High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium
Nannochloropsis salina was grown on a mixture of standard growth media and pre-gasified industrial process water representing effluent from a local biogas plant. The study aimed to investigate the effects of enriched growth media and cultivation time on nutritional composition of Nannochloropsis sal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-07-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/14/8/144 |
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| author | Hamed Safafar Michael Z. Hass Per Møller Susan L. Holdt Charlotte Jacobsen |
| author_facet | Hamed Safafar Michael Z. Hass Per Møller Susan L. Holdt Charlotte Jacobsen |
| author_sort | Hamed Safafar |
| collection | DOAJ |
| description | Nannochloropsis salina was grown on a mixture of standard growth media and pre-gasified industrial process water representing effluent from a local biogas plant. The study aimed to investigate the effects of enriched growth media and cultivation time on nutritional composition of Nannochloropsis salina biomass, with a focus on eicosapentaenoic acid (EPA). Variations in fatty acid composition, lipids, protein, amino acids, tocopherols and pigments were studied and results compared to algae cultivated on F/2 media as reference. Mixed growth media and process water enhanced the nutritional quality of Nannochloropsis salina in laboratory scale when compared to algae cultivated in standard F/2 medium. Data from laboratory scale translated to the large scale using a 4000 L flat panel photo-bioreactor system. The algae growth rate in winter conditions in Denmark was slow, but results revealed that large-scale cultivation of Nannochloropsis salina at these conditions could improve the nutritional properties such as EPA, tocopherol, protein and carotenoids compared to laboratory-scale cultivated microalgae. EPA reached 44.2% ± 2.30% of total fatty acids, and α-tocopherol reached 431 ± 28 µg/g of biomass dry weight after 21 days of cultivation. Variations in chemical compositions of Nannochloropsis salina were studied during the course of cultivation. Nannochloropsis salina can be presented as a good candidate for winter time cultivation in Denmark. The resulting biomass is a rich source of EPA and also a good source of protein (amino acids), tocopherols and carotenoids for potential use in aquaculture feed industry. |
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| id | doaj.art-0603fb18f0c54a3587497bfa84cfc5b0 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:08:34Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-0603fb18f0c54a3587497bfa84cfc5b02022-12-22T04:00:37ZengMDPI AGMarine Drugs1660-33972016-07-0114814410.3390/md14080144md14080144High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth MediumHamed Safafar0Michael Z. Hass1Per Møller2Susan L. Holdt3Charlotte Jacobsen4Technical University of Denmark, National Food Institute (DTU Food), Søltofts Plads, Building 221, Kongens Lyngby 2800, DenmarkKalundborg Municipality, Department Development, Torvet 3A, Kalundborg 4400, DenmarkKalundborg Municipality, Department Development, Torvet 3A, Kalundborg 4400, DenmarkTechnical University of Denmark, National Food Institute (DTU Food), Søltofts Plads, Building 221, Kongens Lyngby 2800, DenmarkTechnical University of Denmark, National Food Institute (DTU Food), Søltofts Plads, Building 221, Kongens Lyngby 2800, DenmarkNannochloropsis salina was grown on a mixture of standard growth media and pre-gasified industrial process water representing effluent from a local biogas plant. The study aimed to investigate the effects of enriched growth media and cultivation time on nutritional composition of Nannochloropsis salina biomass, with a focus on eicosapentaenoic acid (EPA). Variations in fatty acid composition, lipids, protein, amino acids, tocopherols and pigments were studied and results compared to algae cultivated on F/2 media as reference. Mixed growth media and process water enhanced the nutritional quality of Nannochloropsis salina in laboratory scale when compared to algae cultivated in standard F/2 medium. Data from laboratory scale translated to the large scale using a 4000 L flat panel photo-bioreactor system. The algae growth rate in winter conditions in Denmark was slow, but results revealed that large-scale cultivation of Nannochloropsis salina at these conditions could improve the nutritional properties such as EPA, tocopherol, protein and carotenoids compared to laboratory-scale cultivated microalgae. EPA reached 44.2% ± 2.30% of total fatty acids, and α-tocopherol reached 431 ± 28 µg/g of biomass dry weight after 21 days of cultivation. Variations in chemical compositions of Nannochloropsis salina were studied during the course of cultivation. Nannochloropsis salina can be presented as a good candidate for winter time cultivation in Denmark. The resulting biomass is a rich source of EPA and also a good source of protein (amino acids), tocopherols and carotenoids for potential use in aquaculture feed industry.http://www.mdpi.com/1660-3397/14/8/144EPANannochloropsis salinaindustrial process watermicroalgaecarotenoidsamino acidtocopherollarge scaleflat panel photo-bioreactor |
| spellingShingle | Hamed Safafar Michael Z. Hass Per Møller Susan L. Holdt Charlotte Jacobsen High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium Marine Drugs EPA Nannochloropsis salina industrial process water microalgae carotenoids amino acid tocopherol large scale flat panel photo-bioreactor |
| title | High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium |
| title_full | High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium |
| title_fullStr | High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium |
| title_full_unstemmed | High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium |
| title_short | High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium |
| title_sort | high epa biomass from nannochloropsis salina cultivated in a flat panel photo bioreactor on a process water enriched growth medium |
| topic | EPA Nannochloropsis salina industrial process water microalgae carotenoids amino acid tocopherol large scale flat panel photo-bioreactor |
| url | http://www.mdpi.com/1660-3397/14/8/144 |
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