Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells
The anti-inflammatory cytokine interleukin-37 (IL-37) plays a key role in inhibiting innate and adaptive immunity. Past results have shown that IL-37 is elevated in human Treg cells compared to other T cell subsets and contributes to enhancing the Treg transcription factor, forkhead box protein P3 (...
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MDPI AG
2022-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/16/2565 |
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| author | Douglas Grant Osborne Joanne Domenico Mayumi Fujita |
| author_facet | Douglas Grant Osborne Joanne Domenico Mayumi Fujita |
| author_sort | Douglas Grant Osborne |
| collection | DOAJ |
| description | The anti-inflammatory cytokine interleukin-37 (IL-37) plays a key role in inhibiting innate and adaptive immunity. Past results have shown that IL-37 is elevated in human Treg cells compared to other T cell subsets and contributes to enhancing the Treg transcription factor, forkhead box protein P3 (FOXP3). However, it is unknown if ectopic expression of IL-37 in non-Treg CD4+ T cells can lead to the development of Treg phenotype and function. In the present study, we used a PrimeFlow<sup>®</sup> RNA assay and confirmed elevated <i>IL37</i> expression in human Treg cells. We then stably transfected the non-Treg CD4+ T cell leukemia cell line, E6 Jurkat cells, with <i>IL37</i> and found significant induction of the Treg phenotype. These IL-37-expressing Jurkat cells had elevated CTLA-4 and FOXP3 and produced IL-10. In conjunction with the Treg phenotype, IL-37-expressing Jurkat cells suppressed T cell activation/proliferation, comparable to human primary Treg cells. The creation of this stable human Treg-like cell line has the potential to provide further assistance for in vitro studies of human Treg cells, as it is more convenient than the use of primary human Treg cells. Furthermore, it provides insights into Treg cell biology and function. |
| first_indexed | 2024-03-09T13:40:57Z |
| format | Article |
| id | doaj.art-06283a098eac4df190b423159985f037 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T13:40:57Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-06283a098eac4df190b423159985f0372023-11-30T21:06:56ZengMDPI AGCells2073-44092022-08-011116256510.3390/cells11162565Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat CellsDouglas Grant Osborne0Joanne Domenico1Mayumi Fujita2Department of Dermatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Dermatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Dermatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USAThe anti-inflammatory cytokine interleukin-37 (IL-37) plays a key role in inhibiting innate and adaptive immunity. Past results have shown that IL-37 is elevated in human Treg cells compared to other T cell subsets and contributes to enhancing the Treg transcription factor, forkhead box protein P3 (FOXP3). However, it is unknown if ectopic expression of IL-37 in non-Treg CD4+ T cells can lead to the development of Treg phenotype and function. In the present study, we used a PrimeFlow<sup>®</sup> RNA assay and confirmed elevated <i>IL37</i> expression in human Treg cells. We then stably transfected the non-Treg CD4+ T cell leukemia cell line, E6 Jurkat cells, with <i>IL37</i> and found significant induction of the Treg phenotype. These IL-37-expressing Jurkat cells had elevated CTLA-4 and FOXP3 and produced IL-10. In conjunction with the Treg phenotype, IL-37-expressing Jurkat cells suppressed T cell activation/proliferation, comparable to human primary Treg cells. The creation of this stable human Treg-like cell line has the potential to provide further assistance for in vitro studies of human Treg cells, as it is more convenient than the use of primary human Treg cells. Furthermore, it provides insights into Treg cell biology and function.https://www.mdpi.com/2073-4409/11/16/2565IL-37regulatory T cellTregFOXP3Jurkat |
| spellingShingle | Douglas Grant Osborne Joanne Domenico Mayumi Fujita Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells Cells IL-37 regulatory T cell Treg FOXP3 Jurkat |
| title | Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells |
| title_full | Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells |
| title_fullStr | Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells |
| title_full_unstemmed | Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells |
| title_short | Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells |
| title_sort | expression of il 37 induces a regulatory t cell like phenotype and function in jurkat cells |
| topic | IL-37 regulatory T cell Treg FOXP3 Jurkat |
| url | https://www.mdpi.com/2073-4409/11/16/2565 |
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