Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1
The properties of human plasma very low density lipoproteins (VLDL), low density lipoproteins (LDL), and their extracted lipids were compared using calorimetric, X-ray scattering, and polarizing microscopy techniques. Intact LDL, and cholesterol esters isolated from LDL and VLDL each undergo reversi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1977-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520416955 |
| _version_ | 1818459733403631616 |
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| author | R J Deckelbaum A R Tall D M Small |
| author_facet | R J Deckelbaum A R Tall D M Small |
| author_sort | R J Deckelbaum |
| collection | DOAJ |
| description | The properties of human plasma very low density lipoproteins (VLDL), low density lipoproteins (LDL), and their extracted lipids were compared using calorimetric, X-ray scattering, and polarizing microscopy techniques. Intact LDL, and cholesterol esters isolated from LDL and VLDL each undergo reversible changes in their physical state around body temperature. These transitions are associated with ordered liquid crystalline to liquid phase changes of the cholesterol esters. In contrast to LDL, VLDL has no reversible transitions and shows no evidence of ordered liquid crystalline structures between 10 and 45 ° C. Therefore, unlike LDL, VLDL does not contain a separate cholesterol ester region capable of undergoing cooperative melting. Solubility studies at 37 ° C of cholesterol esters and triglyceride isolated from VLDL show that even at a weight ratio of 1:1, which greatly exceeds the relative amount of cholesterol esters in VLDL, cholesterol ester is completely soluble in triglyceride. Thus, the cholesterol ester in VLDL is not sequestered in a separate domain within VLDL, but is dissolved in the liquid core of the particle. |
| first_indexed | 2024-12-14T23:19:03Z |
| format | Article |
| id | doaj.art-0607e033f2694e1c89a27eaacc6cd3f9 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T23:19:03Z |
| publishDate | 1977-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-0607e033f2694e1c89a27eaacc6cd3f92022-12-21T22:44:01ZengElsevierJournal of Lipid Research0022-22751977-03-01182164168Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1R J Deckelbaum0A R Tall1D M Small2Department of Medicine, Boston University School of Medicine, Boston, MA 021 18; Department of Pediatrics, Hadassah University Hospital, Jerusalem, Israel.Department of Medicine, Boston University School of Medicine, Boston, MA 021 18Department of Medicine, Boston University School of Medicine, Boston, MA 021 18The properties of human plasma very low density lipoproteins (VLDL), low density lipoproteins (LDL), and their extracted lipids were compared using calorimetric, X-ray scattering, and polarizing microscopy techniques. Intact LDL, and cholesterol esters isolated from LDL and VLDL each undergo reversible changes in their physical state around body temperature. These transitions are associated with ordered liquid crystalline to liquid phase changes of the cholesterol esters. In contrast to LDL, VLDL has no reversible transitions and shows no evidence of ordered liquid crystalline structures between 10 and 45 ° C. Therefore, unlike LDL, VLDL does not contain a separate cholesterol ester region capable of undergoing cooperative melting. Solubility studies at 37 ° C of cholesterol esters and triglyceride isolated from VLDL show that even at a weight ratio of 1:1, which greatly exceeds the relative amount of cholesterol esters in VLDL, cholesterol ester is completely soluble in triglyceride. Thus, the cholesterol ester in VLDL is not sequestered in a separate domain within VLDL, but is dissolved in the liquid core of the particle.http://www.sciencedirect.com/science/article/pii/S0022227520416955low density lipoproteinlipoprotein structurelipid phase behaviorscanning calorimetry |
| spellingShingle | R J Deckelbaum A R Tall D M Small Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1 Journal of Lipid Research low density lipoprotein lipoprotein structure lipid phase behavior scanning calorimetry |
| title | Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1 |
| title_full | Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1 |
| title_fullStr | Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1 |
| title_full_unstemmed | Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1 |
| title_short | Interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1 |
| title_sort | interaction of cholesterol ester and triglyceride in human plasma very low density lipoprotein1 |
| topic | low density lipoprotein lipoprotein structure lipid phase behavior scanning calorimetry |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520416955 |
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