Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regeneration
Abstract Dental pulp regeneration is a promising strategy for addressing tooth disorders. Incorporating this strategy involves the fundamental challenge of establishing functional vascular networks using dental pulp stem cells (DPSCs) to support tissue regeneration. Current therapeutic approaches la...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-024-02393-9 |
| _version_ | 1797219568378183680 |
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| author | Xiaoling Wei Huaxing Xu Mengqi Zhou Qiangqiang Zhou Mingqiang Li Yuehua Liu |
| author_facet | Xiaoling Wei Huaxing Xu Mengqi Zhou Qiangqiang Zhou Mingqiang Li Yuehua Liu |
| author_sort | Xiaoling Wei |
| collection | DOAJ |
| description | Abstract Dental pulp regeneration is a promising strategy for addressing tooth disorders. Incorporating this strategy involves the fundamental challenge of establishing functional vascular networks using dental pulp stem cells (DPSCs) to support tissue regeneration. Current therapeutic approaches lack efficient and stable methods for activating DPSCs. In the study, we used a chemically modified microRNA (miRNA)-loaded tetrahedral-framework nucleic acid nanostructure to promote DPSC-mediated angiogenesis and dental pulp regeneration. Incorporating chemically modified miR-126-3p into tetrahedral DNA nanostructures (miR@TDNs) represents a notable advancement in the stability and efficacy of miRNA delivery into DPSCs. These nanostructures enhanced DPSC proliferation, migration, and upregulated angiogenesis-related genes, enhancing their paracrine signaling effects on endothelial cells. This enhanced effect was substantiated by improvements in endothelial cell tube formation, migration, and gene expression. Moreover, in vivo investigations employing matrigel plug assays and ectopic dental pulp transplantation confirmed the potential of miR@TDNs in promoting angiogenesis and facilitating dental pulp regeneration. Our findings demonstrated the potential of chemically modified miRNA-loaded nucleic acid nanostructures in enhancing DPSC-mediated angiogenesis and supporting dental pulp regeneration. These results highlighted the promising role of chemically modified nucleic acid-based delivery systems as therapeutic agents in regenerative dentistry and tissue engineering. Graphical abstract |
| first_indexed | 2024-04-24T12:35:43Z |
| format | Article |
| id | doaj.art-0621c507d2064267a4c85947a05fb28d |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-24T12:35:43Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-0621c507d2064267a4c85947a05fb28d2024-04-07T11:29:14ZengBMCJournal of Nanobiotechnology1477-31552024-04-0122111810.1186/s12951-024-02393-9Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regenerationXiaoling Wei0Huaxing Xu1Mengqi Zhou2Qiangqiang Zhou3Mingqiang Li4Yuehua Liu5Shanghai Stomatological Hospital and School of Stomatology, Fudan UniversityShanghai Stomatological Hospital and School of Stomatology, Fudan UniversityShanghai Stomatological Hospital and School of Stomatology, Fudan UniversityShanghai Stomatological Hospital and School of Stomatology, Fudan UniversitySchool of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, National Center for Translational Medicine, Shanghai Jiao Tong UniversityShanghai Stomatological Hospital and School of Stomatology, Fudan UniversityAbstract Dental pulp regeneration is a promising strategy for addressing tooth disorders. Incorporating this strategy involves the fundamental challenge of establishing functional vascular networks using dental pulp stem cells (DPSCs) to support tissue regeneration. Current therapeutic approaches lack efficient and stable methods for activating DPSCs. In the study, we used a chemically modified microRNA (miRNA)-loaded tetrahedral-framework nucleic acid nanostructure to promote DPSC-mediated angiogenesis and dental pulp regeneration. Incorporating chemically modified miR-126-3p into tetrahedral DNA nanostructures (miR@TDNs) represents a notable advancement in the stability and efficacy of miRNA delivery into DPSCs. These nanostructures enhanced DPSC proliferation, migration, and upregulated angiogenesis-related genes, enhancing their paracrine signaling effects on endothelial cells. This enhanced effect was substantiated by improvements in endothelial cell tube formation, migration, and gene expression. Moreover, in vivo investigations employing matrigel plug assays and ectopic dental pulp transplantation confirmed the potential of miR@TDNs in promoting angiogenesis and facilitating dental pulp regeneration. Our findings demonstrated the potential of chemically modified miRNA-loaded nucleic acid nanostructures in enhancing DPSC-mediated angiogenesis and supporting dental pulp regeneration. These results highlighted the promising role of chemically modified nucleic acid-based delivery systems as therapeutic agents in regenerative dentistry and tissue engineering. Graphical abstracthttps://doi.org/10.1186/s12951-024-02393-9Dental pulp regenerationDental pulp stem cellsAngiogenesisNucleic acid modificationsTetrahedral-framework nucleic acid nanostructure |
| spellingShingle | Xiaoling Wei Huaxing Xu Mengqi Zhou Qiangqiang Zhou Mingqiang Li Yuehua Liu Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regeneration Journal of Nanobiotechnology Dental pulp regeneration Dental pulp stem cells Angiogenesis Nucleic acid modifications Tetrahedral-framework nucleic acid nanostructure |
| title | Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regeneration |
| title_full | Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regeneration |
| title_fullStr | Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regeneration |
| title_full_unstemmed | Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regeneration |
| title_short | Chemically modified microRNA delivery via DNA tetrahedral frameworks for dental pulp regeneration |
| title_sort | chemically modified microrna delivery via dna tetrahedral frameworks for dental pulp regeneration |
| topic | Dental pulp regeneration Dental pulp stem cells Angiogenesis Nucleic acid modifications Tetrahedral-framework nucleic acid nanostructure |
| url | https://doi.org/10.1186/s12951-024-02393-9 |
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