<i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in Poland
Wheat (<i>Triticum aestivum</i>) is one of the most economically important crops in the world. During the routine monitoring of wheat pest, the cereal leaf beetle (CLB, <i>Oulema melanopus,</i> Coleoptera, Chrysomelidae), in the Greater Poland region, it was observed that som...
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MDPI AG
2020-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/12/1079 |
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| author | Krzysztof Krawczyk Beata Wielkopolan Aleksandra Obrępalska-Stęplowska |
| author_facet | Krzysztof Krawczyk Beata Wielkopolan Aleksandra Obrępalska-Stęplowska |
| author_sort | Krzysztof Krawczyk |
| collection | DOAJ |
| description | Wheat (<i>Triticum aestivum</i>) is one of the most economically important crops in the world. During the routine monitoring of wheat pest, the cereal leaf beetle (CLB, <i>Oulema melanopus,</i> Coleoptera, Chrysomelidae), in the Greater Poland region, it was observed that some leaves wounded by CLB also displayed brownish lesions with clear margins and yellow halo, disease symptoms resembling a bacterial infection. The aim of this study was therefore to investigate those symptoms to establish a causal agent of the disease. The identification based on the results of the Biolog’s Gen III system, 16S rRNA, and <i>gyr</i>B genes sequencing, revealed the presence of eight strains of <i>Pantoea ananatis</i> bacteria. Four strains were derived from wheat leaves (Ta024, Ta027, Ta030, Ta046), and four from the CLB’s oral secretion (OUC1, OUD2, OUF2, and OUG1). They shared the nucleotide identity ranging from 99 to 100% to <i>P. ananatis</i> strains deposited in the GenBank database. Additionally, the multi-locus sequence analysis (MLSA) of concatenated sequences of partial <i>atp</i>D, <i>fus</i>A, <i>gyr</i>B, <i>rpl</i>B, and <i>rpo</i>B genes was performed. All <i>P. ananatis</i> strains isolated in Poland, grouped into one cluster supported with high bootstrap value. Pathogenicity tests performed on four varieties of wheat plants have identified <i>P. ananatis</i> strains as a causal agent of wheat disease. To our knowledge, this is the first report of <i>P. ananatis</i> affecting wheat plants. |
| first_indexed | 2024-03-10T13:51:56Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T13:51:56Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-0609979de94b418fa2e4345e380f2f3f2023-11-21T01:59:22ZengMDPI AGPathogens2076-08172020-12-01912107910.3390/pathogens9121079<i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in PolandKrzysztof Krawczyk0Beata Wielkopolan1Aleksandra Obrępalska-Stęplowska2Department of Molecular Biology and Biotechnology, Institute of Plant Protection—National Research Institute, 20 Węgorka St., 60-318 Poznań, PolandDepartment of Monitoring and Signalling of Agrophages, Institute of Plant Protection—National Research Institute, 20 Węgorka St., 60-318 Poznań, PolandDepartment of Molecular Biology and Biotechnology, Institute of Plant Protection—National Research Institute, 20 Węgorka St., 60-318 Poznań, PolandWheat (<i>Triticum aestivum</i>) is one of the most economically important crops in the world. During the routine monitoring of wheat pest, the cereal leaf beetle (CLB, <i>Oulema melanopus,</i> Coleoptera, Chrysomelidae), in the Greater Poland region, it was observed that some leaves wounded by CLB also displayed brownish lesions with clear margins and yellow halo, disease symptoms resembling a bacterial infection. The aim of this study was therefore to investigate those symptoms to establish a causal agent of the disease. The identification based on the results of the Biolog’s Gen III system, 16S rRNA, and <i>gyr</i>B genes sequencing, revealed the presence of eight strains of <i>Pantoea ananatis</i> bacteria. Four strains were derived from wheat leaves (Ta024, Ta027, Ta030, Ta046), and four from the CLB’s oral secretion (OUC1, OUD2, OUF2, and OUG1). They shared the nucleotide identity ranging from 99 to 100% to <i>P. ananatis</i> strains deposited in the GenBank database. Additionally, the multi-locus sequence analysis (MLSA) of concatenated sequences of partial <i>atp</i>D, <i>fus</i>A, <i>gyr</i>B, <i>rpl</i>B, and <i>rpo</i>B genes was performed. All <i>P. ananatis</i> strains isolated in Poland, grouped into one cluster supported with high bootstrap value. Pathogenicity tests performed on four varieties of wheat plants have identified <i>P. ananatis</i> strains as a causal agent of wheat disease. To our knowledge, this is the first report of <i>P. ananatis</i> affecting wheat plants.https://www.mdpi.com/2076-0817/9/12/1079<i>Pantoea ananatis</i>wheatplant-pathogenic bacteriacereal leaf beetlewheat disease |
| spellingShingle | Krzysztof Krawczyk Beata Wielkopolan Aleksandra Obrępalska-Stęplowska <i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in Poland Pathogens <i>Pantoea ananatis</i> wheat plant-pathogenic bacteria cereal leaf beetle wheat disease |
| title | <i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in Poland |
| title_full | <i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in Poland |
| title_fullStr | <i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in Poland |
| title_full_unstemmed | <i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in Poland |
| title_short | <i>Pantoea ananatis</i>, A New Bacterial Pathogen Affecting Wheat Plants (<i>Triticum</i> L.) in Poland |
| title_sort | i pantoea ananatis i a new bacterial pathogen affecting wheat plants i triticum i l in poland |
| topic | <i>Pantoea ananatis</i> wheat plant-pathogenic bacteria cereal leaf beetle wheat disease |
| url | https://www.mdpi.com/2076-0817/9/12/1079 |
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