Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques
Abstract Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study was limited. The present study explored...
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-53880-w |
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| author | Vorthon Sawaswong Prangwalai Chanchaem Pavit Klomkliew Suwatchareeporn Rotcheewaphan Suthirote Meesawat Taratorn Kemthong Mutchamon Kaewparuehaschai Kirana Noradechanon Monya Ekatat Reka Kanitpun Prapaporn Srilohasin Saradee Warit Angkana Chaiprasert Suchinda Malaivijitnond Sunchai Payungporn |
| author_facet | Vorthon Sawaswong Prangwalai Chanchaem Pavit Klomkliew Suwatchareeporn Rotcheewaphan Suthirote Meesawat Taratorn Kemthong Mutchamon Kaewparuehaschai Kirana Noradechanon Monya Ekatat Reka Kanitpun Prapaporn Srilohasin Saradee Warit Angkana Chaiprasert Suchinda Malaivijitnond Sunchai Payungporn |
| author_sort | Vorthon Sawaswong |
| collection | DOAJ |
| description | Abstract Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study was limited. The present study explored the association between gut-pharyngeal microbiome and TB stages in cynomolgus macaques using the full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. The total of 71 macaques was divided into TB (−) control, TB (+) latent and TB (+) active groups. The differential abundance analysis showed that Haemophilus hemolyticus was decreased, while Prevotella species were increased in the pharyngeal microbiome of TB (+) macaques. In addition, Eubacterium coprostanoligenes in the gut was enriched in TB (+) macaques. Alteration of these bacteria might affect immune regulation and TB severity, but details of mechanisms should be further explored and validated. In summary, microbiota may be associated with host immune regulation and affect TB progression. The findings suggested the potential mechanisms of host-microbes interaction, which may improve the understanding of the role of microbiota and help develop therapeutics for TB in the future. |
| first_indexed | 2024-03-07T15:01:01Z |
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| id | doaj.art-06047d4164bf49c2a3a000df7c9e037c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:01:01Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-06047d4164bf49c2a3a000df7c9e037c2024-03-05T19:07:41ZengNature PortfolioScientific Reports2045-23222024-02-0114111510.1038/s41598-024-53880-wFull-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaquesVorthon Sawaswong0Prangwalai Chanchaem1Pavit Klomkliew2Suwatchareeporn Rotcheewaphan3Suthirote Meesawat4Taratorn Kemthong5Mutchamon Kaewparuehaschai6Kirana Noradechanon7Monya Ekatat8Reka Kanitpun9Prapaporn Srilohasin10Saradee Warit11Angkana Chaiprasert12Suchinda Malaivijitnond13Sunchai Payungporn14Department of Biochemistry, Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn UniversityDepartment of Biochemistry, Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn UniversityDepartment of Biochemistry, Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn UniversityDepartment of Biochemistry, Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn UniversityNational Primate Research Center of Thailand, Chulalongkorn UniversityNational Primate Research Center of Thailand, Chulalongkorn UniversityWildlife Conservation Office, Department of National Parks Wildlife and Plant ConservationWildlife Conservation Office, Department of National Parks Wildlife and Plant ConservationNational Institute of Animal Health (NIAH)National Institute of Animal Health (NIAH)Office for Research, Faculty of Medicine Siriraj Hospital, Mahidol UniversityIndustrial Tuberculosis Team, Industrial Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyOffice for Research, Faculty of Medicine Siriraj Hospital, Mahidol UniversityNational Primate Research Center of Thailand, Chulalongkorn UniversityDepartment of Biochemistry, Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn UniversityAbstract Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study was limited. The present study explored the association between gut-pharyngeal microbiome and TB stages in cynomolgus macaques using the full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. The total of 71 macaques was divided into TB (−) control, TB (+) latent and TB (+) active groups. The differential abundance analysis showed that Haemophilus hemolyticus was decreased, while Prevotella species were increased in the pharyngeal microbiome of TB (+) macaques. In addition, Eubacterium coprostanoligenes in the gut was enriched in TB (+) macaques. Alteration of these bacteria might affect immune regulation and TB severity, but details of mechanisms should be further explored and validated. In summary, microbiota may be associated with host immune regulation and affect TB progression. The findings suggested the potential mechanisms of host-microbes interaction, which may improve the understanding of the role of microbiota and help develop therapeutics for TB in the future.https://doi.org/10.1038/s41598-024-53880-wTuberculosisCynomolgus macaqueGut-pharyngeal microbiomeFull-length 16S sequencing |
| spellingShingle | Vorthon Sawaswong Prangwalai Chanchaem Pavit Klomkliew Suwatchareeporn Rotcheewaphan Suthirote Meesawat Taratorn Kemthong Mutchamon Kaewparuehaschai Kirana Noradechanon Monya Ekatat Reka Kanitpun Prapaporn Srilohasin Saradee Warit Angkana Chaiprasert Suchinda Malaivijitnond Sunchai Payungporn Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques Scientific Reports Tuberculosis Cynomolgus macaque Gut-pharyngeal microbiome Full-length 16S sequencing |
| title | Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques |
| title_full | Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques |
| title_fullStr | Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques |
| title_full_unstemmed | Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques |
| title_short | Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques |
| title_sort | full length 16s rdna sequencing based on oxford nanopore technologies revealed the association between gut pharyngeal microbiota and tuberculosis in cynomolgus macaques |
| topic | Tuberculosis Cynomolgus macaque Gut-pharyngeal microbiome Full-length 16S sequencing |
| url | https://doi.org/10.1038/s41598-024-53880-w |
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