Cryopreservation of the Microalgae <i>Scenedesmus</i> sp.
Each phytoplankton species presents a different behavior and tolerance to the cryopreservation process. Therefore, in a species-specific protocol, it is essential to ensure both growth and post-thawing cell viability. In this study, we explored the effect of cryopreservation of <i>Scenedesmus&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/4/562 |
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| author | Martha Prieto-Guevara Jany Alarcón-Furnieles César Jiménez-Velásquez Yamid Hernández-Julio José Espinosa-Araujo Víctor Atencio-García |
| author_facet | Martha Prieto-Guevara Jany Alarcón-Furnieles César Jiménez-Velásquez Yamid Hernández-Julio José Espinosa-Araujo Víctor Atencio-García |
| author_sort | Martha Prieto-Guevara |
| collection | DOAJ |
| description | Each phytoplankton species presents a different behavior and tolerance to the cryopreservation process. Therefore, in a species-specific protocol, it is essential to ensure both growth and post-thawing cell viability. In this study, we explored the effect of cryopreservation of <i>Scenedesmus</i> sp. with two cryoprotectants, dimethyl sulfoxide (DMSO) and methanol (MET), at 5% and 10% inclusion for each. In the control treatment, the microalgae were not exposed to cryoprotective agents (Control). Three post-thawing cell viability criteria were used: no cell damage (NCD), cell damage (CD), and marked lesions (LM), and mitochondrial and cell membrane damage was evaluated by flow cytometry. The study was a 2 × 2 factorial design, with five replications by treatments, population growth, and cell damage evaluated from the fifth day after thawing. On the fifth day, the highest percentage of NCD was observed when the microalgae were cryopreserved with DMSO 5% (50%); Regarding the control group, it showed 0% NCD. Flow cytometry analysis reveals minor damage at the membrane and mitochondria (9–10.7%) when DMSO is used at both inclusion percentages (5–10%) after thawing. In the exponential phase, the highest growth rates, doubling time, and yield was observed in cryopreserved cells with MET 5%. The results suggest that DMSO 5% is an ideal treatment for cryopreserving microalgae <i>Scenedesmus</i> sp. |
| first_indexed | 2024-03-11T09:00:32Z |
| format | Article |
| id | doaj.art-061a90f161934a19aa3157a1ca9e20a9 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T09:00:32Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-061a90f161934a19aa3157a1ca9e20a92023-11-16T19:44:04ZengMDPI AGCells2073-44092023-02-0112456210.3390/cells12040562Cryopreservation of the Microalgae <i>Scenedesmus</i> sp.Martha Prieto-Guevara0Jany Alarcón-Furnieles1César Jiménez-Velásquez2Yamid Hernández-Julio3José Espinosa-Araujo4Víctor Atencio-García5CINPIC, Fish Culture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Cra 6, 77-305, Montería 230002, ColombiaSchool of Basic Sciences, Department of Biology, University of Córdoba, Cra 6, Montería 77-305, ColombiaCINPIC, Fish Culture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Cra 6, 77-305, Montería 230002, ColombiaFaculty of Economics, Management and Accounting Sciences, Universidad del Sinú Elías Bechara Zainúm, Montería 230002, ColombiaCINPIC, Fish Culture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Cra 6, 77-305, Montería 230002, ColombiaCINPIC, Fish Culture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Cra 6, 77-305, Montería 230002, ColombiaEach phytoplankton species presents a different behavior and tolerance to the cryopreservation process. Therefore, in a species-specific protocol, it is essential to ensure both growth and post-thawing cell viability. In this study, we explored the effect of cryopreservation of <i>Scenedesmus</i> sp. with two cryoprotectants, dimethyl sulfoxide (DMSO) and methanol (MET), at 5% and 10% inclusion for each. In the control treatment, the microalgae were not exposed to cryoprotective agents (Control). Three post-thawing cell viability criteria were used: no cell damage (NCD), cell damage (CD), and marked lesions (LM), and mitochondrial and cell membrane damage was evaluated by flow cytometry. The study was a 2 × 2 factorial design, with five replications by treatments, population growth, and cell damage evaluated from the fifth day after thawing. On the fifth day, the highest percentage of NCD was observed when the microalgae were cryopreserved with DMSO 5% (50%); Regarding the control group, it showed 0% NCD. Flow cytometry analysis reveals minor damage at the membrane and mitochondria (9–10.7%) when DMSO is used at both inclusion percentages (5–10%) after thawing. In the exponential phase, the highest growth rates, doubling time, and yield was observed in cryopreserved cells with MET 5%. The results suggest that DMSO 5% is an ideal treatment for cryopreserving microalgae <i>Scenedesmus</i> sp.https://www.mdpi.com/2073-4409/12/4/562aquaculturechlorophyceaecryoprotectants |
| spellingShingle | Martha Prieto-Guevara Jany Alarcón-Furnieles César Jiménez-Velásquez Yamid Hernández-Julio José Espinosa-Araujo Víctor Atencio-García Cryopreservation of the Microalgae <i>Scenedesmus</i> sp. Cells aquaculture chlorophyceae cryoprotectants |
| title | Cryopreservation of the Microalgae <i>Scenedesmus</i> sp. |
| title_full | Cryopreservation of the Microalgae <i>Scenedesmus</i> sp. |
| title_fullStr | Cryopreservation of the Microalgae <i>Scenedesmus</i> sp. |
| title_full_unstemmed | Cryopreservation of the Microalgae <i>Scenedesmus</i> sp. |
| title_short | Cryopreservation of the Microalgae <i>Scenedesmus</i> sp. |
| title_sort | cryopreservation of the microalgae i scenedesmus i sp |
| topic | aquaculture chlorophyceae cryoprotectants |
| url | https://www.mdpi.com/2073-4409/12/4/562 |
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