An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation
Although rats are preferred over mice as an animal model, transgenic animals are generated predominantly using mouse embryos. There are limitations in the generation of transgenic rat by embryo manipulation. Unlike mouse embryos, most of the rat embryos do not survive after male pronuclear DNA injec...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-01-01
|
| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253117301208 |
| _version_ | 1811196678514933760 |
|---|---|
| author | Bhola Shankar Pradhan Subeer S. Majumdar |
| author_facet | Bhola Shankar Pradhan Subeer S. Majumdar |
| author_sort | Bhola Shankar Pradhan |
| collection | DOAJ |
| description | Although rats are preferred over mice as an animal model, transgenic animals are generated predominantly using mouse embryos. There are limitations in the generation of transgenic rat by embryo manipulation. Unlike mouse embryos, most of the rat embryos do not survive after male pronuclear DNA injection which reduces the efficiency of generation of transgenic rat by this method. More importantly, this method requires hundreds of eggs collected by killing several females for insertion of transgene to generate transgenic rat. To this end, we developed a noninvasive and deathless technique for generation of transgenic rats by integrating transgene into the genome of the spermatogonial cells by testicular injection of DNA followed by electroporation. After standardization of this technique using EGFP as a transgene, a transgenic disease model displaying alpha thalassemia was successfully generated using rats. This efficient method will ease the generation of transgenic rats without killing the lives of rats while simultaneously reducing the number of rats used for generation of transgenic animal. |
| first_indexed | 2024-04-12T01:02:59Z |
| format | Article |
| id | doaj.art-060422ac869a44f48e95557d21848443 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-04-12T01:02:59Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-060422ac869a44f48e95557d218484432022-12-22T03:54:21ZengElsevierMolecular Therapy: Nucleic Acids2162-25312016-01-015C10.1038/mtna.2016.9An Efficient Method for Generation of Transgenic Rats Avoiding Embryo ManipulationBhola Shankar Pradhan0Subeer S. Majumdar1Cellular Endocrinology Laboratory, National Institute of Immunology, New Delhi, IndiaCellular Endocrinology Laboratory, National Institute of Immunology, New Delhi, IndiaAlthough rats are preferred over mice as an animal model, transgenic animals are generated predominantly using mouse embryos. There are limitations in the generation of transgenic rat by embryo manipulation. Unlike mouse embryos, most of the rat embryos do not survive after male pronuclear DNA injection which reduces the efficiency of generation of transgenic rat by this method. More importantly, this method requires hundreds of eggs collected by killing several females for insertion of transgene to generate transgenic rat. To this end, we developed a noninvasive and deathless technique for generation of transgenic rats by integrating transgene into the genome of the spermatogonial cells by testicular injection of DNA followed by electroporation. After standardization of this technique using EGFP as a transgene, a transgenic disease model displaying alpha thalassemia was successfully generated using rats. This efficient method will ease the generation of transgenic rats without killing the lives of rats while simultaneously reducing the number of rats used for generation of transgenic animal.http://www.sciencedirect.com/science/article/pii/S2162253117301208electroporationembryospermatogonial germ cellthalassemiatransgenic rat |
| spellingShingle | Bhola Shankar Pradhan Subeer S. Majumdar An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation Molecular Therapy: Nucleic Acids electroporation embryo spermatogonial germ cell thalassemia transgenic rat |
| title | An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation |
| title_full | An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation |
| title_fullStr | An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation |
| title_full_unstemmed | An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation |
| title_short | An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation |
| title_sort | efficient method for generation of transgenic rats avoiding embryo manipulation |
| topic | electroporation embryo spermatogonial germ cell thalassemia transgenic rat |
| url | http://www.sciencedirect.com/science/article/pii/S2162253117301208 |
| work_keys_str_mv | AT bholashankarpradhan anefficientmethodforgenerationoftransgenicratsavoidingembryomanipulation AT subeersmajumdar anefficientmethodforgenerationoftransgenicratsavoidingembryomanipulation AT bholashankarpradhan efficientmethodforgenerationoftransgenicratsavoidingembryomanipulation AT subeersmajumdar efficientmethodforgenerationoftransgenicratsavoidingembryomanipulation |