Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic Pigs
Restrictions on the use of antibiotics in pigs lead to the continuous search for new probiotics serving as an alternative to antibiotics. One of the key parameters for probiotic bacteria selection is the absence of horizontally transmissible resistance genes. The aim of our study was to determine an...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2076-2607/11/1/103 |
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| author | Monika Moravkova Iveta Kostovova Katerina Kavanova Radko Pechar Stanislav Stanek Ales Brychta Michal Zeman Tereza Kubasova |
| author_facet | Monika Moravkova Iveta Kostovova Katerina Kavanova Radko Pechar Stanislav Stanek Ales Brychta Michal Zeman Tereza Kubasova |
| author_sort | Monika Moravkova |
| collection | DOAJ |
| description | Restrictions on the use of antibiotics in pigs lead to the continuous search for new probiotics serving as an alternative to antibiotics. One of the key parameters for probiotic bacteria selection is the absence of horizontally transmissible resistance genes. The aim of our study was to determine antibiotic susceptibility profiles in 28 <i>Lactobacillus amylovorus</i> isolates derived from the digestive tract of wild boars and farm pigs by means of the broth microdilution method and whole genome sequencing (WGS). We revealed genetic resistance determinants and examined sequences flanking resistance genes in these strains. Our findings indicate that <i>L. amylovorus</i> strains from domestic pigs are predominantly resistant to tetracycline, erythromycin and ampicillin. WGS analysis of horizontally transmissible genes revealed only three genetic determinants (<i>tetW</i>, <i>ermB</i> and <i>aadE</i>) of which all <i>tetW</i> and <i>ermB</i> genes were present only in strains derived from domestic pigs. Sequence analysis of coding sequences (CDS) in the neighborhood of the <i>tetW</i> gene revealed the presence of site-specific recombinase (<i>xerC</i>/<i>D</i>), site-specific DNA recombinase (<i>spoIVCA</i>) or DNA-binding transcriptional regulator (<i>xre</i>), usually directly downstream of the <i>tetW</i> gene. In the case of <i>ermB</i>, CDS for omega transcriptional repressor or mobilization protein were detected upstream of the <i>ermB</i> gene. |
| first_indexed | 2024-03-09T11:38:49Z |
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| last_indexed | 2024-03-09T11:38:49Z |
| publishDate | 2022-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-05fbca31190041f98a010008514f36ea2023-11-30T23:36:27ZengMDPI AGMicroorganisms2076-26072022-12-0111110310.3390/microorganisms11010103Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic PigsMonika Moravkova0Iveta Kostovova1Katerina Kavanova2Radko Pechar3Stanislav Stanek4Ales Brychta5Michal Zeman6Tereza Kubasova7Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech RepublicDepartment of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech RepublicDepartment of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech RepublicFood Research Institute Prague, Radiová 1285/7, 102 00 Prague, Czech RepublicMIKROP ČEBÍN a.s., Čebín 416, 664 23 Čebín, Czech RepublicMIKROP ČEBÍN a.s., Čebín 416, 664 23 Čebín, Czech RepublicDepartment of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech RepublicDepartment of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech RepublicRestrictions on the use of antibiotics in pigs lead to the continuous search for new probiotics serving as an alternative to antibiotics. One of the key parameters for probiotic bacteria selection is the absence of horizontally transmissible resistance genes. The aim of our study was to determine antibiotic susceptibility profiles in 28 <i>Lactobacillus amylovorus</i> isolates derived from the digestive tract of wild boars and farm pigs by means of the broth microdilution method and whole genome sequencing (WGS). We revealed genetic resistance determinants and examined sequences flanking resistance genes in these strains. Our findings indicate that <i>L. amylovorus</i> strains from domestic pigs are predominantly resistant to tetracycline, erythromycin and ampicillin. WGS analysis of horizontally transmissible genes revealed only three genetic determinants (<i>tetW</i>, <i>ermB</i> and <i>aadE</i>) of which all <i>tetW</i> and <i>ermB</i> genes were present only in strains derived from domestic pigs. Sequence analysis of coding sequences (CDS) in the neighborhood of the <i>tetW</i> gene revealed the presence of site-specific recombinase (<i>xerC</i>/<i>D</i>), site-specific DNA recombinase (<i>spoIVCA</i>) or DNA-binding transcriptional regulator (<i>xre</i>), usually directly downstream of the <i>tetW</i> gene. In the case of <i>ermB</i>, CDS for omega transcriptional repressor or mobilization protein were detected upstream of the <i>ermB</i> gene.https://www.mdpi.com/2076-2607/11/1/103antibiotic resistance<i>tetW</i><i>ermB</i><i>Lactobacillus amylovorus</i>domestic pigswild boars |
| spellingShingle | Monika Moravkova Iveta Kostovova Katerina Kavanova Radko Pechar Stanislav Stanek Ales Brychta Michal Zeman Tereza Kubasova Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic Pigs Microorganisms antibiotic resistance <i>tetW</i> <i>ermB</i> <i>Lactobacillus amylovorus</i> domestic pigs wild boars |
| title | Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic Pigs |
| title_full | Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic Pigs |
| title_fullStr | Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic Pigs |
| title_full_unstemmed | Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic Pigs |
| title_short | Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in <i>Lactobacillus amylovorus</i> Isolates Derived from Wild Boars and Domestic Pigs |
| title_sort | antibiotic susceptibility resistance gene determinants and corresponding genomic regions in i lactobacillus amylovorus i isolates derived from wild boars and domestic pigs |
| topic | antibiotic resistance <i>tetW</i> <i>ermB</i> <i>Lactobacillus amylovorus</i> domestic pigs wild boars |
| url | https://www.mdpi.com/2076-2607/11/1/103 |
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