articular cartilage defects in rabbit. Methods: 12 rabbits were equally divided into cell-treated and control groups. In cell-treated group, 2×106 cells of third passage suspended in 1 mL of DMEM was injected into articular defect. The control group just received 1 mL of DMEM. Dulbecco’s modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin and streptomycin and 2 mM L-glutamine were used for cell culture. To induce cartilage defect, 4 mm articular cartilage full-thickness defect was created in the knee. For histological evaluation in each group (H&E, safranin-O and toluidine blue), 3 rabbits were sacrificed 4 weeks and 3 animals, 8 weeks after cell transplantation. Results: In cell therapy group post-transplantation, no abnormal gross findings were noticed. Neo-formed tissues in cell-treated groups were translucent with a smooth and intact surface and less irregularity. In cell-treated group after 8 weeks post-transplantation, the overall healing score of experimental knees were superior when compared to other groups. Conclusion: We showed that Ad-MSCs, as an available and non-invasive produced source of cells, could be safely administered in knee osteochondral defects.http://www.ijotm.com/ojs/index.php/IJOTM/article/view/214TransplantationMesenchymal stromal cellsRepairCartilage, articularArticularRabbits |
| spellingShingle | D Mehrabani M Babazadeh N Tanideh S Zare S Hoseinzadeh S Torabinejad O Koohi-Hosseinabadi The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit International Journal of Organ Transplantation Medicine Transplantation Mesenchymal stromal cells Repair Cartilage, articular Articular Rabbits |
| title | The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit |
| title_full | The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit |
| title_fullStr | The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit |
| title_full_unstemmed | The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit |
| title_short | The Healing Effect of Adipose-Derived Mesenchymal Stem Cells in Full-thickness Femoral Articular Cartilage Defects of Rabbit |
| title_sort | healing effect of adipose derived mesenchymal stem cells in full thickness femoral articular cartilage defects of rabbit |
| topic | Transplantation Mesenchymal stromal cells Repair Cartilage, articular Articular Rabbits |
| url | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/214 |
| work_keys_str_mv | AT dmehrabani thehealingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT mbabazadeh thehealingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT ntanideh thehealingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT szare thehealingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT shoseinzadeh thehealingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT storabinejad thehealingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT okoohihosseinabadi thehealingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT dmehrabani healingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT mbabazadeh healingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT ntanideh healingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT szare healingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT shoseinzadeh healingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT storabinejad healingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit AT okoohihosseinabadi healingeffectofadiposederivedmesenchymalstemcellsinfullthicknessfemoralarticularcartilagedefectsofrabbit |