Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation
OBJECTIVES: Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived st...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier España
2012-01-01
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| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000200003 |
| _version_ | 1828464825684983808 |
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| author | Adila A Hamid Ruszymah Bt Hj Idrus Aminuddin Bin Saim Somasumdaram Sathappan Kien-Hui Chua |
| author_facet | Adila A Hamid Ruszymah Bt Hj Idrus Aminuddin Bin Saim Somasumdaram Sathappan Kien-Hui Chua |
| author_sort | Adila A Hamid |
| collection | DOAJ |
| description | OBJECTIVES: Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction. MATERIALS AND METHODS: Human adipose-derived stem cells at passage 4 were evaluated by flow cytometry to examine the expression of surface markers. These adipose-derived stem cells were tested for adipogenic and osteogenic differentiation capacity. Ribonucleic acid was extracted from the cells for quantitative polymerase chain reaction analysis to determine the expression levels of chondrogenic genes after chondrogenic induction. RESULTS: Human adipose-derived stem cells were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen and successfully differentiated into adipogenic and osteogenic lineages. The human adipose-derived stem cells aggregated and formed a dense matrix after chondrogenic induction. The expression of chondrogenic genes (collagen type II, aggrecan core protein, collagen type XI, COMP, and ELASTIN) was significantly higher after the first week of induction. However, a significantly elevated expression of collagen type X was observed after three weeks of chondrogenic induction. CONCLUSION: Human adipose-derived stem cells retain stem cell characteristics after expansion in culture to passage 4 and serve as a feasible source of cells for cartilage regeneration. Chondrogenesis in human adiposederived stem cells was most prominent after one week of chondrogenic induction. |
| first_indexed | 2024-12-11T03:20:03Z |
| format | Article |
| id | doaj.art-05fd5d759fcd4cd79214c55b655e6616 |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-12-11T03:20:03Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-05fd5d759fcd4cd79214c55b655e66162022-12-22T01:22:38ZengElsevier EspañaClinics1807-59321980-53222012-01-0167209910610.6061/clinics/2012(02)03Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiationAdila A HamidRuszymah Bt Hj IdrusAminuddin Bin SaimSomasumdaram SathappanKien-Hui ChuaOBJECTIVES: Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction. MATERIALS AND METHODS: Human adipose-derived stem cells at passage 4 were evaluated by flow cytometry to examine the expression of surface markers. These adipose-derived stem cells were tested for adipogenic and osteogenic differentiation capacity. Ribonucleic acid was extracted from the cells for quantitative polymerase chain reaction analysis to determine the expression levels of chondrogenic genes after chondrogenic induction. RESULTS: Human adipose-derived stem cells were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen and successfully differentiated into adipogenic and osteogenic lineages. The human adipose-derived stem cells aggregated and formed a dense matrix after chondrogenic induction. The expression of chondrogenic genes (collagen type II, aggrecan core protein, collagen type XI, COMP, and ELASTIN) was significantly higher after the first week of induction. However, a significantly elevated expression of collagen type X was observed after three weeks of chondrogenic induction. CONCLUSION: Human adipose-derived stem cells retain stem cell characteristics after expansion in culture to passage 4 and serve as a feasible source of cells for cartilage regeneration. Chondrogenesis in human adiposederived stem cells was most prominent after one week of chondrogenic induction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000200003ChondrogenesisCell-based therapyCartilageChondrocytesAdiposeStem cellsDifferentiation |
| spellingShingle | Adila A Hamid Ruszymah Bt Hj Idrus Aminuddin Bin Saim Somasumdaram Sathappan Kien-Hui Chua Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation Clinics Chondrogenesis Cell-based therapy Cartilage Chondrocytes Adipose Stem cells Differentiation |
| title | Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation |
| title_full | Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation |
| title_fullStr | Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation |
| title_full_unstemmed | Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation |
| title_short | Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation |
| title_sort | characterization of human adipose derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation |
| topic | Chondrogenesis Cell-based therapy Cartilage Chondrocytes Adipose Stem cells Differentiation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000200003 |
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