Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication
Summary: The urothelium is an epithelia barrier lined by a luminal layer of binucleated, octoploid, superficial cells. Superficial cells are critical for production and transport of uroplakins, a family of proteins that assemble into a waterproof crystalline plaque that helps protect against infecti...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-10-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718314876 |
| _version_ | 1819147006560436224 |
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| author | Jia Wang Ekatherina Batourina Kerry Schneider Spenser Souza Theresa Swayne Chang Liu Christopher D. George Tiffany Tate Hanbin Dan Gregory Wiessner Yelena Zhuravlev Julie C. Canman Indira U. Mysorekar Cathy Lee Mendelsohn |
| author_facet | Jia Wang Ekatherina Batourina Kerry Schneider Spenser Souza Theresa Swayne Chang Liu Christopher D. George Tiffany Tate Hanbin Dan Gregory Wiessner Yelena Zhuravlev Julie C. Canman Indira U. Mysorekar Cathy Lee Mendelsohn |
| author_sort | Jia Wang |
| collection | DOAJ |
| description | Summary: The urothelium is an epithelia barrier lined by a luminal layer of binucleated, octoploid, superficial cells. Superficial cells are critical for production and transport of uroplakins, a family of proteins that assemble into a waterproof crystalline plaque that helps protect against infection and toxic substances. Adult urothelium is nearly quiescent, but rapidly regenerates in response to injury. Yet the mechanism by which binucleated, polyploid, superficial cells are produced remains unclear. Here, we show that superficial cells are likely to be derived from a population of binucleated intermediate cells, which are produced from mononucleated intermediate cells via incomplete cytokinesis. We show that binucleated intermediate and superficial cells increase DNA content via endoreplication, passing through S phase without entering mitosis. The urothelium can be permanently damaged by repetitive or chronic injury or disease. Identification of the mechanism by which superficial cells are produced may be important for developing strategies for urothelial repair. : Binucleated superficial cells are critical for urothelial barrier function. Wang et al. show that they derive from binucleated intermediate cells that form via incomplete cytokinesis. Both superficial and intermediate cells increase ploidy via endoreplication, a feature likely to be important for repair and response to environmental changes. Keywords: endoreplication, urothelium, polyploidy, epithelial barrier, regeneration |
| first_indexed | 2024-12-22T13:22:57Z |
| format | Article |
| id | doaj.art-0631302d35814fb0a9465947e9905826 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-22T13:22:57Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-0631302d35814fb0a9465947e99058262022-12-21T18:24:24ZengElsevierCell Reports2211-12472018-10-01252464477.e4Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and EndoreplicationJia Wang0Ekatherina Batourina1Kerry Schneider2Spenser Souza3Theresa Swayne4Chang Liu5Christopher D. George6Tiffany Tate7Hanbin Dan8Gregory Wiessner9Yelena Zhuravlev10Julie C. Canman11Indira U. Mysorekar12Cathy Lee Mendelsohn13Department of Urology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USADepartment of Urology, Genetics, and Development and Pathology and Cell Biology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USACollege of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USATulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA 70112, USAConfocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USADepartment of Urology, Genetics, and Development and Pathology and Cell Biology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USADepartment of Urology, Genetics, and Development and Pathology and Cell Biology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USADepartment of Urology, Genetics, and Development and Pathology and Cell Biology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USADepartment of Systems Biology, Columbia University, New York, NY 10032, USADepartment of Urology, Genetics, and Development and Pathology and Cell Biology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USADepartment of Urology, Genetics, and Development and Pathology and Cell Biology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USADepartment of Pathology and Cell Biology, Columbia University, 630 West 168th Street, New York, NY 10032, USADepartments of Obstetrics and Gynecology and Pathology and Immunology and Center for Reproductive Sciences, Washington University School of Medicine, St. Louis, MO 63110, USADepartment of Urology, Genetics, and Development and Pathology and Cell Biology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USA; Corresponding authorSummary: The urothelium is an epithelia barrier lined by a luminal layer of binucleated, octoploid, superficial cells. Superficial cells are critical for production and transport of uroplakins, a family of proteins that assemble into a waterproof crystalline plaque that helps protect against infection and toxic substances. Adult urothelium is nearly quiescent, but rapidly regenerates in response to injury. Yet the mechanism by which binucleated, polyploid, superficial cells are produced remains unclear. Here, we show that superficial cells are likely to be derived from a population of binucleated intermediate cells, which are produced from mononucleated intermediate cells via incomplete cytokinesis. We show that binucleated intermediate and superficial cells increase DNA content via endoreplication, passing through S phase without entering mitosis. The urothelium can be permanently damaged by repetitive or chronic injury or disease. Identification of the mechanism by which superficial cells are produced may be important for developing strategies for urothelial repair. : Binucleated superficial cells are critical for urothelial barrier function. Wang et al. show that they derive from binucleated intermediate cells that form via incomplete cytokinesis. Both superficial and intermediate cells increase ploidy via endoreplication, a feature likely to be important for repair and response to environmental changes. Keywords: endoreplication, urothelium, polyploidy, epithelial barrier, regenerationhttp://www.sciencedirect.com/science/article/pii/S2211124718314876 |
| spellingShingle | Jia Wang Ekatherina Batourina Kerry Schneider Spenser Souza Theresa Swayne Chang Liu Christopher D. George Tiffany Tate Hanbin Dan Gregory Wiessner Yelena Zhuravlev Julie C. Canman Indira U. Mysorekar Cathy Lee Mendelsohn Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication Cell Reports |
| title | Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication |
| title_full | Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication |
| title_fullStr | Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication |
| title_full_unstemmed | Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication |
| title_short | Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication |
| title_sort | polyploid superficial cells that maintain the urothelial barrier are produced via incomplete cytokinesis and endoreplication |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718314876 |
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