Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo Studies
The use of phytogenic extracts is considered a sustainable strategy for the prevention of fish diseases, including <i>Alliaceae</i> as a potential option due to their variety of bioactive compounds. In this study, we analyzed the antibacterial and antiparasitic potential of propyl-propan...
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MDPI AG
2022-10-01
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| author | Jose F. Cabello-Gómez María Arántzazu Aguinaga-Casañas Ana Falcón-Piñeiro Elías González-Gragera Raquel Márquez-Martín María del Mar Agraso Laura Bermúdez Alberto Baños Manuel Martínez-Bueno |
| author_facet | Jose F. Cabello-Gómez María Arántzazu Aguinaga-Casañas Ana Falcón-Piñeiro Elías González-Gragera Raquel Márquez-Martín María del Mar Agraso Laura Bermúdez Alberto Baños Manuel Martínez-Bueno |
| author_sort | Jose F. Cabello-Gómez |
| collection | DOAJ |
| description | The use of phytogenic extracts is considered a sustainable strategy for the prevention of fish diseases, including <i>Alliaceae</i> as a potential option due to their variety of bioactive compounds. In this study, we analyzed the antibacterial and antiparasitic potential of propyl-propane-thiosulfinate (PTS) and propyl-propane-thiosulfonate (PTSO) from onions. The in vitro activity against <i>Pseudomonas anguilliseptica</i>, <i>Tenacibaculum maritimum</i>, and <i>Photobacterium damselae</i> of both compounds was tested. In addition, the viability of <i>Sparicotyle chrysophrii</i> larvae was evaluated. Moreover, a diet that consisted of a blend of PTS/PTSO (ALLIUM) was used. A total of 90 gilthead sea bream juveniles were tested against <i>P. damselae</i> subsp. <i>Piscicida</i> after 12 weeks of dietary administration. Furthermore, 150 fish with a rate of 10–15 parasites/fish were fed for 21 days and the number of gill parasites was recorded. All strains were sensitive to both compounds. PTSO showed the highest inhibitory effect against all target strains, while PTS showed higher effectiveness against <i>S. chrysophrii.</i> Fish from ALLIUM group presented the highest probability of survival, increasing up to 91.1%, whereas in the control group, the probability of survival was 66.7%. The number of parasites in the gilthead sea bream decreased in the ALLIUM group over time. These results suggest the inclusion of PTS and PTSO in feed as a natural strategy to prevent antibacterial and antiparasitic fish diseases. |
| first_indexed | 2024-03-09T19:42:01Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:42:01Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-061b5164660b423ea3d6c1c8e286a41a2023-11-24T01:33:23ZengMDPI AGMolecules1420-30492022-10-012720690010.3390/molecules27206900Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo StudiesJose F. Cabello-Gómez0María Arántzazu Aguinaga-Casañas1Ana Falcón-Piñeiro2Elías González-Gragera3Raquel Márquez-Martín4María del Mar Agraso5Laura Bermúdez6Alberto Baños7Manuel Martínez-Bueno8Aquaculture Technology Centre of Andalusia, CTAQUA, Muelle Comercial s/n, El Puerto de Santa María, 11500 Cadiz, SpainDepartment of Microbiology and Biotechnology, DMC Research Center, Camino de Jayena s/n, 18620 Granada, SpainDepartment of Microbiology and Biotechnology, DMC Research Center, Camino de Jayena s/n, 18620 Granada, SpainDepartment of Microbiology and Biotechnology, DMC Research Center, Camino de Jayena s/n, 18620 Granada, SpainDepartment of Microbiology and Biotechnology, DMC Research Center, Camino de Jayena s/n, 18620 Granada, SpainAquaculture Technology Centre of Andalusia, CTAQUA, Muelle Comercial s/n, El Puerto de Santa María, 11500 Cadiz, SpainAquaculture Technology Centre of Andalusia, CTAQUA, Muelle Comercial s/n, El Puerto de Santa María, 11500 Cadiz, SpainDepartment of Microbiology and Biotechnology, DMC Research Center, Camino de Jayena s/n, 18620 Granada, SpainDepartment of Microbiology, University of Granada, Fuente Nueva s/n, 18071 Granada, SpainThe use of phytogenic extracts is considered a sustainable strategy for the prevention of fish diseases, including <i>Alliaceae</i> as a potential option due to their variety of bioactive compounds. In this study, we analyzed the antibacterial and antiparasitic potential of propyl-propane-thiosulfinate (PTS) and propyl-propane-thiosulfonate (PTSO) from onions. The in vitro activity against <i>Pseudomonas anguilliseptica</i>, <i>Tenacibaculum maritimum</i>, and <i>Photobacterium damselae</i> of both compounds was tested. In addition, the viability of <i>Sparicotyle chrysophrii</i> larvae was evaluated. Moreover, a diet that consisted of a blend of PTS/PTSO (ALLIUM) was used. A total of 90 gilthead sea bream juveniles were tested against <i>P. damselae</i> subsp. <i>Piscicida</i> after 12 weeks of dietary administration. Furthermore, 150 fish with a rate of 10–15 parasites/fish were fed for 21 days and the number of gill parasites was recorded. All strains were sensitive to both compounds. PTSO showed the highest inhibitory effect against all target strains, while PTS showed higher effectiveness against <i>S. chrysophrii.</i> Fish from ALLIUM group presented the highest probability of survival, increasing up to 91.1%, whereas in the control group, the probability of survival was 66.7%. The number of parasites in the gilthead sea bream decreased in the ALLIUM group over time. These results suggest the inclusion of PTS and PTSO in feed as a natural strategy to prevent antibacterial and antiparasitic fish diseases.https://www.mdpi.com/1420-3049/27/20/6900<i>Photobacterium damselae</i><i>Sparicotyle chrysophrii</i>antibacterialantiparasitic<i>Allium</i>phytogenics |
| spellingShingle | Jose F. Cabello-Gómez María Arántzazu Aguinaga-Casañas Ana Falcón-Piñeiro Elías González-Gragera Raquel Márquez-Martín María del Mar Agraso Laura Bermúdez Alberto Baños Manuel Martínez-Bueno Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo Studies Molecules <i>Photobacterium damselae</i> <i>Sparicotyle chrysophrii</i> antibacterial antiparasitic <i>Allium</i> phytogenics |
| title | Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo Studies |
| title_full | Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo Studies |
| title_fullStr | Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo Studies |
| title_full_unstemmed | Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo Studies |
| title_short | Antibacterial and Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from <i>Allium cepa</i> against Gilthead Sea Bream Pathogens in In Vitro and In Vivo Studies |
| title_sort | antibacterial and antiparasitic activity of propyl propane thiosulfinate pts and propyl propane thiosulfonate ptso from i allium cepa i against gilthead sea bream pathogens in in vitro and in vivo studies |
| topic | <i>Photobacterium damselae</i> <i>Sparicotyle chrysophrii</i> antibacterial antiparasitic <i>Allium</i> phytogenics |
| url | https://www.mdpi.com/1420-3049/27/20/6900 |
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