The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
Abstract We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these...
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| Format: | Article |
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Nature Portfolio
2018-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-27241-3 |
| _version_ | 1818753904772382720 |
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| author | Muhammad Fariz Zahir Ali Indri Afriani Yasin Takashi Ohta Atsushi Hashizume Atsushi Ido Takayuki Takahashi Chiemi Miura Takeshi Miura |
| author_facet | Muhammad Fariz Zahir Ali Indri Afriani Yasin Takashi Ohta Atsushi Hashizume Atsushi Ido Takayuki Takahashi Chiemi Miura Takeshi Miura |
| author_sort | Muhammad Fariz Zahir Ali |
| collection | DOAJ |
| description | Abstract We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 μg/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity. |
| first_indexed | 2024-12-18T05:14:46Z |
| format | Article |
| id | doaj.art-05f9d1b57dac40689c3b2f3f1a545e49 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T05:14:46Z |
| publishDate | 2018-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-05f9d1b57dac40689c3b2f3f1a545e492022-12-21T21:19:47ZengNature PortfolioScientific Reports2045-23222018-06-01811810.1038/s41598-018-27241-3The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunityMuhammad Fariz Zahir Ali0Indri Afriani Yasin1Takashi Ohta2Atsushi Hashizume3Atsushi Ido4Takayuki Takahashi5Chiemi Miura6Takeshi Miura7Graduate School of Agriculture, Ehime UniversityGraduate School of Agriculture, Ehime UniversitySouth Ehime Fisheries Research Center, Ehime UniversityGraduate School of Agriculture, Ehime UniversitySouth Ehime Fisheries Research Center, Ehime UniversityGraduate School of Agriculture, Ehime UniversityGraduate School of Agriculture, Ehime UniversityGraduate School of Agriculture, Ehime UniversityAbstract We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 μg/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity.https://doi.org/10.1038/s41598-018-27241-3 |
| spellingShingle | Muhammad Fariz Zahir Ali Indri Afriani Yasin Takashi Ohta Atsushi Hashizume Atsushi Ido Takayuki Takahashi Chiemi Miura Takeshi Miura The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity Scientific Reports |
| title | The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity |
| title_full | The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity |
| title_fullStr | The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity |
| title_full_unstemmed | The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity |
| title_short | The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity |
| title_sort | silkrose of bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity |
| url | https://doi.org/10.1038/s41598-018-27241-3 |
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