Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup
Coupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant Arabidopsis thaliana at higher throughput than conventional method...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-04-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00408/full |
| _version_ | 1819266079495553024 |
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| author | Marie-Francoise Noirot-Gros Shalaka V. Shinde Chase Akins Jessica L. Johnson Sarah Zerbs Rosemarie Wilton Kenneth M. Kemner Philippe Noirot Gyorgy Babnigg |
| author_facet | Marie-Francoise Noirot-Gros Shalaka V. Shinde Chase Akins Jessica L. Johnson Sarah Zerbs Rosemarie Wilton Kenneth M. Kemner Philippe Noirot Gyorgy Babnigg |
| author_sort | Marie-Francoise Noirot-Gros |
| collection | DOAJ |
| description | Coupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant Arabidopsis thaliana at higher throughput than conventional methods. However, there is a need for microfluidic devices which enable in vivo studies of root development and RMIs in woody plants. Here, we developed the RMI-chip, a simple microfluidic setup in which Populus tremuloides (aspen tree) seedlings can grow for over a month, allowing continuous microscopic observation of interactions between live roots and rhizobacteria. We find that the colonization of growing aspen roots by Pseudomonas fluorescens in the RMI-chip involves dynamic biofilm formation and dispersal, in keeping with previous observations in a different experimental set-up. Also, we find that whole-cell biosensors based on the rhizobacterium Bacillus subtilis can be used to monitor compositional changes in the rhizosphere but that the application of these biosensors is limited by their efficiency at colonizing aspen roots and persisting. These results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer. |
| first_indexed | 2024-12-23T20:55:34Z |
| format | Article |
| id | doaj.art-06443e54b6914310b2a031e63e8ee730 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T20:55:34Z |
| publishDate | 2020-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-06443e54b6914310b2a031e63e8ee7302022-12-21T17:31:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-04-011110.3389/fpls.2020.00408505518Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics SetupMarie-Francoise Noirot-GrosShalaka V. ShindeChase AkinsJessica L. JohnsonSarah ZerbsRosemarie WiltonKenneth M. KemnerPhilippe NoirotGyorgy BabniggCoupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant Arabidopsis thaliana at higher throughput than conventional methods. However, there is a need for microfluidic devices which enable in vivo studies of root development and RMIs in woody plants. Here, we developed the RMI-chip, a simple microfluidic setup in which Populus tremuloides (aspen tree) seedlings can grow for over a month, allowing continuous microscopic observation of interactions between live roots and rhizobacteria. We find that the colonization of growing aspen roots by Pseudomonas fluorescens in the RMI-chip involves dynamic biofilm formation and dispersal, in keeping with previous observations in a different experimental set-up. Also, we find that whole-cell biosensors based on the rhizobacterium Bacillus subtilis can be used to monitor compositional changes in the rhizosphere but that the application of these biosensors is limited by their efficiency at colonizing aspen roots and persisting. These results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer.https://www.frontiersin.org/article/10.3389/fpls.2020.00408/fullroot-microbe interactionPopulus tremuloidesPseudomonas fluorescensBacillus subtilisbiosensormicrofluidics |
| spellingShingle | Marie-Francoise Noirot-Gros Shalaka V. Shinde Chase Akins Jessica L. Johnson Sarah Zerbs Rosemarie Wilton Kenneth M. Kemner Philippe Noirot Gyorgy Babnigg Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup Frontiers in Plant Science root-microbe interaction Populus tremuloides Pseudomonas fluorescens Bacillus subtilis biosensor microfluidics |
| title | Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup |
| title_full | Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup |
| title_fullStr | Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup |
| title_full_unstemmed | Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup |
| title_short | Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup |
| title_sort | functional imaging of microbial interactions with tree roots using a microfluidics setup |
| topic | root-microbe interaction Populus tremuloides Pseudomonas fluorescens Bacillus subtilis biosensor microfluidics |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00408/full |
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