Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria)
The Shiga toxin producing Escherichia coli (STEC) are considered to be one of the most important groups of emerging public health pathogens with cattle being the main reservoir. The objective of this study was to isolate and characterise Escherichia coli Shiga toxins in dairy cattle farms. A total o...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2024-06-01
|
| Series: | Bulgarian Journal of Veterinary Medicine |
| Subjects: |
| _version_ | 1797244675737780224 |
|---|---|
| author | D. Baazize-Ammi S. Kechih-Bounar A. S. Dechicha S. Kebbal I. Gharbi N. Hezil Y. Chebloune D. Guetarni |
| author_facet | D. Baazize-Ammi S. Kechih-Bounar A. S. Dechicha S. Kebbal I. Gharbi N. Hezil Y. Chebloune D. Guetarni |
| author_sort | D. Baazize-Ammi |
| collection | DOAJ |
| description | The Shiga toxin producing Escherichia coli (STEC) are considered to be one of the most important groups of emerging public health pathogens with cattle being the main reservoir. The objective of this study was to isolate and characterise Escherichia coli Shiga toxins in dairy cattle farms. A total of 252 faeces samples were collected from healthy cows belonging to 37 farms. PCR screening of samples for the common sequences of stx1/stx2 genes and stx1 and stx2 genes resulted in a STEC faecal excretion prevalence of 59.5% at the farm level and 26.6% at the individual level. Among positive animals, 85.1% carried STEC with a single stx1 gene and 14.9% with the stx1 and stx2 genes. Immunomagnetic separation was performed on 40 PCR-positive samples (10/10 positive for the stx1 and stx2 genes and 30/57 positive only for stx1). Biochemical identification revealed the presence of 66 E. coli strains (27.5%). The search for virulence genes on these strains by PCR showed that only twenty-two (33.33%) were STEC. The presence of the stx1, stx2, ehx and eae genes was characterised in 30.3%, 4.54%, 13.63% and 1.51% of the strains, respectively, indicating that the virulotype with stx alone was dominant. Serological identification showed the absence of O157 sero-groups and the presence of O1(2), O2, O18(2), O128 sero-groups. The susceptibility testing of STEC showed 68.18% resistance to chloramphenicol, 63.64% to neomycin, 59.1% to ampicillin, 22.73% to trimethoprim + sulfamethoxazole and 9.1% to amoxicillin + clavulanic acid and nalidixic acid. Four strains showed multi-resistance. Bovine carriage of STEC constitutes a public health risk by contamination of milk and meat. To protect human health, it is necessary to limit the bovine STEC shedding. |
| first_indexed | 2024-04-24T19:14:47Z |
| format | Article |
| id | doaj.art-064069d443c8447f9a5fd4518ed0d125 |
| institution | Directory Open Access Journal |
| issn | 1311-1477 1313-3543 |
| language | English |
| last_indexed | 2024-04-24T19:14:47Z |
| publishDate | 2024-06-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj.art-064069d443c8447f9a5fd4518ed0d1252024-03-26T07:32:47ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432024-06-0127219620510.15547/bjvm.2022-0028Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria)D. Baazize-Ammi0S. Kechih-Bounar1A. S. Dechicha2S. Kebbal3I. Gharbi4N. Hezil5Y. Chebloune 6D. Guetarni7Institute of Veterinary Sciences, Blida University 1, AlgeriaRegional Veterinary Laboratory of Draa Ben Khedda, Wilaya of Tizi Ouzou, AlgeriaInstitute of Veterinary Sciences, Blida University 1, AlgeriaInstitute of Veterinary Sciences, Blida University 1, AlgeriaInstitute of Veterinary Sciences, Blida University 1, AlgeriaInstitute of Veterinary Sciences, Blida University 1, AlgeriaPathogenesis and Lentivirus Vaccination Laboratory, PAVAL Lab., University Grenoble Alps, Grenoble Cedex 9, FranceBiology Department, Faculty of Nature and Life Sciences, Blida University 1, AlgeriaThe Shiga toxin producing Escherichia coli (STEC) are considered to be one of the most important groups of emerging public health pathogens with cattle being the main reservoir. The objective of this study was to isolate and characterise Escherichia coli Shiga toxins in dairy cattle farms. A total of 252 faeces samples were collected from healthy cows belonging to 37 farms. PCR screening of samples for the common sequences of stx1/stx2 genes and stx1 and stx2 genes resulted in a STEC faecal excretion prevalence of 59.5% at the farm level and 26.6% at the individual level. Among positive animals, 85.1% carried STEC with a single stx1 gene and 14.9% with the stx1 and stx2 genes. Immunomagnetic separation was performed on 40 PCR-positive samples (10/10 positive for the stx1 and stx2 genes and 30/57 positive only for stx1). Biochemical identification revealed the presence of 66 E. coli strains (27.5%). The search for virulence genes on these strains by PCR showed that only twenty-two (33.33%) were STEC. The presence of the stx1, stx2, ehx and eae genes was characterised in 30.3%, 4.54%, 13.63% and 1.51% of the strains, respectively, indicating that the virulotype with stx alone was dominant. Serological identification showed the absence of O157 sero-groups and the presence of O1(2), O2, O18(2), O128 sero-groups. The susceptibility testing of STEC showed 68.18% resistance to chloramphenicol, 63.64% to neomycin, 59.1% to ampicillin, 22.73% to trimethoprim + sulfamethoxazole and 9.1% to amoxicillin + clavulanic acid and nalidixic acid. Four strains showed multi-resistance. Bovine carriage of STEC constitutes a public health risk by contamination of milk and meat. To protect human health, it is necessary to limit the bovine STEC shedding.algeriacattlefaecesgenesshiga toxin producing escherichia colivirulence |
| spellingShingle | D. Baazize-Ammi S. Kechih-Bounar A. S. Dechicha S. Kebbal I. Gharbi N. Hezil Y. Chebloune D. Guetarni Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria) Bulgarian Journal of Veterinary Medicine algeria cattle faeces genes shiga toxin producing escherichia coli virulence |
| title | Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria) |
| title_full | Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria) |
| title_fullStr | Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria) |
| title_full_unstemmed | Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria) |
| title_short | Isolation and characterisation of Shiga toxins producing Escherichia coli in dairy cows in the governorate of Blida (Algeria) |
| title_sort | isolation and characterisation of shiga toxins producing escherichia coli in dairy cows in the governorate of blida algeria |
| topic | algeria cattle faeces genes shiga toxin producing escherichia coli virulence |
| work_keys_str_mv | AT dbaazizeammi isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria AT skechihbounar isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria AT asdechicha isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria AT skebbal isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria AT igharbi isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria AT nhezil isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria AT ychebloune isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria AT dguetarni isolationandcharacterisationofshigatoxinsproducingescherichiacoliindairycowsinthegovernorateofblidaalgeria |