Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows
The present study examined whether priming with estradiol benzoate (EB) for 12 h increased both the peak and duration of LH release in response to kisspeptin (KISS1, KP) in cows. In a Latin square design, ovariectomized Nelore cows (<i>n</i> = 8) received: Control, i.m. 4 mL of 0.9% sali...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/5/1236 |
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| author | Gustavo Guerino Macedo Emiliana de Oliveira Santana Batista Gustavo Martins Gomes dos Santos Michael J. D’Occhio Pietro Sampaio Baruselli |
| author_facet | Gustavo Guerino Macedo Emiliana de Oliveira Santana Batista Gustavo Martins Gomes dos Santos Michael J. D’Occhio Pietro Sampaio Baruselli |
| author_sort | Gustavo Guerino Macedo |
| collection | DOAJ |
| description | The present study examined whether priming with estradiol benzoate (EB) for 12 h increased both the peak and duration of LH release in response to kisspeptin (KISS1, KP) in cows. In a Latin square design, ovariectomized Nelore cows (<i>n</i> = 8) received: Control, i.m. 4 mL of 0.9% saline; KP, i.m. 4 mg murine KISS1-10; EBKP, i.m. 4 mg KISS1-10 + i.m. 2 mg EB simultaneously; EB12KP, i.m. 4 mg KISS1-10 + i.m. 2 mg EB 12 h before KISS1-10. Concentrations of LH were determined in blood samples obtained at time 0 (treatment), 20, 40, 60, 90, 120, 150, 180, 210 and 270 min. Concentrations of LH were analyzed by Proc GLIMMIX for repeated measures. In case of significance, the adjusted Tukey test was used to test for differences among treatments. GraphPad 8.0 PRISM<sup>®</sup> was used to determine the area under the LH-response curve (AUC) after injection of KISS1-10. Plasma LH remained relatively constant throughout sampling after treatment with saline. The peak in LH after injection of KISS1-10 occurred at 20 min in Groups KP and EBKP and at 40 min in Group EB12KP. The peak LH response (∆LH, ng/mL) was greater (<i>p</i> < 0.01) in Group EB12KP (5.6 ± 0.9) than in Groups KP (2.4 ± 0.9) and EBKP (3.5 ± 0.9), which did not differ. AUC (LH ng/mL*min) was greater (<i>p</i> = 0.02) in Group EB12KP (439 ± 73) than in Groups KP (176 ± 73) and EBKP (241 ± 73), with the latter two groups not differing. The findings indicated that 12 h priming with EB increased both the peak and duration of the LH response to treatment with KISS1. The incorporation of EB priming and KISS1 could improve the efficiency of estrus synchronization with fixed-time AI in cows. This would have an important practical application in assisted breeding in beef and dairy cattle. |
| first_indexed | 2024-03-10T11:58:32Z |
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| spelling | doaj.art-062dfd27d5894c259de7dd74c4dfb9f62023-11-21T17:03:38ZengMDPI AGAnimals2076-26152021-04-01115123610.3390/ani11051236Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized CowsGustavo Guerino Macedo0Emiliana de Oliveira Santana Batista1Gustavo Martins Gomes dos Santos2Michael J. D’Occhio3Pietro Sampaio Baruselli4Faculty of Veterinary Medicine and Animal Sciences, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Mato Grosso do Sul, BrazilFaculty of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo 05508-270, Sao Paulo, BrazilSheepembryo, Londrina 80010-000, Parana, BrazilFaculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, AustraliaFaculty of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo 05508-270, Sao Paulo, BrazilThe present study examined whether priming with estradiol benzoate (EB) for 12 h increased both the peak and duration of LH release in response to kisspeptin (KISS1, KP) in cows. In a Latin square design, ovariectomized Nelore cows (<i>n</i> = 8) received: Control, i.m. 4 mL of 0.9% saline; KP, i.m. 4 mg murine KISS1-10; EBKP, i.m. 4 mg KISS1-10 + i.m. 2 mg EB simultaneously; EB12KP, i.m. 4 mg KISS1-10 + i.m. 2 mg EB 12 h before KISS1-10. Concentrations of LH were determined in blood samples obtained at time 0 (treatment), 20, 40, 60, 90, 120, 150, 180, 210 and 270 min. Concentrations of LH were analyzed by Proc GLIMMIX for repeated measures. In case of significance, the adjusted Tukey test was used to test for differences among treatments. GraphPad 8.0 PRISM<sup>®</sup> was used to determine the area under the LH-response curve (AUC) after injection of KISS1-10. Plasma LH remained relatively constant throughout sampling after treatment with saline. The peak in LH after injection of KISS1-10 occurred at 20 min in Groups KP and EBKP and at 40 min in Group EB12KP. The peak LH response (∆LH, ng/mL) was greater (<i>p</i> < 0.01) in Group EB12KP (5.6 ± 0.9) than in Groups KP (2.4 ± 0.9) and EBKP (3.5 ± 0.9), which did not differ. AUC (LH ng/mL*min) was greater (<i>p</i> = 0.02) in Group EB12KP (439 ± 73) than in Groups KP (176 ± 73) and EBKP (241 ± 73), with the latter two groups not differing. The findings indicated that 12 h priming with EB increased both the peak and duration of the LH response to treatment with KISS1. The incorporation of EB priming and KISS1 could improve the efficiency of estrus synchronization with fixed-time AI in cows. This would have an important practical application in assisted breeding in beef and dairy cattle.https://www.mdpi.com/2076-2615/11/5/1236estradiol primingkisspeptinLHfixed-time AIcow |
| spellingShingle | Gustavo Guerino Macedo Emiliana de Oliveira Santana Batista Gustavo Martins Gomes dos Santos Michael J. D’Occhio Pietro Sampaio Baruselli Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows Animals estradiol priming kisspeptin LH fixed-time AI cow |
| title | Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows |
| title_full | Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows |
| title_fullStr | Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows |
| title_full_unstemmed | Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows |
| title_short | Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows |
| title_sort | estradiol priming potentiates the kisspeptin induced release of lh in ovariectomized cows |
| topic | estradiol priming kisspeptin LH fixed-time AI cow |
| url | https://www.mdpi.com/2076-2615/11/5/1236 |
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