Factors Affecting the Quality of Bacterial Genomes Assemblies by Canu after Nanopore Sequencing
Long-read sequencing (LRS), like Oxford Nanopore Technologies, is usually associated with higher error rates compared to previous generations. Factors affecting the assembly quality are the integrity of DNA, the flowcell efficiency, and, not least all, the raw data processing. Among LRS-intended de...
Main Authors: | Antonella Schiavone, Nicola Pugliese, Rossella Samarelli, Cosimo Cumbo, Crescenzio Francesco Minervini, Francesco Albano, Antonio Camarda |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-03-01
|
Series: | Applied Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3417/12/6/3110 |
Similar Items
-
Whole genome sequencing in the palm of your hand: how to implement a MinION Galaxy-based workflow in a food safety laboratory for rapid Salmonella spp. serotyping, virulence, and antimicrobial resistance gene identification
by: Alexandre Lamas, et al.
Published: (2023-12-01) -
Chloroplast Genome Draft of <i>Dryobalanops aromatica</i> Generated Using Oxford Nanopore Technology and Its Potential Application for Phylogenetic Study
by: Dwi Wahyuni, et al.
Published: (2021-11-01) -
ONT-Based Alternative Assemblies Impact on the Annotations of Unique versus Repetitive Features in the Genome of a Romanian Strain of <i>Drosophila melanogaster</i>
by: Alexandru Marian Bologa, et al.
Published: (2022-11-01) -
Mind the Gap: New Full-Length Sequences of <i>Blastocystis</i> Subtypes Generated via Oxford Nanopore Minion Sequencing Allow for Comparisons between Full-Length and Partial Sequences of the Small Subunit of the Ribosomal RNA Gene
by: Jenny G. Maloney, et al.
Published: (2021-05-01) -
Comparison of Reference-Based Assembly and De Novo Assembly for Bacterial Plasmid Reconstruction and AMR Gene Localization in <i>Salmonella enterica</i> Serovar Schwarzengrund Isolates
by: I-Chen Li, et al.
Published: (2022-01-01)