Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patients
IntroductionNeutrophil plays a more and more important role in sepsis with paralysis of immunoregulation. Till now, there was no biomarker to identify and isolate the mature and immature neutrophils in sepsis patients. CD10 shows on mature neutrophils at the latest stages of its differentiation. Our...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.1100756/full |
| _version_ | 1828071946185605120 |
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| author | Ming Liu Guan Wang Lin Wang Yuqi Wang Yuqing Bian Hang Shi Jie Liu |
| author_facet | Ming Liu Guan Wang Lin Wang Yuqi Wang Yuqing Bian Hang Shi Jie Liu |
| author_sort | Ming Liu |
| collection | DOAJ |
| description | IntroductionNeutrophil plays a more and more important role in sepsis with paralysis of immunoregulation. Till now, there was no biomarker to identify and isolate the mature and immature neutrophils in sepsis patients. CD10 shows on mature neutrophils at the latest stages of its differentiation. Our study aimed to investigate whether CD10 was a valid biomarker for distinguishing immature and mature neutrophil subgroups under septic conditions and their immunoregulatory effects on lymphocytes.MethodsTotally 80 healthy volunteers and 107 sepsis patients were recruited in this study. Fluorescence-conjugated anti-CD66b, and anti-CD10 monoclonal antibodies followed by incubation with specific anti-fluorochrome microbeads was used to isolate different subgroups of neutrophils. T cell apoptotic assays and T cell proliferation assays followed by flow cytometry analysis were used to evaluate the immunoregulatory effect of each subgroup of neutrophils.Results(1) The cytological morphology of CD10+ neutrophils was mature and that of CD10– neutrophils was immature in sepsis patients. (2) Mature CD10+ neutrophils inhibited the proliferation of T cell and immature CD10– neutrophils promoted the T cell proliferation.Conclusion(1) CD10 was a good biomarker to distinguish mature from immature neutrophils in sepsis patients. (2) Mature CD10+ and immature CD10– neutrophils displayed opposite immunoregulatory effects on T cells in sepsis patients. |
| first_indexed | 2024-04-11T01:01:34Z |
| format | Article |
| id | doaj.art-0609645c8efb42e2877d8d9e2492db53 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-11T01:01:34Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-0609645c8efb42e2877d8d9e2492db532023-01-04T15:44:24ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2023-01-01910.3389/fmed.2022.11007561100756Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patientsMing Liu0Guan Wang1Lin Wang2Yuqi Wang3Yuqing Bian4Hang Shi5Jie Liu6Department of Anesthesia, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Anesthesia, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Anesthesia, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Anesthesia, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Anesthesia, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Anesthesia, The Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaIntroductionNeutrophil plays a more and more important role in sepsis with paralysis of immunoregulation. Till now, there was no biomarker to identify and isolate the mature and immature neutrophils in sepsis patients. CD10 shows on mature neutrophils at the latest stages of its differentiation. Our study aimed to investigate whether CD10 was a valid biomarker for distinguishing immature and mature neutrophil subgroups under septic conditions and their immunoregulatory effects on lymphocytes.MethodsTotally 80 healthy volunteers and 107 sepsis patients were recruited in this study. Fluorescence-conjugated anti-CD66b, and anti-CD10 monoclonal antibodies followed by incubation with specific anti-fluorochrome microbeads was used to isolate different subgroups of neutrophils. T cell apoptotic assays and T cell proliferation assays followed by flow cytometry analysis were used to evaluate the immunoregulatory effect of each subgroup of neutrophils.Results(1) The cytological morphology of CD10+ neutrophils was mature and that of CD10– neutrophils was immature in sepsis patients. (2) Mature CD10+ neutrophils inhibited the proliferation of T cell and immature CD10– neutrophils promoted the T cell proliferation.Conclusion(1) CD10 was a good biomarker to distinguish mature from immature neutrophils in sepsis patients. (2) Mature CD10+ and immature CD10– neutrophils displayed opposite immunoregulatory effects on T cells in sepsis patients.https://www.frontiersin.org/articles/10.3389/fmed.2022.1100756/fullsepsisneutrophilCD10lymphocyteimmunoregulation |
| spellingShingle | Ming Liu Guan Wang Lin Wang Yuqi Wang Yuqing Bian Hang Shi Jie Liu Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patients Frontiers in Medicine sepsis neutrophil CD10 lymphocyte immunoregulation |
| title | Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patients |
| title_full | Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patients |
| title_fullStr | Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patients |
| title_full_unstemmed | Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patients |
| title_short | Immunoregulatory functions of mature CD10+ and immature CD10– neutrophils in sepsis patients |
| title_sort | immunoregulatory functions of mature cd10 and immature cd10 neutrophils in sepsis patients |
| topic | sepsis neutrophil CD10 lymphocyte immunoregulation |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.1100756/full |
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