A CRISPR-Cas12a—Based platform for ultrasensitive, rapid, and highly specific detection of Mycoplasma pneumonia in clinical application

A CRISPR-Cas12a—Based platform for ultrasensitive, rapid, and highly specific detection of Mycoplasma pneumonia in clinical application

Mycoplasma pneumoniae (MP), which is responsible for a majority of community-acquired pneumonia (CAP) in children, has been largely underestimated. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a novel detection platf...

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Bibliographic Details
Main Authors: Nan Jia, Juan Zhou, Fei Xiao, Baoying Zheng, Xiaolan Huang, Chunrong Sun, Jin Fu, Zheng Xu, Min Chen, Yi Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-01-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Mycoplasma pneumoniae
multiple cross displacement amplification
CRISPR
Cas12a
community-acquired pneumonia
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1022066/full

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