Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoea
ABSTRACT: Objectives: Antibiotics are associated with increased risk of Clostridioides difficile infection, which has limited treatment options. We assessed in vitro activity of omadacycline (an aminomethylcycline antibiotic) against the C. difficile infection strain and efficacy in a hamster model...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522000960 |
| _version_ | 1798003240450654208 |
|---|---|
| author | Raina Gay Leahy Alisa W. Serio Kelly Wright Maria M. Traczewski S. Ken Tanaka |
| author_facet | Raina Gay Leahy Alisa W. Serio Kelly Wright Maria M. Traczewski S. Ken Tanaka |
| author_sort | Raina Gay Leahy |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Antibiotics are associated with increased risk of Clostridioides difficile infection, which has limited treatment options. We assessed in vitro activity of omadacycline (an aminomethylcycline antibiotic) against the C. difficile infection strain and efficacy in a hamster model of C. difficile-associated diarrhoea. Methods: Omadacycline, clindamycin, tigecycline, vancomycin, and metronidazole minimum inhibitory concentrations (MICs) for the infection-model strain (C. difficile ATCC 43596) were determined. Hamsters were pretreated with subcutaneous clindamycin (10 mg/kg) and infected 24 h later with C. difficile ATCC 43596; 24 h post infection, they received oral omadacycline (50 mg/kg/day), vancomycin (50 mg/kg/day), or vehicle for 5 days. Efficacy was reported as survival. Results: Omadacycline was as active as tigecycline, vancomycin, and metronidazole (MIC 0.06 mg/L); clindamycin showed no activity. Median survival in hamsters was: 12 days, omadacycline; 2 days, vancomycin; 4 days, clindamycin pretreatment only. Conclusion: Omadacycline exhibited potent in vitro activity against C. difficile and showed efficacy in a model of C. difficile-associated diarrhoea. |
| first_indexed | 2024-04-11T12:05:50Z |
| format | Article |
| id | doaj.art-061fc20000c14acfb1c1d713fad6c482 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-11T12:05:50Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-061fc20000c14acfb1c1d713fad6c4822022-12-22T04:24:44ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-09-01309699Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoeaRaina Gay Leahy0Alisa W. Serio1Kelly Wright2Maria M. Traczewski3S. Ken Tanaka4Paratek Pharmaceuticals, Inc., King of Prussia, Pennsylvania, USAParatek Pharmaceuticals, Inc., King of Prussia, Pennsylvania, USA; Corresponding author. 1000 First Avenue, Suite 200, King of Prussia, PA 19406, USA.Paratek Pharmaceuticals, Inc., King of Prussia, Pennsylvania, USAClinical Microbiology Institute, Inc., Wilsonville, Oregon, USAParatek Pharmaceuticals, Inc., King of Prussia, Pennsylvania, USAABSTRACT: Objectives: Antibiotics are associated with increased risk of Clostridioides difficile infection, which has limited treatment options. We assessed in vitro activity of omadacycline (an aminomethylcycline antibiotic) against the C. difficile infection strain and efficacy in a hamster model of C. difficile-associated diarrhoea. Methods: Omadacycline, clindamycin, tigecycline, vancomycin, and metronidazole minimum inhibitory concentrations (MICs) for the infection-model strain (C. difficile ATCC 43596) were determined. Hamsters were pretreated with subcutaneous clindamycin (10 mg/kg) and infected 24 h later with C. difficile ATCC 43596; 24 h post infection, they received oral omadacycline (50 mg/kg/day), vancomycin (50 mg/kg/day), or vehicle for 5 days. Efficacy was reported as survival. Results: Omadacycline was as active as tigecycline, vancomycin, and metronidazole (MIC 0.06 mg/L); clindamycin showed no activity. Median survival in hamsters was: 12 days, omadacycline; 2 days, vancomycin; 4 days, clindamycin pretreatment only. Conclusion: Omadacycline exhibited potent in vitro activity against C. difficile and showed efficacy in a model of C. difficile-associated diarrhoea.http://www.sciencedirect.com/science/article/pii/S2213716522000960Clostridioides difficileClostridium difficileOmadacyclineHamster modelSurvivalMinimum inhibitory concentration |
| spellingShingle | Raina Gay Leahy Alisa W. Serio Kelly Wright Maria M. Traczewski S. Ken Tanaka Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoea Journal of Global Antimicrobial Resistance Clostridioides difficile Clostridium difficile Omadacycline Hamster model Survival Minimum inhibitory concentration |
| title | Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoea |
| title_full | Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoea |
| title_fullStr | Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoea |
| title_full_unstemmed | Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoea |
| title_short | Activity of omadacycline in vitro against Clostridioides difficile and preliminary efficacy assessment in a hamster model of C. difficile-associated diarrhoea |
| title_sort | activity of omadacycline in vitro against clostridioides difficile and preliminary efficacy assessment in a hamster model of c difficile associated diarrhoea |
| topic | Clostridioides difficile Clostridium difficile Omadacycline Hamster model Survival Minimum inhibitory concentration |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522000960 |
| work_keys_str_mv | AT rainagayleahy activityofomadacyclineinvitroagainstclostridioidesdifficileandpreliminaryefficacyassessmentinahamstermodelofcdifficileassociateddiarrhoea AT alisawserio activityofomadacyclineinvitroagainstclostridioidesdifficileandpreliminaryefficacyassessmentinahamstermodelofcdifficileassociateddiarrhoea AT kellywright activityofomadacyclineinvitroagainstclostridioidesdifficileandpreliminaryefficacyassessmentinahamstermodelofcdifficileassociateddiarrhoea AT mariamtraczewski activityofomadacyclineinvitroagainstclostridioidesdifficileandpreliminaryefficacyassessmentinahamstermodelofcdifficileassociateddiarrhoea AT skentanaka activityofomadacyclineinvitroagainstclostridioidesdifficileandpreliminaryefficacyassessmentinahamstermodelofcdifficileassociateddiarrhoea |