Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269
Indolocarbazole glycosidic derivative LCS-1269 with significant antiproliferative activity has been synthesized in N.N. Blokhin National Medical Research Center of Oncology. To control the quality of the substance, the chromatographic method of the assay was created and validated. The technique was...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2021-05-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/63457/download/pdf/ |
| _version_ | 1818350228885995520 |
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| author | Zoya Shprakh Andrei Budko Dmitry Kozin Lydia Ektova Dmitry Gusev Vladimir Reshetnyak Olga Nesterova |
| author_facet | Zoya Shprakh Andrei Budko Dmitry Kozin Lydia Ektova Dmitry Gusev Vladimir Reshetnyak Olga Nesterova |
| author_sort | Zoya Shprakh |
| collection | DOAJ |
| description | Indolocarbazole glycosidic derivative LCS-1269 with significant antiproliferative activity has been synthesized in N.N. Blokhin National Medical Research Center of Oncology. To control the quality of the substance, the chromatographic method of the assay was created and validated. The technique was carried out in a gradient mode using mobile phases consist of acetonitrile, trifluoroacetic acid and purified water. The specificity of the method was shown by checking of test solutions and the special solvent chromatograms. The method linearity was confirmed, and the parameters of linear dependence have been estimated, and the relationship was described by the equation: y = 49.23× – 35.51 with correlation coefficient 0.9998. The method’s precision was determined as the repeatability with a relative error of the mean 1.49% and was 2.433 ± 0.036. Was shown, that the results obtained in the intermediate precision estimation were not burdened with a systematic error. The detection limit and quantitation limit were calculated based on the linear relationship data as 3.15 μg/mL and 9.57 μg/mL, respectively. Sensitive HPLC method for LCS-1269 assay in substance has been developed and validated. |
| first_indexed | 2024-12-13T18:18:31Z |
| format | Article |
| id | doaj.art-061ee92c444b47e0b79a1b7f90c9482c |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-12-13T18:18:31Z |
| publishDate | 2021-05-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-061ee92c444b47e0b79a1b7f90c9482c2022-12-21T23:35:47ZengPensoft PublishersPharmacia2603-557X2021-05-0168242743110.3897/pharmacia.68.e6345763457Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269Zoya Shprakh0Andrei Budko1Dmitry Kozin2Lydia Ektova3Dmitry Gusev4Vladimir Reshetnyak5Olga Nesterova6I.M. Sechenov First Moscow State Medical UniversityN.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationI.M. Sechenov First Moscow State Medical UniversityI.M. Sechenov First Moscow State Medical UniversityIndolocarbazole glycosidic derivative LCS-1269 with significant antiproliferative activity has been synthesized in N.N. Blokhin National Medical Research Center of Oncology. To control the quality of the substance, the chromatographic method of the assay was created and validated. The technique was carried out in a gradient mode using mobile phases consist of acetonitrile, trifluoroacetic acid and purified water. The specificity of the method was shown by checking of test solutions and the special solvent chromatograms. The method linearity was confirmed, and the parameters of linear dependence have been estimated, and the relationship was described by the equation: y = 49.23× – 35.51 with correlation coefficient 0.9998. The method’s precision was determined as the repeatability with a relative error of the mean 1.49% and was 2.433 ± 0.036. Was shown, that the results obtained in the intermediate precision estimation were not burdened with a systematic error. The detection limit and quantitation limit were calculated based on the linear relationship data as 3.15 μg/mL and 9.57 μg/mL, respectively. Sensitive HPLC method for LCS-1269 assay in substance has been developed and validated.https://pharmacia.pensoft.net/article/63457/download/pdf/ |
| spellingShingle | Zoya Shprakh Andrei Budko Dmitry Kozin Lydia Ektova Dmitry Gusev Vladimir Reshetnyak Olga Nesterova Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269 Pharmacia |
| title | Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269 |
| title_full | Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269 |
| title_fullStr | Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269 |
| title_full_unstemmed | Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269 |
| title_short | Development and validation of HPLC method for analysis of indolocarbazole derivative LCS-1269 |
| title_sort | development and validation of hplc method for analysis of indolocarbazole derivative lcs 1269 |
| url | https://pharmacia.pensoft.net/article/63457/download/pdf/ |
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