Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli
The aim of this study was to investigate the electric field frequency and the time of exposure that causes maximum inhibition of Escherichia coli (E. coli) growth. Methods and Results: Bacterial suspensions were subjected to an extremely low frequency electric field (ELFEF) with a 0.1 Hz interval...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2022-06-01
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| Series: | International Journal of Biomedicine |
| Subjects: | |
| Online Access: | http://www.ijbm.org/articles/i46/ijbm_12(2)_oa17.pdf |
| _version_ | 1817976017000595456 |
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| author | Amr A. Abd-Elghany Ebtesam A. Mohamad Fadel M. Ali Ahmed M. M. Yousef Marwa Samy Nawal Ayman Seham AbdelFatah Eslam Atef Mohamed A. El-Sakhawy |
| author_facet | Amr A. Abd-Elghany Ebtesam A. Mohamad Fadel M. Ali Ahmed M. M. Yousef Marwa Samy Nawal Ayman Seham AbdelFatah Eslam Atef Mohamed A. El-Sakhawy |
| author_sort | Amr A. Abd-Elghany |
| collection | DOAJ |
| description | The aim of this study was to investigate the electric field frequency and the time of exposure that causes maximum inhibition of Escherichia coli (E. coli) growth.
Methods and Results: Bacterial suspensions were subjected to an extremely low frequency electric field (ELFEF) with a 0.1 Hz interval between 0.2 Hz and 0.4 Hz. The bacterial growth was observed through optical density (OD) readings. OD values were taken every hour for four hours to monitor bacterial growth in both exposed and unexposed samples. The antibiotic susceptibility test was done to determine the difference between the susceptibility of both exposed and unexposed bacterial samples. Structural changes in the exposed bacterial samples were monitored by transmission electron microscope (TEM). The bacterial growth curve revealed a highly significant growth inhibition after being exposed to 0.2 Hz at 2 hours’ exposure time. E. coli suspension exposed to ELFEF at inhibition frequency 0.2 Hz showed a significant increase in susceptibility to antibiotics Keflex, meropenem, and piperacillin-tazobactam. The current data suggest that treating E. coli with 0.2 Hz for 2 hours is an effective, prospective, and novel technique for reducing cellular growth and dramatic alteration in the cell membrane. TEM clarified the great destruction of the bacteria cell wall. |
| first_indexed | 2024-04-13T21:57:58Z |
| format | Article |
| id | doaj.art-0636786910b740f889617dbff79c261f |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-04-13T21:57:58Z |
| publishDate | 2022-06-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-0636786910b740f889617dbff79c261f2022-12-22T02:28:12ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292022-06-0112229329810.21103/Article12(2)_OA17Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coliAmr A. Abd-Elghany0Ebtesam A. Mohamad1Fadel M. Ali2Ahmed M. M. Yousef3Marwa Samy4Nawal Ayman5Seham AbdelFatah6Eslam Atef7Mohamed A. El-Sakhawy8Radiology and Medical Imaging Department, College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, Alkharj, Saudi Arabia; Biophysics Department, Faculty of Science, Cairo University,Cairo, EgyptBiophysics Department, Faculty of Science, Cairo University,Cairo, EgyptBiophysics Department, Faculty of Science, Cairo University,Cairo, EgyptRadiology and Medical Imaging Department, College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, Alkharj, Saudi ArabiaBiophysics Department, Faculty of Science, Cairo University,Cairo, EgyptBiophysics Department, Faculty of Science, Cairo University,Cairo, EgyptBiophysics Department, Faculty of Science, Cairo University,Cairo, EgyptBiophysics Department, Faculty of Science, Cairo University,Cairo, EgyptDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, Al-Kharj, Saudi Arabia; Department of Medicinal and Aromatic Plants, Desert Research Center, Cairo, EgyptThe aim of this study was to investigate the electric field frequency and the time of exposure that causes maximum inhibition of Escherichia coli (E. coli) growth. Methods and Results: Bacterial suspensions were subjected to an extremely low frequency electric field (ELFEF) with a 0.1 Hz interval between 0.2 Hz and 0.4 Hz. The bacterial growth was observed through optical density (OD) readings. OD values were taken every hour for four hours to monitor bacterial growth in both exposed and unexposed samples. The antibiotic susceptibility test was done to determine the difference between the susceptibility of both exposed and unexposed bacterial samples. Structural changes in the exposed bacterial samples were monitored by transmission electron microscope (TEM). The bacterial growth curve revealed a highly significant growth inhibition after being exposed to 0.2 Hz at 2 hours’ exposure time. E. coli suspension exposed to ELFEF at inhibition frequency 0.2 Hz showed a significant increase in susceptibility to antibiotics Keflex, meropenem, and piperacillin-tazobactam. The current data suggest that treating E. coli with 0.2 Hz for 2 hours is an effective, prospective, and novel technique for reducing cellular growth and dramatic alteration in the cell membrane. TEM clarified the great destruction of the bacteria cell wall.http://www.ijbm.org/articles/i46/ijbm_12(2)_oa17.pdfextremely low frequency electric fielde. colioptical densitytransmission electron microscope |
| spellingShingle | Amr A. Abd-Elghany Ebtesam A. Mohamad Fadel M. Ali Ahmed M. M. Yousef Marwa Samy Nawal Ayman Seham AbdelFatah Eslam Atef Mohamed A. El-Sakhawy Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli International Journal of Biomedicine extremely low frequency electric field e. coli optical density transmission electron microscope |
| title | Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli |
| title_full | Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli |
| title_fullStr | Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli |
| title_full_unstemmed | Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli |
| title_short | Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli |
| title_sort | antibacterial control of an extremely low frequency electric field on escherichia coli |
| topic | extremely low frequency electric field e. coli optical density transmission electron microscope |
| url | http://www.ijbm.org/articles/i46/ijbm_12(2)_oa17.pdf |
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