Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel
Bioaugmentation is a valuable technique for oil recovery. This study investigates the composition and functions of microbial communities in gasoline- and diesel-contaminated soils of garages Matoko (SGM) and Guy et Paul (SGP) originating from auto mechanic workshops as well as the concentration of s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/722 |
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| author | Emerance Jessica Claire D’Assise Goma-Tchimbakala Ilaria Pietrini Joseph Goma-Tchimbakala Stefano Paolo Corgnati |
| author_facet | Emerance Jessica Claire D’Assise Goma-Tchimbakala Ilaria Pietrini Joseph Goma-Tchimbakala Stefano Paolo Corgnati |
| author_sort | Emerance Jessica Claire D’Assise Goma-Tchimbakala |
| collection | DOAJ |
| description | Bioaugmentation is a valuable technique for oil recovery. This study investigates the composition and functions of microbial communities in gasoline- and diesel-contaminated soils of garages Matoko (SGM) and Guy et Paul (SGP) originating from auto mechanic workshops as well as the concentration of soil enzymes β-glucosidase, β-glucosaminidase, and acid phosphatase. The work aimed to evaluate the presence of petroleum-hydrocarbon-degrading bacteria for the development of foreseen bioremediation of oil-contaminated soils. Microbial diversity, as given by shotgun metagenomics, indicated the presence of 16 classes, among which Actinobacteria and Gammaproteobacteria dominated, as well as more than 50 families, including the dominant Gordoniaceae (26.63%) in SGM and Pseudomonadaceae (57.89%) in SGP. The dominant bacterial genera in the two soils were, respectively, <i>Gordonia</i> (26.7%) and <i>Pseudomonas</i> (57.9%). The exploration of the bacterial metabolic abilities using HUMANn2 allowed to detect genes and pathways involved in alkanes and aromatic hydrocarbons in the two contaminated soils. Furthermore, enzymes β-glucosidase, β-glucosaminidase, and acid phosphatase were found in high concentrations ranging between 90.27 ± 5.3 and 804.17 ± 20.5 µg pN/g soil/h, which indicated active microbial metabolism. The high diversity of microorganisms with a hydrocarbon degradation genetic package revealed that the bacteria inhabiting the two soils are likely good candidates for the bioaugmentation of oil-contaminated soils. |
| first_indexed | 2024-03-09T10:58:48Z |
| format | Article |
| id | doaj.art-063a2fa9582f4014b3532b8c710efa7f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:58:48Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-063a2fa9582f4014b3532b8c710efa7f2023-12-01T01:23:45ZengMDPI AGMicroorganisms2076-26072023-03-0111372210.3390/microorganisms11030722Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel FuelEmerance Jessica Claire D’Assise Goma-Tchimbakala0Ilaria Pietrini1Joseph Goma-Tchimbakala2Stefano Paolo Corgnati3Energy Center Laboratory, Department of Energy (DENERG) Politecnico di Torino, 10138 Torino, ItalyEni R&D, Environmental and Biological Laboratories, Eni SpA, 20097 Milan, ItalyEcole Nationale Supérieure d’Agronomie et de Foresterie, Université Marien Ngouabi, Brazzaville BP 69, CongoEnergy Center Laboratory, Department of Energy (DENERG) Politecnico di Torino, 10138 Torino, ItalyBioaugmentation is a valuable technique for oil recovery. This study investigates the composition and functions of microbial communities in gasoline- and diesel-contaminated soils of garages Matoko (SGM) and Guy et Paul (SGP) originating from auto mechanic workshops as well as the concentration of soil enzymes β-glucosidase, β-glucosaminidase, and acid phosphatase. The work aimed to evaluate the presence of petroleum-hydrocarbon-degrading bacteria for the development of foreseen bioremediation of oil-contaminated soils. Microbial diversity, as given by shotgun metagenomics, indicated the presence of 16 classes, among which Actinobacteria and Gammaproteobacteria dominated, as well as more than 50 families, including the dominant Gordoniaceae (26.63%) in SGM and Pseudomonadaceae (57.89%) in SGP. The dominant bacterial genera in the two soils were, respectively, <i>Gordonia</i> (26.7%) and <i>Pseudomonas</i> (57.9%). The exploration of the bacterial metabolic abilities using HUMANn2 allowed to detect genes and pathways involved in alkanes and aromatic hydrocarbons in the two contaminated soils. Furthermore, enzymes β-glucosidase, β-glucosaminidase, and acid phosphatase were found in high concentrations ranging between 90.27 ± 5.3 and 804.17 ± 20.5 µg pN/g soil/h, which indicated active microbial metabolism. The high diversity of microorganisms with a hydrocarbon degradation genetic package revealed that the bacteria inhabiting the two soils are likely good candidates for the bioaugmentation of oil-contaminated soils.https://www.mdpi.com/2076-2607/11/3/722shotgun metagenomicsdiesel/gasoline contaminationbacterial diversityhydrocarbons pathwaysbioremediation |
| spellingShingle | Emerance Jessica Claire D’Assise Goma-Tchimbakala Ilaria Pietrini Joseph Goma-Tchimbakala Stefano Paolo Corgnati Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel Microorganisms shotgun metagenomics diesel/gasoline contamination bacterial diversity hydrocarbons pathways bioremediation |
| title | Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel |
| title_full | Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel |
| title_fullStr | Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel |
| title_full_unstemmed | Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel |
| title_short | Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel |
| title_sort | use of shotgun metagenomics to assess the microbial diversity and hydrocarbons degrading functions of auto mechanic workshops soils polluted with gasoline and diesel fuel |
| topic | shotgun metagenomics diesel/gasoline contamination bacterial diversity hydrocarbons pathways bioremediation |
| url | https://www.mdpi.com/2076-2607/11/3/722 |
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