Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC)
Retention mechanisms in HILIC have been investigated and reported in literature. However, the current understanding of retention mechanisms is qualitative and lacks quantitative details. Previously, mechanism elucidation was based on indirect evidence, and unambiguous assignment of retention mechani...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/18/6459 |
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| author | Yong Guo Dominik Baran |
| author_facet | Yong Guo Dominik Baran |
| author_sort | Yong Guo |
| collection | DOAJ |
| description | Retention mechanisms in HILIC have been investigated and reported in literature. However, the current understanding of retention mechanisms is qualitative and lacks quantitative details. Previously, mechanism elucidation was based on indirect evidence, and unambiguous assignment of retention mechanisms has not been reported based on direct data. This study aims to quantitatively determine the contributions of two major retention mechanisms in HILIC, hydrophilic partitioning and surface adsorption to the overall retention of neutral compounds. Using the methodologies we developed previously, the phase ratio for adsorbed water layer and distribution coefficients were measured and used to calculate the retention factors contributed by hydrophilic partitioning. The methodology allows the determination of the contribution of surface adsorption simultaneously. The evaluation of five test compounds demonstrates that the retention may be controlled by hydrophilic partitioning, surface adsorption or both depending on compound characteristics. Quantitative assessment of retention mechanisms also makes it possible to better understand the effect of acetonitrile on retention in HILIC. |
| first_indexed | 2024-03-10T22:24:45Z |
| format | Article |
| id | doaj.art-0602347de001403baf5a3497fc343574 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T22:24:45Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-0602347de001403baf5a3497fc3435742023-11-19T12:07:40ZengMDPI AGMolecules1420-30492023-09-012818645910.3390/molecules28186459Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC)Yong Guo0Dominik Baran1School of Pharmacy and Health Sciences, Fairleigh Dickinson University, Florham Park, NJ 07932, USASchool of Pharmacy and Health Sciences, Fairleigh Dickinson University, Florham Park, NJ 07932, USARetention mechanisms in HILIC have been investigated and reported in literature. However, the current understanding of retention mechanisms is qualitative and lacks quantitative details. Previously, mechanism elucidation was based on indirect evidence, and unambiguous assignment of retention mechanisms has not been reported based on direct data. This study aims to quantitatively determine the contributions of two major retention mechanisms in HILIC, hydrophilic partitioning and surface adsorption to the overall retention of neutral compounds. Using the methodologies we developed previously, the phase ratio for adsorbed water layer and distribution coefficients were measured and used to calculate the retention factors contributed by hydrophilic partitioning. The methodology allows the determination of the contribution of surface adsorption simultaneously. The evaluation of five test compounds demonstrates that the retention may be controlled by hydrophilic partitioning, surface adsorption or both depending on compound characteristics. Quantitative assessment of retention mechanisms also makes it possible to better understand the effect of acetonitrile on retention in HILIC.https://www.mdpi.com/1420-3049/28/18/6459retention mechanismpartitioningadsorptionphase ratioquantitativeHILIC |
| spellingShingle | Yong Guo Dominik Baran Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC) Molecules retention mechanism partitioning adsorption phase ratio quantitative HILIC |
| title | Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC) |
| title_full | Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC) |
| title_fullStr | Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC) |
| title_full_unstemmed | Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC) |
| title_short | Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC) |
| title_sort | hydrophilic partitioning or surface adsorption a quantitative assessment of retention mechanisms for hydrophilic interaction chromatography hilic |
| topic | retention mechanism partitioning adsorption phase ratio quantitative HILIC |
| url | https://www.mdpi.com/1420-3049/28/18/6459 |
| work_keys_str_mv | AT yongguo hydrophilicpartitioningorsurfaceadsorptionaquantitativeassessmentofretentionmechanismsforhydrophilicinteractionchromatographyhilic AT dominikbaran hydrophilicpartitioningorsurfaceadsorptionaquantitativeassessmentofretentionmechanismsforhydrophilicinteractionchromatographyhilic |