Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male)
The hybrid loach of M. anguillicaudatus (female) and M. bipartitus (male) have the desirable trait of growth performance. Recently, farming scale of this hybrid has been gradually increased in Asia, suggesting a promise of a new variety for loach. In this study, the complete mitochondrial genome of...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Mitochondrial DNA. Part B. Resources |
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| Online Access: | http://dx.doi.org/10.1080/23802359.2019.1566788 |
| _version_ | 1827774024271265792 |
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| author | Dechang Chu Hongmei Zhang Xiaodong Li Huimin Jiang Jixin Lv Chenchen Shao Linying Mu Xiaoyu Wang Yanan Wang Zhenxu Deng |
| author_facet | Dechang Chu Hongmei Zhang Xiaodong Li Huimin Jiang Jixin Lv Chenchen Shao Linying Mu Xiaoyu Wang Yanan Wang Zhenxu Deng |
| author_sort | Dechang Chu |
| collection | DOAJ |
| description | The hybrid loach of M. anguillicaudatus (female) and M. bipartitus (male) have the desirable trait of growth performance. Recently, farming scale of this hybrid has been gradually increased in Asia, suggesting a promise of a new variety for loach. In this study, the complete mitochondrial genome of the hybrid loach was obtained by PCR. The genome is 16,647 bp in length, including 2 ribosomal RNA genes. 13 protein-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition is 29.75% for A, 28.28% for T, 25.61% for C, and 16.36% for G. The phylogenetic tree showed that Misgurnus family got together for one branch, which includes this hybrid loach, and the other loaches had their own branches. Also, the mitochondrial genome sequence of the hybrid loach was aligned by BLAST, compared with Cobitinae the sequence similarity could reach >95%, and the similarity to Misgurnus was >99%. The hybrid loach follows the matrilineal inheritance. |
| first_indexed | 2024-03-11T13:33:02Z |
| format | Article |
| id | doaj.art-062dc0ce77f2489cb44aa567e7ab42d0 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:33:02Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-062dc0ce77f2489cb44aa567e7ab42d02023-11-02T16:07:25ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592019-01-014177978010.1080/23802359.2019.15667881566788Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male)Dechang Chu0Hongmei Zhang1Xiaodong Li2Huimin Jiang3Jixin Lv4Chenchen Shao5Linying Mu6Xiaoyu Wang7Yanan Wang8Zhenxu Deng9Heze UniversityHeze UniversityHechi UniversityHeze UniversityHeze UniversityHeze UniversityHeze UniversityHeze UniversityHeze UniversityHeze UniversityThe hybrid loach of M. anguillicaudatus (female) and M. bipartitus (male) have the desirable trait of growth performance. Recently, farming scale of this hybrid has been gradually increased in Asia, suggesting a promise of a new variety for loach. In this study, the complete mitochondrial genome of the hybrid loach was obtained by PCR. The genome is 16,647 bp in length, including 2 ribosomal RNA genes. 13 protein-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition is 29.75% for A, 28.28% for T, 25.61% for C, and 16.36% for G. The phylogenetic tree showed that Misgurnus family got together for one branch, which includes this hybrid loach, and the other loaches had their own branches. Also, the mitochondrial genome sequence of the hybrid loach was aligned by BLAST, compared with Cobitinae the sequence similarity could reach >95%, and the similarity to Misgurnus was >99%. The hybrid loach follows the matrilineal inheritance.http://dx.doi.org/10.1080/23802359.2019.1566788hybrid loachmisgurnusmitogenomeconservation |
| spellingShingle | Dechang Chu Hongmei Zhang Xiaodong Li Huimin Jiang Jixin Lv Chenchen Shao Linying Mu Xiaoyu Wang Yanan Wang Zhenxu Deng Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male) Mitochondrial DNA. Part B. Resources hybrid loach misgurnus mitogenome conservation |
| title | Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male) |
| title_full | Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male) |
| title_fullStr | Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male) |
| title_full_unstemmed | Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male) |
| title_short | Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and M. bipartitus (male) |
| title_sort | molecular characteristics of mitochondrial dna and phylogenetic analysis of the hybrid loach of misgurnus anguillicaudatus female and m bipartitus male |
| topic | hybrid loach misgurnus mitogenome conservation |
| url | http://dx.doi.org/10.1080/23802359.2019.1566788 |
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