Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks
Summary The possibilities of human pluripotent stem cell-derived neural cells from the basic research tool to a treatment option in regenerative medicine have been well recognized. These cells also offer an interesting tool for in vitro models of neuronal networks to be used for drug screening and n...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2013-05-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/2/6/605 |
| _version_ | 1818667706738540544 |
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| author | Heikki Kiiski Riikka Äänismaa Jyrki Tenhunen Sanna Hagman Laura Ylä-Outinen Antti Aho Arvi Yli-Hankala Stepani Bendel Heli Skottman Susanna Narkilahti |
| author_facet | Heikki Kiiski Riikka Äänismaa Jyrki Tenhunen Sanna Hagman Laura Ylä-Outinen Antti Aho Arvi Yli-Hankala Stepani Bendel Heli Skottman Susanna Narkilahti |
| author_sort | Heikki Kiiski |
| collection | DOAJ |
| description | Summary
The possibilities of human pluripotent stem cell-derived neural cells from the basic research tool to a treatment option in regenerative medicine have been well recognized. These cells also offer an interesting tool for in vitro models of neuronal networks to be used for drug screening and neurotoxicological studies and for patient/disease specific in vitro models. Here, as aiming to develop a reductionistic in vitro human neuronal network model, we tested whether human embryonic stem cell (hESC)-derived neural cells could be cultured in human cerebrospinal fluid (CSF) in order to better mimic the in vivo conditions. Our results showed that CSF altered the differentiation of hESC-derived neural cells towards glial cells at the expense of neuronal differentiation. The proliferation rate was reduced in CSF cultures. However, even though the use of CSF as the culture medium altered the glial vs. neuronal differentiation rate, the pre-existing spontaneous activity of the neuronal networks persisted throughout the study. These results suggest that it is possible to develop fully human cell and culture-based environments that can further be modified for various in vitro modeling purposes. |
| first_indexed | 2024-12-17T06:24:41Z |
| format | Article |
| id | doaj.art-06137749ea2d4ae3aa9acabda83b8d8a |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T06:24:41Z |
| publishDate | 2013-05-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-06137749ea2d4ae3aa9acabda83b8d8a2022-12-21T22:00:19ZengThe Company of BiologistsBiology Open2046-63902013-05-012660561210.1242/bio.2013464820134648Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networksHeikki Kiiski0Riikka Äänismaa1Jyrki Tenhunen2Sanna Hagman3Laura Ylä-Outinen4Antti Aho5Arvi Yli-Hankala6Stepani Bendel7Heli Skottman8Susanna Narkilahti9 Critical Care Medicine Research Group, Department of Intensive Care Unit, Tampere University Hospital, FI-33521 Tampere, Finland NeuroGroup, Institute of Biomedical Technology/BioMediTech, University of Tampere, FI-33520 Tampere, Finland Critical Care Medicine Research Group, Department of Intensive Care Unit, Tampere University Hospital, FI-33521 Tampere, Finland Neuroimmunology Unit, Medical School, University of Tampere and Tampere University Hospital, FI-33014 Tampere, Finland NeuroGroup, Institute of Biomedical Technology/BioMediTech, University of Tampere, FI-33520 Tampere, Finland Coxa – Hospital for Joint Replacement, FI-33520 Tampere, Finland Department of Anesthesia, Tampere University Hospital, FI-33521 Tampere, Finland Department of Intensive Care Medicine, Kuopio University Hospital, FI-70029 Kuopio, Finland Opthalmology Group, Institute of Biomedical Technology/BioMediTech, University of Tampere, FI-33520 Tampere, Finland NeuroGroup, Institute of Biomedical Technology/BioMediTech, University of Tampere, FI-33520 Tampere, Finland Summary The possibilities of human pluripotent stem cell-derived neural cells from the basic research tool to a treatment option in regenerative medicine have been well recognized. These cells also offer an interesting tool for in vitro models of neuronal networks to be used for drug screening and neurotoxicological studies and for patient/disease specific in vitro models. Here, as aiming to develop a reductionistic in vitro human neuronal network model, we tested whether human embryonic stem cell (hESC)-derived neural cells could be cultured in human cerebrospinal fluid (CSF) in order to better mimic the in vivo conditions. Our results showed that CSF altered the differentiation of hESC-derived neural cells towards glial cells at the expense of neuronal differentiation. The proliferation rate was reduced in CSF cultures. However, even though the use of CSF as the culture medium altered the glial vs. neuronal differentiation rate, the pre-existing spontaneous activity of the neuronal networks persisted throughout the study. These results suggest that it is possible to develop fully human cell and culture-based environments that can further be modified for various in vitro modeling purposes.http://bio.biologists.org/content/2/6/605AstrocyteMicroelectrode arrayNetwork activityNeuronOligodendrocyteStem cell |
| spellingShingle | Heikki Kiiski Riikka Äänismaa Jyrki Tenhunen Sanna Hagman Laura Ylä-Outinen Antti Aho Arvi Yli-Hankala Stepani Bendel Heli Skottman Susanna Narkilahti Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks Biology Open Astrocyte Microelectrode array Network activity Neuron Oligodendrocyte Stem cell |
| title | Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks |
| title_full | Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks |
| title_fullStr | Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks |
| title_full_unstemmed | Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks |
| title_short | Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks |
| title_sort | healthy human csf promotes glial differentiation of hesc derived neural cells while retaining spontaneous activity in existing neuronal networks |
| topic | Astrocyte Microelectrode array Network activity Neuron Oligodendrocyte Stem cell |
| url | http://bio.biologists.org/content/2/6/605 |
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