Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus
The mounting disquiets about the usage of precarious chemicals in the textile industry have steered to the development of eco-friendly and biological methods of fiber processing in the ever-escalating horizon of textile fibers. The current study targets the isolation, identification, and screening o...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2022-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-screening-and-identification-of-lignin-degraders-from-the-gut-of-termites-odontotermes-obesus/ |
| _version_ | 1798020843335319552 |
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| author | Revathy Rajan Ajith Sudhakaran Anita Ravindranath Rajathy Sivalingam Ratheesh Kumar |
| author_facet | Revathy Rajan Ajith Sudhakaran Anita Ravindranath Rajathy Sivalingam Ratheesh Kumar |
| author_sort | Revathy Rajan |
| collection | DOAJ |
| description | The mounting disquiets about the usage of precarious chemicals in the textile industry have steered to the development of eco-friendly and biological methods of fiber processing in the ever-escalating horizon of textile fibers. The current study targets the isolation, identification, and screening of lignin-degrading bacteria from termite gut microflora which could be employed in the textile trade, especially in coir industries for developing a biological method for softening coir fibers. Based on the morphology and taxonomic keys, termites used in the study were identified as Odontotermes obesus. The bacteria isolated from the termite gut having lignolytic activity were picked by using the methylene blue dye decolorizing method. The same was confirmed by using tannic acid. The isolates were then identified as Kosakonia oryzendophytica and Pseudomonas chengduensis by 16s rRNA sequencing. The isolates were further checked for their ability to produce extracellular lignolytic enzymes. The enzyme concentration was found to be significantly higher in the medium containing the microbial consortium than in those with the individual cultures. The consortium filtrate has MnP activity of 41.6 U/mL, LiP activity of 114.3 U/mL, and laccase activity of 61.85 U/mL at 72 hours of incubation. It was found that the enzyme activities were increased considerably until 72 hours of incubation but showed an insignificant increase at 96 hours. |
| first_indexed | 2024-04-11T17:04:20Z |
| format | Article |
| id | doaj.art-060b93bd1dec49a186d4a2205d3f63e3 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-11T17:04:20Z |
| publishDate | 2022-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-060b93bd1dec49a186d4a2205d3f63e32022-12-22T04:13:05ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-09-0116316961704https://doi.org/10.22207/JPAM.16.3.10Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesusRevathy Rajanhttps://orcid.org/0000-0001-8550-677XAjith Sudhakaranhttps://orcid.org/0000-0002-2541-2605Anita Ravindranathhttps://orcid.org/0000-0002-7631-9654Rajathy SivalingamRatheesh Kumarhttps://orcid.org/0000-0001-6358-9391The mounting disquiets about the usage of precarious chemicals in the textile industry have steered to the development of eco-friendly and biological methods of fiber processing in the ever-escalating horizon of textile fibers. The current study targets the isolation, identification, and screening of lignin-degrading bacteria from termite gut microflora which could be employed in the textile trade, especially in coir industries for developing a biological method for softening coir fibers. Based on the morphology and taxonomic keys, termites used in the study were identified as Odontotermes obesus. The bacteria isolated from the termite gut having lignolytic activity were picked by using the methylene blue dye decolorizing method. The same was confirmed by using tannic acid. The isolates were then identified as Kosakonia oryzendophytica and Pseudomonas chengduensis by 16s rRNA sequencing. The isolates were further checked for their ability to produce extracellular lignolytic enzymes. The enzyme concentration was found to be significantly higher in the medium containing the microbial consortium than in those with the individual cultures. The consortium filtrate has MnP activity of 41.6 U/mL, LiP activity of 114.3 U/mL, and laccase activity of 61.85 U/mL at 72 hours of incubation. It was found that the enzyme activities were increased considerably until 72 hours of incubation but showed an insignificant increase at 96 hours.https://microbiologyjournal.org/isolation-screening-and-identification-of-lignin-degraders-from-the-gut-of-termites-odontotermes-obesus/lignolyticperoxidaseslaccasefacultative anaerobe |
| spellingShingle | Revathy Rajan Ajith Sudhakaran Anita Ravindranath Rajathy Sivalingam Ratheesh Kumar Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus Journal of Pure and Applied Microbiology lignolytic peroxidases laccase facultative anaerobe |
| title | Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus |
| title_full | Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus |
| title_fullStr | Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus |
| title_full_unstemmed | Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus |
| title_short | Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus |
| title_sort | isolation screening and identification of lignin degraders from the gut of termites odontotermes obesus |
| topic | lignolytic peroxidases laccase facultative anaerobe |
| url | https://microbiologyjournal.org/isolation-screening-and-identification-of-lignin-degraders-from-the-gut-of-termites-odontotermes-obesus/ |
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