Metabolomic analysis of three Mollicute species.
We present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089312&type=printable |
| _version_ | 1826799120337076224 |
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| author | Anna A Vanyushkina Gleb Y Fisunov Alexey Y Gorbachev Dmitri E Kamashev Vadim M Govorun |
| author_facet | Anna A Vanyushkina Gleb Y Fisunov Alexey Y Gorbachev Dmitri E Kamashev Vadim M Govorun |
| author_sort | Anna A Vanyushkina |
| collection | DOAJ |
| description | We present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation to environmental conditions in the three species, we analyzed the metabolome of these bacteria. Metabolic pathways were reconstructed using the proteogenomic annotation data provided by our lab. The results of metabolome, proteome and genome profiling suggest a fundamental difference in the adaptation of the three closely related Mollicute species to stress conditions. As the transaldolase is not annotated in Mollicutes, we propose variants of the pentose phosphate pathway catalyzed by annotated enzymes for three species. For metabolite detection we employed high performance liquid chromatography coupled with mass spectrometry. We used liquid chromatography method - hydrophilic interaction chromatography with silica column - as it effectively separates highly polar cellular metabolites prior to their detection by mass spectrometer. |
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| format | Article |
| id | doaj.art-0629c14bc11f43e7b2cc4acc4e78133c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-17T00:30:02Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0629c14bc11f43e7b2cc4acc4e78133c2025-02-21T05:36:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e8931210.1371/journal.pone.0089312Metabolomic analysis of three Mollicute species.Anna A VanyushkinaGleb Y FisunovAlexey Y GorbachevDmitri E KamashevVadim M GovorunWe present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation to environmental conditions in the three species, we analyzed the metabolome of these bacteria. Metabolic pathways were reconstructed using the proteogenomic annotation data provided by our lab. The results of metabolome, proteome and genome profiling suggest a fundamental difference in the adaptation of the three closely related Mollicute species to stress conditions. As the transaldolase is not annotated in Mollicutes, we propose variants of the pentose phosphate pathway catalyzed by annotated enzymes for three species. For metabolite detection we employed high performance liquid chromatography coupled with mass spectrometry. We used liquid chromatography method - hydrophilic interaction chromatography with silica column - as it effectively separates highly polar cellular metabolites prior to their detection by mass spectrometer.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089312&type=printable |
| spellingShingle | Anna A Vanyushkina Gleb Y Fisunov Alexey Y Gorbachev Dmitri E Kamashev Vadim M Govorun Metabolomic analysis of three Mollicute species. PLoS ONE |
| title | Metabolomic analysis of three Mollicute species. |
| title_full | Metabolomic analysis of three Mollicute species. |
| title_fullStr | Metabolomic analysis of three Mollicute species. |
| title_full_unstemmed | Metabolomic analysis of three Mollicute species. |
| title_short | Metabolomic analysis of three Mollicute species. |
| title_sort | metabolomic analysis of three mollicute species |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089312&type=printable |
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