A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria.
Cerebral malaria (CM) is a neurological syndrome that includes coma and seizures following malaria parasite infection. The pathophysiology is not fully understood and cannot be accounted for by infection alone: patients still succumb to CM, even if the underlying parasite infection has resolved. To...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-10-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2948515?pdf=render |
| _version_ | 1818036258898706432 |
|---|---|
| author | Ryan W Carroll Mark S Wainwright Kwang-Youn Kim Trilokesh Kidambi Noé D Gómez Terrie Taylor Kasturi Haldar |
| author_facet | Ryan W Carroll Mark S Wainwright Kwang-Youn Kim Trilokesh Kidambi Noé D Gómez Terrie Taylor Kasturi Haldar |
| author_sort | Ryan W Carroll |
| collection | DOAJ |
| description | Cerebral malaria (CM) is a neurological syndrome that includes coma and seizures following malaria parasite infection. The pathophysiology is not fully understood and cannot be accounted for by infection alone: patients still succumb to CM, even if the underlying parasite infection has resolved. To that effect, there is no known adjuvant therapy for CM. Current murine CM (MCM) models do not allow for rapid clinical identification of affected animals following infection. An animal model that more closely mimics the clinical features of human CM would be helpful in elucidating potential mechanisms of disease pathogenesis and evaluating new adjuvant therapies.A quantitative, rapid murine coma and behavior scale (RMCBS) comprised of 10 parameters was developed to assess MCM manifested in C57BL/6 mice infected with Plasmodium berghei ANKA (PbA). Using this method a single mouse can be completely assessed within 3 minutes. The RMCBS enables the operator to follow the evolution of the clinical syndrome, validated here by correlations with intracerebral hemorrhages. It provides a tool by which subjects can be identified as symptomatic prior to the initiation of trial treatment.Since the RMCBS enables an operator to rapidly follow the course of disease, label a subject as affected or not, and correlate the level of illness with neuropathologic injury, it can ultimately be used to guide the initiation of treatment after the onset of cerebral disease (thus emulating the situation in the field). The RMCBS is a tool by which an adjuvant therapy can be objectively assessed. |
| first_indexed | 2024-12-10T07:08:06Z |
| format | Article |
| id | doaj.art-063a549bd2f34708bbaf6d2689187877 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:08:06Z |
| publishDate | 2010-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-063a549bd2f34708bbaf6d26891878772022-12-22T01:58:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-10-0151010.1371/journal.pone.0013124A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria.Ryan W CarrollMark S WainwrightKwang-Youn KimTrilokesh KidambiNoé D GómezTerrie TaylorKasturi HaldarCerebral malaria (CM) is a neurological syndrome that includes coma and seizures following malaria parasite infection. The pathophysiology is not fully understood and cannot be accounted for by infection alone: patients still succumb to CM, even if the underlying parasite infection has resolved. To that effect, there is no known adjuvant therapy for CM. Current murine CM (MCM) models do not allow for rapid clinical identification of affected animals following infection. An animal model that more closely mimics the clinical features of human CM would be helpful in elucidating potential mechanisms of disease pathogenesis and evaluating new adjuvant therapies.A quantitative, rapid murine coma and behavior scale (RMCBS) comprised of 10 parameters was developed to assess MCM manifested in C57BL/6 mice infected with Plasmodium berghei ANKA (PbA). Using this method a single mouse can be completely assessed within 3 minutes. The RMCBS enables the operator to follow the evolution of the clinical syndrome, validated here by correlations with intracerebral hemorrhages. It provides a tool by which subjects can be identified as symptomatic prior to the initiation of trial treatment.Since the RMCBS enables an operator to rapidly follow the course of disease, label a subject as affected or not, and correlate the level of illness with neuropathologic injury, it can ultimately be used to guide the initiation of treatment after the onset of cerebral disease (thus emulating the situation in the field). The RMCBS is a tool by which an adjuvant therapy can be objectively assessed.http://europepmc.org/articles/PMC2948515?pdf=render |
| spellingShingle | Ryan W Carroll Mark S Wainwright Kwang-Youn Kim Trilokesh Kidambi Noé D Gómez Terrie Taylor Kasturi Haldar A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria. PLoS ONE |
| title | A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria. |
| title_full | A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria. |
| title_fullStr | A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria. |
| title_full_unstemmed | A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria. |
| title_short | A rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria. |
| title_sort | rapid murine coma and behavior scale for quantitative assessment of murine cerebral malaria |
| url | http://europepmc.org/articles/PMC2948515?pdf=render |
| work_keys_str_mv | AT ryanwcarroll arapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT markswainwright arapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT kwangyounkim arapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT trilokeshkidambi arapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT noedgomez arapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT terrietaylor arapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT kasturihaldar arapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT ryanwcarroll rapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT markswainwright rapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT kwangyounkim rapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT trilokeshkidambi rapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT noedgomez rapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT terrietaylor rapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria AT kasturihaldar rapidmurinecomaandbehaviorscaleforquantitativeassessmentofmurinecerebralmalaria |