Effect of ghrelin on inflammatory response in lung contusion
The purpose of this study was to investigate the effects of ghrelin on inflammatory response and tissue damage following trauma-induced acute lung injury. Thirty male wistar albino rats (300–400 g) were randomly assigned into three groups: control group (n = 6), lung contusion plus saline (saline-tr...
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| Format: | Article |
| Language: | English |
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Wiley
2013-02-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X12002124 |
| _version_ | 1811276676436328448 |
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| author | Berrak Guven Mertol Gokce Ozkan Saydam Murat Can Sibel Bektas Serhan Yurtlu |
| author_facet | Berrak Guven Mertol Gokce Ozkan Saydam Murat Can Sibel Bektas Serhan Yurtlu |
| author_sort | Berrak Guven |
| collection | DOAJ |
| description | The purpose of this study was to investigate the effects of ghrelin on inflammatory response and tissue damage following trauma-induced acute lung injury. Thirty male wistar albino rats (300–400 g) were randomly assigned into three groups: control group (n = 6), lung contusion plus saline (saline-treated, n = 12), and lung contusion plus ghrelin (ghrelin-treated, n = 12). Saline- or ghrelin-treated traumatic rats were sacrificed at two time points (24 and 72 hours) after lung contusion. Blood was collected for the analysis of serum adenosine deaminase (ADA). Tissue transforming growth factor-beta 1 (TGF-β1) and matrix metalloproteinase-2 (MMP-2) levels were measured by enzyme-linked immunosorbent assay and histopathological examination was performed on the lung tissue samples. Our results indicated that ghrelin significantly reduced morphologic damages. Serum ADA activities were significantly decreased after lung contusion and this decline started early with ghrelin treatment. TGF-β1 and MMP-2 levels in lung tissue were elevated at 72 hours after lung contusion and treatment with ghrelin significantly increased TGF-β1 level and reduced MMP-2 level. In conclusion, our study demonstrates that acute lung injury initiated proinflammatory responses and ghrelin administration showed an anti-inflammatory effect in lung contusion. |
| first_indexed | 2024-04-13T00:02:02Z |
| format | Article |
| id | doaj.art-061fe667aebb4e54a9269e5a528ac614 |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-04-13T00:02:02Z |
| publishDate | 2013-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-061fe667aebb4e54a9269e5a528ac6142022-12-22T03:11:19ZengWileyKaohsiung Journal of Medical Sciences1607-551X2013-02-01292697410.1016/j.kjms.2012.08.011Effect of ghrelin on inflammatory response in lung contusionBerrak Guven0Mertol Gokce1Ozkan Saydam2Murat Can3Sibel Bektas4Serhan Yurtlu5Department of Biochemistry, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, TurkeyDepartment of Thoracic Surgery, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, TurkeyDepartment of Thoracic Surgery, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, TurkeyDepartment of Biochemistry, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, TurkeyDepartment of Pathology, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, TurkeyDepartment of Anesthesiology, Faculty of Medicine, Bulent Ecevit University (Formerly Karaelmas University), Zonguldak, TurkeyThe purpose of this study was to investigate the effects of ghrelin on inflammatory response and tissue damage following trauma-induced acute lung injury. Thirty male wistar albino rats (300–400 g) were randomly assigned into three groups: control group (n = 6), lung contusion plus saline (saline-treated, n = 12), and lung contusion plus ghrelin (ghrelin-treated, n = 12). Saline- or ghrelin-treated traumatic rats were sacrificed at two time points (24 and 72 hours) after lung contusion. Blood was collected for the analysis of serum adenosine deaminase (ADA). Tissue transforming growth factor-beta 1 (TGF-β1) and matrix metalloproteinase-2 (MMP-2) levels were measured by enzyme-linked immunosorbent assay and histopathological examination was performed on the lung tissue samples. Our results indicated that ghrelin significantly reduced morphologic damages. Serum ADA activities were significantly decreased after lung contusion and this decline started early with ghrelin treatment. TGF-β1 and MMP-2 levels in lung tissue were elevated at 72 hours after lung contusion and treatment with ghrelin significantly increased TGF-β1 level and reduced MMP-2 level. In conclusion, our study demonstrates that acute lung injury initiated proinflammatory responses and ghrelin administration showed an anti-inflammatory effect in lung contusion.http://www.sciencedirect.com/science/article/pii/S1607551X12002124Adenosine deaminaseGhrelinLung contusionMMP-2TGF-β1 |
| spellingShingle | Berrak Guven Mertol Gokce Ozkan Saydam Murat Can Sibel Bektas Serhan Yurtlu Effect of ghrelin on inflammatory response in lung contusion Kaohsiung Journal of Medical Sciences Adenosine deaminase Ghrelin Lung contusion MMP-2 TGF-β1 |
| title | Effect of ghrelin on inflammatory response in lung contusion |
| title_full | Effect of ghrelin on inflammatory response in lung contusion |
| title_fullStr | Effect of ghrelin on inflammatory response in lung contusion |
| title_full_unstemmed | Effect of ghrelin on inflammatory response in lung contusion |
| title_short | Effect of ghrelin on inflammatory response in lung contusion |
| title_sort | effect of ghrelin on inflammatory response in lung contusion |
| topic | Adenosine deaminase Ghrelin Lung contusion MMP-2 TGF-β1 |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X12002124 |
| work_keys_str_mv | AT berrakguven effectofghrelinoninflammatoryresponseinlungcontusion AT mertolgokce effectofghrelinoninflammatoryresponseinlungcontusion AT ozkansaydam effectofghrelinoninflammatoryresponseinlungcontusion AT muratcan effectofghrelinoninflammatoryresponseinlungcontusion AT sibelbektas effectofghrelinoninflammatoryresponseinlungcontusion AT serhanyurtlu effectofghrelinoninflammatoryresponseinlungcontusion |