Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease
Monocytes are classified according to their CD14 and CD16 expression into classical (reparative), intermediate (inflammatory), and non-classical. This study assessed the frequency of monocyte and the relationship between monocyte subset percentages and the levels of blood cytokines in Colombian chag...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01671/full |
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| author | Sergio Gómez-Olarte Sergio Gómez-Olarte Natalia I. Bolaños Mariana Echeverry Ayda N. Rodríguez Adriana Cuéllar Concepción J. Puerta Alejandro Mariño John M. González |
| author_facet | Sergio Gómez-Olarte Sergio Gómez-Olarte Natalia I. Bolaños Mariana Echeverry Ayda N. Rodríguez Adriana Cuéllar Concepción J. Puerta Alejandro Mariño John M. González |
| author_sort | Sergio Gómez-Olarte |
| collection | DOAJ |
| description | Monocytes are classified according to their CD14 and CD16 expression into classical (reparative), intermediate (inflammatory), and non-classical. This study assessed the frequency of monocyte and the relationship between monocyte subset percentages and the levels of blood cytokines in Colombian chagasic patients with different clinical forms. This study included chagasic patients in different clinical stages: indeterminate (IND) n = 14, chronic chagasic cardiomyopathy (CCC) n = 14, and heart transplant chagasic (HTCC) n = 9; controls with non-chagasic cardiopathy (NCC) n = 15, and healthy individuals (HI) n = 15. Peripheral blood mononuclear cells (PBMCs) were isolated, labeled for CD14, CD16, and HLA-DR, and analyzed by flow cytometry. Cytokines were measured with a bead-based immunoassay. Percentages of total CD14+ CD16+ and CD14+ HLA-DR+ monocytes were higher in patients with heart involvement (CCC, HTCC, and NCC) than controls. Percentages of intermediate monocytes increased in symptomatic chagasic patients (CCC and HTCC) compared to asymptomatic chagasic patients (IND) and controls (HI). Asymptomatic chagasic patients (IND) had higher percentages of classical monocytes, an increased production of CCL17 chemokine compared to chagasic symptomatic patients (CCC), and their levels of CCL17 was positively correlated with the percentage of classical monocyte subset. In CCC, the percentages of intermediate and classical monocytes were positively correlated with IL-6 levels, which were higher in this group compared to HI, and negatively with IL-12p40 concentration, respectively. Remarkably, there also was an important increased of classical monocytes frequency in three chronic chagasic patients who underwent cardiac transplant, of which one received anti-parasitic treatment. Our findings suggest that cardiac chagasic patients have an increased percentage of inflammatory monocytes and produce more IL-6, a biomarker of heart failure and left ventricular dysfunction, whereas asymptomatic chagasic individuals present a higher percentage of reparative monocytes and CCL17. |
| first_indexed | 2024-12-11T10:31:30Z |
| format | Article |
| id | doaj.art-062c3b593a824ccea756b01205cea11b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T10:31:30Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-062c3b593a824ccea756b01205cea11b2022-12-22T01:10:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-07-011010.3389/fimmu.2019.01671448309Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas DiseaseSergio Gómez-Olarte0Sergio Gómez-Olarte1Natalia I. Bolaños2Mariana Echeverry3Ayda N. Rodríguez4Adriana Cuéllar5Concepción J. Puerta6Alejandro Mariño7John M. González8Grupo de Ciencias Básicas Médicas, School of Medicine, Universidad de los Andes, Bogotá, ColombiaDepartment of Biological Sciences, School of Sciences, Universidad de los Andes, Bogotá, ColombiaGrupo de Ciencias Básicas Médicas, School of Medicine, Universidad de los Andes, Bogotá, ColombiaGrupo de Ciencias Básicas Médicas, School of Medicine, Universidad de los Andes, Bogotá, ColombiaNational Blood Bank, Colombian Red Cross, Bogotá, ColombiaGrupo de Ciencias del Laboratorio Clínico, School of Sciences, Pontificia Universidad Javeriana, Bogotá, ColombiaLaboratorio de Parasitología Molecular, School of Sciences, Pontificia Universidad Javeriana, Bogotá, ColombiaFailure and Heart Transplantation Clinic, Hospital Universitario San Ignacio, Bogotá, ColombiaGrupo de Ciencias Básicas Médicas, School of Medicine, Universidad de los Andes, Bogotá, ColombiaMonocytes are classified according to their CD14 and CD16 expression into classical (reparative), intermediate (inflammatory), and non-classical. This study assessed the frequency of monocyte and the relationship between monocyte subset percentages and the levels of blood cytokines in Colombian chagasic patients with different clinical forms. This study included chagasic patients in different clinical stages: indeterminate (IND) n = 14, chronic chagasic cardiomyopathy (CCC) n = 14, and heart transplant chagasic (HTCC) n = 9; controls with non-chagasic cardiopathy (NCC) n = 15, and healthy individuals (HI) n = 15. Peripheral blood mononuclear cells (PBMCs) were isolated, labeled for CD14, CD16, and HLA-DR, and analyzed by flow cytometry. Cytokines were measured with a bead-based immunoassay. Percentages of total CD14+ CD16+ and CD14+ HLA-DR+ monocytes were higher in patients with heart involvement (CCC, HTCC, and NCC) than controls. Percentages of intermediate monocytes increased in symptomatic chagasic patients (CCC and HTCC) compared to asymptomatic chagasic patients (IND) and controls (HI). Asymptomatic chagasic patients (IND) had higher percentages of classical monocytes, an increased production of CCL17 chemokine compared to chagasic symptomatic patients (CCC), and their levels of CCL17 was positively correlated with the percentage of classical monocyte subset. In CCC, the percentages of intermediate and classical monocytes were positively correlated with IL-6 levels, which were higher in this group compared to HI, and negatively with IL-12p40 concentration, respectively. Remarkably, there also was an important increased of classical monocytes frequency in three chronic chagasic patients who underwent cardiac transplant, of which one received anti-parasitic treatment. Our findings suggest that cardiac chagasic patients have an increased percentage of inflammatory monocytes and produce more IL-6, a biomarker of heart failure and left ventricular dysfunction, whereas asymptomatic chagasic individuals present a higher percentage of reparative monocytes and CCL17.https://www.frontiersin.org/article/10.3389/fimmu.2019.01671/fullcardiomyopathyChagas diseasechemokinescytokinesmonocytesinnate immunity |
| spellingShingle | Sergio Gómez-Olarte Sergio Gómez-Olarte Natalia I. Bolaños Mariana Echeverry Ayda N. Rodríguez Adriana Cuéllar Concepción J. Puerta Alejandro Mariño John M. González Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease Frontiers in Immunology cardiomyopathy Chagas disease chemokines cytokines monocytes innate immunity |
| title | Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease |
| title_full | Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease |
| title_fullStr | Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease |
| title_full_unstemmed | Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease |
| title_short | Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease |
| title_sort | intermediate monocytes and cytokine production associated with severe forms of chagas disease |
| topic | cardiomyopathy Chagas disease chemokines cytokines monocytes innate immunity |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01671/full |
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