Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani
Potato early blight (PEB), a foliar disease of potato during the growing period, caused by Alternaria sp., is common in major potato-producing areas worldwide. Effective agents to control this disease or completely resistant potato varieties are absent. Large-scale use of fungicides is limited due t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1357579/full |
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| author | Qing Li Qing Li Yan Feng Jianmei Li Jianmei Li Yang Hai Liping Si Chen Tan Jing Peng Zuo Hu Zhou Li Canhui Li Canhui Li Dahai Hao Dahai Hao Wei Tang Wei Tang |
| author_facet | Qing Li Qing Li Yan Feng Jianmei Li Jianmei Li Yang Hai Liping Si Chen Tan Jing Peng Zuo Hu Zhou Li Canhui Li Canhui Li Dahai Hao Dahai Hao Wei Tang Wei Tang |
| author_sort | Qing Li |
| collection | DOAJ |
| description | Potato early blight (PEB), a foliar disease of potato during the growing period, caused by Alternaria sp., is common in major potato-producing areas worldwide. Effective agents to control this disease or completely resistant potato varieties are absent. Large-scale use of fungicides is limited due to possibility of increase in pathogen resistance and the requirements of ecological agriculture. In this study, we focused on the composition and infection characteristics of early blight pathogens in Yunnan Province and screened candidate pathogenesis-related pathways and genes. We isolated 85 strains of Alternaria sp. fungi from typical early blight spots in three potato-growing regions in Yunnan Province from 2018 to 2022, and identified 35 strains of Alternaria solani and 50 strains of Alternaria alternata by morphological characterization and ITS sequence comparison, which were identified as the main and conditional pathogens causing early blight in potato, respectively. Scanning electron microscope analysis confirmed only A. solani producing appressorium at 4 h after inoculation successfully infected the leaf cells. Via genome assembly and annotation, combine transcriptome and proteomic analysis, the following pathogenicity-related unit, transcription factors and metabolic pathway were identified: (1) cell wall-degrading enzymes, such as pectinase, keratinase, and cellulase; (2) genes and pathways related to conidia germination and pathogenicity, such as ubiquitination and peroxisomes; and (3) transcription factors, such as Zn-clus, C2H2, bZIP, and bHLH. These elements were responsible for PEB epidemic in Yunnan. |
| first_indexed | 2024-04-25T01:00:05Z |
| format | Article |
| id | doaj.art-062b6e3d14fa40e2ba098d193a334062 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-25T01:00:05Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-062b6e3d14fa40e2ba098d193a3340622024-03-11T04:31:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13575791357579Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solaniQing Li0Qing Li1Yan Feng2Jianmei Li3Jianmei Li4Yang Hai5Liping Si6Chen Tan7Jing Peng8Zuo Hu9Zhou Li10Canhui Li11Canhui Li12Dahai Hao13Dahai Hao14Wei Tang15Wei Tang16Yunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming, ChinaSchool of Economics and Management, Yunnan Normal University, Kunming, ChinaYunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming, ChinaYunnan YinMore Modern Agriculture Co., Ltd., Kunming, ChinaYunnan YinMore Modern Agriculture Co., Ltd., Kunming, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming, ChinaZhaotong Academy of Agricultural Sciences, Zhaotong, ChinaZhaotong Academy of Agricultural Sciences, Zhaotong, ChinaYunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming, ChinaYunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming, ChinaYunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming, ChinaPotato early blight (PEB), a foliar disease of potato during the growing period, caused by Alternaria sp., is common in major potato-producing areas worldwide. Effective agents to control this disease or completely resistant potato varieties are absent. Large-scale use of fungicides is limited due to possibility of increase in pathogen resistance and the requirements of ecological agriculture. In this study, we focused on the composition and infection characteristics of early blight pathogens in Yunnan Province and screened candidate pathogenesis-related pathways and genes. We isolated 85 strains of Alternaria sp. fungi from typical early blight spots in three potato-growing regions in Yunnan Province from 2018 to 2022, and identified 35 strains of Alternaria solani and 50 strains of Alternaria alternata by morphological characterization and ITS sequence comparison, which were identified as the main and conditional pathogens causing early blight in potato, respectively. Scanning electron microscope analysis confirmed only A. solani producing appressorium at 4 h after inoculation successfully infected the leaf cells. Via genome assembly and annotation, combine transcriptome and proteomic analysis, the following pathogenicity-related unit, transcription factors and metabolic pathway were identified: (1) cell wall-degrading enzymes, such as pectinase, keratinase, and cellulase; (2) genes and pathways related to conidia germination and pathogenicity, such as ubiquitination and peroxisomes; and (3) transcription factors, such as Zn-clus, C2H2, bZIP, and bHLH. These elements were responsible for PEB epidemic in Yunnan.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1357579/fullpotato early blightAlternaria solaniAlternaria alternatagenometranscriptomeproteome |
| spellingShingle | Qing Li Qing Li Yan Feng Jianmei Li Jianmei Li Yang Hai Liping Si Chen Tan Jing Peng Zuo Hu Zhou Li Canhui Li Canhui Li Dahai Hao Dahai Hao Wei Tang Wei Tang Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani Frontiers in Microbiology potato early blight Alternaria solani Alternaria alternata genome transcriptome proteome |
| title | Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani |
| title_full | Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani |
| title_fullStr | Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani |
| title_full_unstemmed | Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani |
| title_short | Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani |
| title_sort | multi omics approaches to understand pathogenicity during potato early blight disease caused by alternaria solani |
| topic | potato early blight Alternaria solani Alternaria alternata genome transcriptome proteome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1357579/full |
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