Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice
Although Staphylococcus aureus is part of the normal body flora, heavy usage of antibiotics has resulted in the emergence of methicillin-resistant strains (MRSA). MRSA can form biofilms and cause indwelling foreign body infections, bacteremia, soft tissue infections, endocarditis, and osteomyelitis....
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MDPI AG
2016-06-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/7/198 |
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| author | Takuto Oyama Motoyasu Miyazaki Michinobu Yoshimura Tohru Takata Hiroyuki Ohjimi Shiro Jimi |
| author_facet | Takuto Oyama Motoyasu Miyazaki Michinobu Yoshimura Tohru Takata Hiroyuki Ohjimi Shiro Jimi |
| author_sort | Takuto Oyama |
| collection | DOAJ |
| description | Although Staphylococcus aureus is part of the normal body flora, heavy usage of antibiotics has resulted in the emergence of methicillin-resistant strains (MRSA). MRSA can form biofilms and cause indwelling foreign body infections, bacteremia, soft tissue infections, endocarditis, and osteomyelitis. Using an in vitro assay, we screened 173 clinical blood isolates of MRSA and selected 20 high-biofilm formers (H-BF) and low-biofilm formers (L-BF). These were intravenously administered to mice and the general condition of mice, the distribution of bacteria, and biofilm in the liver, lung, spleen, and kidney were investigated. MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are associated with intracellular biofilm. After 24 h, the general condition of the mice worsened significantly in the H-BF group. In the liver, bacterial deposition and aggregation and the biofilm-forming spot number were all significantly greater for H-BF group than for L-BF. CFU analysis revealed that bacteria in the H-BF group survived for long periods in the liver. These results indicate that the biofilm-forming ability of MRSA is a crucial factor for intracellular persistence, which could lead to chronic infections. |
| first_indexed | 2024-04-11T14:05:28Z |
| format | Article |
| id | doaj.art-0603d3992f9947b2878be7f28bc44878 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T14:05:28Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-0603d3992f9947b2878be7f28bc448782022-12-22T04:19:53ZengMDPI AGToxins2072-66512016-06-018719810.3390/toxins8070198toxins8070198Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in MiceTakuto Oyama0Motoyasu Miyazaki1Michinobu Yoshimura2Tohru Takata3Hiroyuki Ohjimi4Shiro Jimi5Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Pharmacy, Fukuoka University Chikushi Hospital, Chikusino 818-8502, JapanDepartment of Medical Oncology, Hematology, and Infectious Diseases, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Medical Oncology, Hematology, and Infectious Diseases, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, JapanCentral Laboratory for Pathology and Morphology, Department of Pathology, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, JapanAlthough Staphylococcus aureus is part of the normal body flora, heavy usage of antibiotics has resulted in the emergence of methicillin-resistant strains (MRSA). MRSA can form biofilms and cause indwelling foreign body infections, bacteremia, soft tissue infections, endocarditis, and osteomyelitis. Using an in vitro assay, we screened 173 clinical blood isolates of MRSA and selected 20 high-biofilm formers (H-BF) and low-biofilm formers (L-BF). These were intravenously administered to mice and the general condition of mice, the distribution of bacteria, and biofilm in the liver, lung, spleen, and kidney were investigated. MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are associated with intracellular biofilm. After 24 h, the general condition of the mice worsened significantly in the H-BF group. In the liver, bacterial deposition and aggregation and the biofilm-forming spot number were all significantly greater for H-BF group than for L-BF. CFU analysis revealed that bacteria in the H-BF group survived for long periods in the liver. These results indicate that the biofilm-forming ability of MRSA is a crucial factor for intracellular persistence, which could lead to chronic infections.http://www.mdpi.com/2072-6651/8/7/198MRSAvirulencebiofilmintracellular persistenceKupffer cellsmice |
| spellingShingle | Takuto Oyama Motoyasu Miyazaki Michinobu Yoshimura Tohru Takata Hiroyuki Ohjimi Shiro Jimi Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice Toxins MRSA virulence biofilm intracellular persistence Kupffer cells mice |
| title | Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice |
| title_full | Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice |
| title_fullStr | Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice |
| title_full_unstemmed | Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice |
| title_short | Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice |
| title_sort | biofilm forming methicillin resistant staphylococcus aureus survive in kupffer cells and exhibit high virulence in mice |
| topic | MRSA virulence biofilm intracellular persistence Kupffer cells mice |
| url | http://www.mdpi.com/2072-6651/8/7/198 |
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