Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean
The constant presence of genetically modified (GM) soybean in conventional seed lots has become a growing problem for international seed trade. In this context, seed companies have prompted the development of routine tests for accurate genetically modified soybean seeds detection. In this study, a...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2017-12-01
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| Series: | Bioscience Journal |
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| Online Access: | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/37236 |
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| author | Joelma Leão-Buchir Gilberto Vinícius Melo Pereira André Luís Lopes da Silva Silvana Alban Maria Carolina Rocha Jossimara Polettini Vanete Thomaz-Soccol Carlos Ricardo Soccol |
| author_facet | Joelma Leão-Buchir Gilberto Vinícius Melo Pereira André Luís Lopes da Silva Silvana Alban Maria Carolina Rocha Jossimara Polettini Vanete Thomaz-Soccol Carlos Ricardo Soccol |
| author_sort | Joelma Leão-Buchir |
| collection | DOAJ |
| description |
The constant presence of genetically modified (GM) soybean in conventional seed lots has become a growing problem for international seed trade. In this context, seed companies have prompted the development of routine tests for accurate genetically modified soybean seeds detection. In this study, a quantitative PCR-based method was standardized in order to detect and quantify mixtures of seeds (i.e. certified seed) or GM grains (i.e. seeds came from field) into samples of non-GM soybean, in a way that soybean lots can be assessed within the standards established by legislation. The method involved the use of p35S-f2/petu-r1 primers targeting CP-4 enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) gene (i.e. that confers herbicide tolerance in Roundup ReadyTM (RR)) for real-time PCR detection and quantification through mericon Quant GMO Detection Assay. The results revealed the method efficiency to detect and quantify the presence of even one soybean seed in batch used for routine evaluation of GM seeds. In addition, it was possible to detect of up to 0.1% of transgenic DNA relative to the soybean grains content. Thus, the sensitive GMO quantitative approach described in this study will provide support in supervising activities, and facilitate the process and control of GM soybean.
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| first_indexed | 2024-12-13T01:44:53Z |
| format | Article |
| id | doaj.art-05f97a4aea67493fafac136d2767b21f |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-13T01:44:53Z |
| publishDate | 2017-12-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-05f97a4aea67493fafac136d2767b21f2022-12-22T00:03:39ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632017-12-01341Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean Joelma Leão-Buchir0Gilberto Vinícius Melo Pereira1André Luís Lopes da Silva2Silvana Alban3Maria Carolina Rocha4Jossimara Polettini5Vanete Thomaz-Soccol6Carlos Ricardo Soccol7Centro de Biotecnologia, Universidade Eduardo Mondlane, Maputo, MoçambiqueDepartamento de Engenharia de Bioprocessos e Biotecnologia; Universidade Federal do Paraná; Curitiba - PR - BrasilDepartamento de Engenharia de Bioprocessos e Biotecnologia; Universidade Federal do Paraná; Curitiba - PR - BrasilDepartamento de Engenharia de Bioprocessos e Biotecnologia; Universidade Federal do Paraná; Curitiba - PR - BrasilDepartamento de Hidráulica e Saneamento, Centro Politécnico, Universidade Federal do Paraná, Curitiba, BrasilUniversidade do Oeste Paulista, UNOESTE, Presidente Prudente, BrasilDepartamento de Engenharia de Bioprocessos e Biotecnologia; Universidade Federal do Paraná; Curitiba - PR - BrasilDepartamento de Engenharia de Bioprocessos e Biotecnologia; Universidade Federal do Paraná; Curitiba - PR - Brasil The constant presence of genetically modified (GM) soybean in conventional seed lots has become a growing problem for international seed trade. In this context, seed companies have prompted the development of routine tests for accurate genetically modified soybean seeds detection. In this study, a quantitative PCR-based method was standardized in order to detect and quantify mixtures of seeds (i.e. certified seed) or GM grains (i.e. seeds came from field) into samples of non-GM soybean, in a way that soybean lots can be assessed within the standards established by legislation. The method involved the use of p35S-f2/petu-r1 primers targeting CP-4 enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) gene (i.e. that confers herbicide tolerance in Roundup ReadyTM (RR)) for real-time PCR detection and quantification through mericon Quant GMO Detection Assay. The results revealed the method efficiency to detect and quantify the presence of even one soybean seed in batch used for routine evaluation of GM seeds. In addition, it was possible to detect of up to 0.1% of transgenic DNA relative to the soybean grains content. Thus, the sensitive GMO quantitative approach described in this study will provide support in supervising activities, and facilitate the process and control of GM soybean. https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/37236Roundup Ready® soybeanGMPReal Time PCRGlycine maxcp4-epsps gene |
| spellingShingle | Joelma Leão-Buchir Gilberto Vinícius Melo Pereira André Luís Lopes da Silva Silvana Alban Maria Carolina Rocha Jossimara Polettini Vanete Thomaz-Soccol Carlos Ricardo Soccol Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean Bioscience Journal Roundup Ready® soybean GMP Real Time PCR Glycine max cp4-epsps gene |
| title | Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean |
| title_full | Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean |
| title_fullStr | Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean |
| title_full_unstemmed | Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean |
| title_short | Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean |
| title_sort | real time pcr for traceability and quantification of genetically modified seeds in lots of non transgenic soybean |
| topic | Roundup Ready® soybean GMP Real Time PCR Glycine max cp4-epsps gene |
| url | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/37236 |
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