Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats
Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder with disrupted uterus structure and function. A positive effect of vitamin D<sub>3</sub> (VD<sub>3</sub>) in female reproduction was observed. Chemerin (RARRES2) and adiponectin (ADIPOQ) are the main adipok...
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MDPI AG
2023-08-01
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| author | Karolina Pich Jesika Rajewska Kinga Kamińska Marcelina Tchurzyk Agata Szlaga Patryk Sambak Anna Błasiak Małgorzata Grzesiak Agnieszka Rak |
| author_facet | Karolina Pich Jesika Rajewska Kinga Kamińska Marcelina Tchurzyk Agata Szlaga Patryk Sambak Anna Błasiak Małgorzata Grzesiak Agnieszka Rak |
| author_sort | Karolina Pich |
| collection | DOAJ |
| description | Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder with disrupted uterus structure and function. A positive effect of vitamin D<sub>3</sub> (VD<sub>3</sub>) in female reproduction was observed. Chemerin (RARRES2) and adiponectin (ADIPOQ) are the main adipokines whose levels are altered in PCOS patients. Therefore, the aim of this study was to investigate the impact of VD<sub>3</sub> supplementation on RARRES2 and ADIPOQ levels in the uterus of PCOS rats. Methods: We analyzed the plasma levels and uterine transcript and protein expression of RARRES2 and ADIPOQ and their receptors (CCRL2, CMKLR1, GPR1, and ADIPOR1 and ADIPOR2, respectively) in rats with letrozole-induced PCOS. Results: In control animals, VD<sub>3</sub> did not change plasma levels of both adipokines, while in PCOS rats supplemented with VD<sub>3</sub>, they returned to control levels. The expression of RARRES2 and all investigated receptors increased in the uterus of VD<sub>3</sub>-treated rats; however, the levels of <i>Rarres2</i> and <i>Gpr1</i> genes remained unchanged. VD<sub>3</sub> supplementation decreased RARRES2, CMKLR1, and GPR1 but increased CCRL2 level to the control value. In the uterus of VD<sub>3</sub>-treated rats, the transcript and protein levels of ADIPOQ and both receptors ADIPOR1 increased. At the same time, VD<sub>3</sub> supplementation induced an increase in <i>Adipoq</i>, <i>Adipor1</i>, and <i>Adipor2</i> gene expression and restored protein levels to control level values. Conclusions: our findings indicate a new mechanism of VD<sub>3</sub> action in the uterine physiology of PCOS rats. |
| first_indexed | 2024-03-11T00:02:58Z |
| format | Article |
| id | doaj.art-05fcfe0e64b2412ba1c45510675a458a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T00:02:58Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-05fcfe0e64b2412ba1c45510675a458a2023-11-19T00:36:17ZengMDPI AGCells2073-44092023-08-011216202610.3390/cells12162026Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome RatsKarolina Pich0Jesika Rajewska1Kinga Kamińska2Marcelina Tchurzyk3Agata Szlaga4Patryk Sambak5Anna Błasiak6Małgorzata Grzesiak7Agnieszka Rak8Laboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, PolandLaboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, PolandDoctoral School of Exact and Natural Sciences, Jagiellonian University, 30-348 Kraków, PolandDepartment of Endocrinology, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, PolandDepartment of Neurophysiology and Chronobiology, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, PolandDoctoral School of Exact and Natural Sciences, Jagiellonian University, 30-348 Kraków, PolandDepartment of Neurophysiology and Chronobiology, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, PolandDepartment of Endocrinology, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, PolandLaboratory of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, PolandBackground: Polycystic ovary syndrome (PCOS) is an endocrine disorder with disrupted uterus structure and function. A positive effect of vitamin D<sub>3</sub> (VD<sub>3</sub>) in female reproduction was observed. Chemerin (RARRES2) and adiponectin (ADIPOQ) are the main adipokines whose levels are altered in PCOS patients. Therefore, the aim of this study was to investigate the impact of VD<sub>3</sub> supplementation on RARRES2 and ADIPOQ levels in the uterus of PCOS rats. Methods: We analyzed the plasma levels and uterine transcript and protein expression of RARRES2 and ADIPOQ and their receptors (CCRL2, CMKLR1, GPR1, and ADIPOR1 and ADIPOR2, respectively) in rats with letrozole-induced PCOS. Results: In control animals, VD<sub>3</sub> did not change plasma levels of both adipokines, while in PCOS rats supplemented with VD<sub>3</sub>, they returned to control levels. The expression of RARRES2 and all investigated receptors increased in the uterus of VD<sub>3</sub>-treated rats; however, the levels of <i>Rarres2</i> and <i>Gpr1</i> genes remained unchanged. VD<sub>3</sub> supplementation decreased RARRES2, CMKLR1, and GPR1 but increased CCRL2 level to the control value. In the uterus of VD<sub>3</sub>-treated rats, the transcript and protein levels of ADIPOQ and both receptors ADIPOR1 increased. At the same time, VD<sub>3</sub> supplementation induced an increase in <i>Adipoq</i>, <i>Adipor1</i>, and <i>Adipor2</i> gene expression and restored protein levels to control level values. Conclusions: our findings indicate a new mechanism of VD<sub>3</sub> action in the uterine physiology of PCOS rats.https://www.mdpi.com/2073-4409/12/16/2026vitamin D<sub>3</sub>chemerinadiponectinPCOSuterusrats |
| spellingShingle | Karolina Pich Jesika Rajewska Kinga Kamińska Marcelina Tchurzyk Agata Szlaga Patryk Sambak Anna Błasiak Małgorzata Grzesiak Agnieszka Rak Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats Cells vitamin D<sub>3</sub> chemerin adiponectin PCOS uterus rats |
| title | Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats |
| title_full | Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats |
| title_fullStr | Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats |
| title_full_unstemmed | Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats |
| title_short | Effect of Vitamin D<sub>3</sub> on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats |
| title_sort | effect of vitamin d sub 3 sub on chemerin and adiponectin levels in uterus of polycystic ovary syndrome rats |
| topic | vitamin D<sub>3</sub> chemerin adiponectin PCOS uterus rats |
| url | https://www.mdpi.com/2073-4409/12/16/2026 |
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