Phenotypic Changes in T and NK Cells Induced by Sputnik V Vaccination
A highly effective humoral immune response induced by the Sputnik V vaccine was demonstrated in independent studies, as well as in large-scale post-vaccination follow-up studies. However, the shifts in the cell-mediated immunity induced by Sputnik V vaccination are still under investigation. This st...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/11/6/1047 |
| _version_ | 1827735417507545088 |
|---|---|
| author | Anna A. Boyko Maria O. Ustiuzhanina Julia D. Vavilova Maria A. Streltsova Sofya A. Kust Andrei E. Siniavin Irina V. Astrakhantseva Marina S. Drutskaya Elena I. Kovalenko |
| author_facet | Anna A. Boyko Maria O. Ustiuzhanina Julia D. Vavilova Maria A. Streltsova Sofya A. Kust Andrei E. Siniavin Irina V. Astrakhantseva Marina S. Drutskaya Elena I. Kovalenko |
| author_sort | Anna A. Boyko |
| collection | DOAJ |
| description | A highly effective humoral immune response induced by the Sputnik V vaccine was demonstrated in independent studies, as well as in large-scale post-vaccination follow-up studies. However, the shifts in the cell-mediated immunity induced by Sputnik V vaccination are still under investigation. This study was aimed at estimating the impact of Sputnik V on activating and inhibitory receptors, activation and proliferative senescence markers in NK and T lymphocytes. The effects of Sputnik V were evaluated by the comparison of PBMC samples prior to vaccination, and then three days and three weeks following the second (boost) dose. The prime-boost format of Sputnik V vaccination induced a contraction in the T cell fraction of senescent CD57<sup>+</sup> cells and a decrease in HLA-DR-expressing T cells. The proportion of NKG2A<sup>+</sup> T cells was down-regulated after vaccination, whereas the PD-1 level was not affected significantly. A temporal increase in activation levels of NK cells and NKT-like cells was recorded, dependent on whether the individuals had COVID-19 prior to vaccination. A short-term elevation of the activating NKG2D and CD16 was observed in NK cells. Overall, the findings of the study are in favor of the Sputnik V vaccine not provoking a dramatic phenotypic rearrangement in T and NK cells, although it induces their slight temporal non-specific activation. |
| first_indexed | 2024-03-11T01:50:38Z |
| format | Article |
| id | doaj.art-060ee7c85ae148b6b32f2ac63c1ac71b |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T01:50:38Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-060ee7c85ae148b6b32f2ac63c1ac71b2023-11-18T12:58:28ZengMDPI AGVaccines2076-393X2023-05-01116104710.3390/vaccines11061047Phenotypic Changes in T and NK Cells Induced by Sputnik V VaccinationAnna A. Boyko0Maria O. Ustiuzhanina1Julia D. Vavilova2Maria A. Streltsova3Sofya A. Kust4Andrei E. Siniavin5Irina V. Astrakhantseva6Marina S. Drutskaya7Elena I. Kovalenko8Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaDivision of Immunobiology and Biomedicine, Center of Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Federal Territory Sirius, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaA highly effective humoral immune response induced by the Sputnik V vaccine was demonstrated in independent studies, as well as in large-scale post-vaccination follow-up studies. However, the shifts in the cell-mediated immunity induced by Sputnik V vaccination are still under investigation. This study was aimed at estimating the impact of Sputnik V on activating and inhibitory receptors, activation and proliferative senescence markers in NK and T lymphocytes. The effects of Sputnik V were evaluated by the comparison of PBMC samples prior to vaccination, and then three days and three weeks following the second (boost) dose. The prime-boost format of Sputnik V vaccination induced a contraction in the T cell fraction of senescent CD57<sup>+</sup> cells and a decrease in HLA-DR-expressing T cells. The proportion of NKG2A<sup>+</sup> T cells was down-regulated after vaccination, whereas the PD-1 level was not affected significantly. A temporal increase in activation levels of NK cells and NKT-like cells was recorded, dependent on whether the individuals had COVID-19 prior to vaccination. A short-term elevation of the activating NKG2D and CD16 was observed in NK cells. Overall, the findings of the study are in favor of the Sputnik V vaccine not provoking a dramatic phenotypic rearrangement in T and NK cells, although it induces their slight temporal non-specific activation.https://www.mdpi.com/2076-393X/11/6/1047Sputnik VT lymphocytesNK cellsactivating and inhibitory receptorsactivation markersproliferative senescence |
| spellingShingle | Anna A. Boyko Maria O. Ustiuzhanina Julia D. Vavilova Maria A. Streltsova Sofya A. Kust Andrei E. Siniavin Irina V. Astrakhantseva Marina S. Drutskaya Elena I. Kovalenko Phenotypic Changes in T and NK Cells Induced by Sputnik V Vaccination Vaccines Sputnik V T lymphocytes NK cells activating and inhibitory receptors activation markers proliferative senescence |
| title | Phenotypic Changes in T and NK Cells Induced by Sputnik V Vaccination |
| title_full | Phenotypic Changes in T and NK Cells Induced by Sputnik V Vaccination |
| title_fullStr | Phenotypic Changes in T and NK Cells Induced by Sputnik V Vaccination |
| title_full_unstemmed | Phenotypic Changes in T and NK Cells Induced by Sputnik V Vaccination |
| title_short | Phenotypic Changes in T and NK Cells Induced by Sputnik V Vaccination |
| title_sort | phenotypic changes in t and nk cells induced by sputnik v vaccination |
| topic | Sputnik V T lymphocytes NK cells activating and inhibitory receptors activation markers proliferative senescence |
| url | https://www.mdpi.com/2076-393X/11/6/1047 |
| work_keys_str_mv | AT annaaboyko phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT mariaoustiuzhanina phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT juliadvavilova phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT mariaastreltsova phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT sofyaakust phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT andreiesiniavin phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT irinavastrakhantseva phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT marinasdrutskaya phenotypicchangesintandnkcellsinducedbysputnikvvaccination AT elenaikovalenko phenotypicchangesintandnkcellsinducedbysputnikvvaccination |